Antibacterial activity of crude extracts of Thai medicinal plants against clinical isolates of methicillin-resistant Staphylococcus aureus
Aqueous and ethanolic extracts of Acacia catechu, Garcinia mangostana, Impatiens balsamina, Peltophorum pterocarpum, Psidium guajava, Punica granatum, Quercus infectoria, Tamarindus indica, Uncaria gambir, Walsura robusta were primarily tested for their antibacterial activities against 35 clinical i...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2005-08-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://www.sjst.psu.ac.th/journal/Thai-Herbs-pdf/09-Staphylococcus-aureus.pdf |
| _version_ | 1819055926574841856 |
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| author | Kitpipit, L. Voravuthikunchai, S. |
| author_facet | Kitpipit, L. Voravuthikunchai, S. |
| author_sort | Kitpipit, L. |
| collection | DOAJ |
| description | Aqueous and ethanolic extracts of Acacia catechu, Garcinia mangostana, Impatiens balsamina, Peltophorum pterocarpum, Psidium guajava, Punica granatum, Quercus infectoria, Tamarindus indica, Uncaria gambir, Walsura robusta were primarily tested for their antibacterial activities against 35 clinical isolates of methicillin-resistant S. aureus and S. aureus ATCC 25923 using disc diffusion method (2.5 mg/disc). Almost all extracts, except Tamarindus indica exhibited antibacterial activity. Both aqueous and ethanolic extracts of Acacia catechu, Psidium guajava, Punica granatum, Quercus infectoria, and Uncaria gambir, and ethanolic extracts of Garcinia mangostana, Impatiens balsamina, Peltophorum pterocarpum, and Walsura robusta demonstrated inhibition zones, ranging from 6 to 22 mm. Determination of minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values were performed using agar dilution method. The MIC/MBC values of aqueous extracts of Quercus infectoria against clinical isolates of MRSA and S. aureus were 0.2 to 0.4/0.4 to 1.6 and 0.2/1.6 mg/ ml, respectively. Ethanolic extracts of Garcinia mangostana, Punica granatum and Quercus infectoria were demonstrated to be the most effective. The MIC values against MRSA isolates and S. aureus ranged from 0.05 to 0.4 and 0.1, 0.2 to 0.4 and 0.1, 0.2 to 0.4 and 0.2 mg/ml, respectively. The MBC values against MRSA ranged from 0.1 to 0.4, 0.4 to 1.6, and 1.6 to 3.1 mg/ml and against S. aureus at 0.4, 3.2, and 1.6 mg/ml, respectively. |
| first_indexed | 2024-12-21T13:15:17Z |
| format | Article |
| id | doaj.art-278eef37d36c41449fd2720c79f507f3 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-21T13:15:17Z |
| publishDate | 2005-08-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-278eef37d36c41449fd2720c79f507f32022-12-21T19:02:45ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952005-08-0127Suppl.2525534Antibacterial activity of crude extracts of Thai medicinal plants against clinical isolates of methicillin-resistant Staphylococcus aureusKitpipit, L.Voravuthikunchai, S.Aqueous and ethanolic extracts of Acacia catechu, Garcinia mangostana, Impatiens balsamina, Peltophorum pterocarpum, Psidium guajava, Punica granatum, Quercus infectoria, Tamarindus indica, Uncaria gambir, Walsura robusta were primarily tested for their antibacterial activities against 35 clinical isolates of methicillin-resistant S. aureus and S. aureus ATCC 25923 using disc diffusion method (2.5 mg/disc). Almost all extracts, except Tamarindus indica exhibited antibacterial activity. Both aqueous and ethanolic extracts of Acacia catechu, Psidium guajava, Punica granatum, Quercus infectoria, and Uncaria gambir, and ethanolic extracts of Garcinia mangostana, Impatiens balsamina, Peltophorum pterocarpum, and Walsura robusta demonstrated inhibition zones, ranging from 6 to 22 mm. Determination of minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values were performed using agar dilution method. The MIC/MBC values of aqueous extracts of Quercus infectoria against clinical isolates of MRSA and S. aureus were 0.2 to 0.4/0.4 to 1.6 and 0.2/1.6 mg/ ml, respectively. Ethanolic extracts of Garcinia mangostana, Punica granatum and Quercus infectoria were demonstrated to be the most effective. The MIC values against MRSA isolates and S. aureus ranged from 0.05 to 0.4 and 0.1, 0.2 to 0.4 and 0.1, 0.2 to 0.4 and 0.2 mg/ml, respectively. The MBC values against MRSA ranged from 0.1 to 0.4, 0.4 to 1.6, and 1.6 to 3.1 mg/ml and against S. aureus at 0.4, 3.2, and 1.6 mg/ml, respectively.http://www.sjst.psu.ac.th/journal/Thai-Herbs-pdf/09-Staphylococcus-aureus.pdfmethicillin-resistant Staphylococcus aureusMRSAS. aureusmedicinal plantsherbs |
| spellingShingle | Kitpipit, L. Voravuthikunchai, S. Antibacterial activity of crude extracts of Thai medicinal plants against clinical isolates of methicillin-resistant Staphylococcus aureus Songklanakarin Journal of Science and Technology (SJST) methicillin-resistant Staphylococcus aureus MRSA S. aureus medicinal plants herbs |
| title | Antibacterial activity of crude extracts of Thai medicinal plants against clinical isolates of methicillin-resistant Staphylococcus aureus |
| title_full | Antibacterial activity of crude extracts of Thai medicinal plants against clinical isolates of methicillin-resistant Staphylococcus aureus |
| title_fullStr | Antibacterial activity of crude extracts of Thai medicinal plants against clinical isolates of methicillin-resistant Staphylococcus aureus |
| title_full_unstemmed | Antibacterial activity of crude extracts of Thai medicinal plants against clinical isolates of methicillin-resistant Staphylococcus aureus |
| title_short | Antibacterial activity of crude extracts of Thai medicinal plants against clinical isolates of methicillin-resistant Staphylococcus aureus |
| title_sort | antibacterial activity of crude extracts of thai medicinal plants against clinical isolates of methicillin resistant staphylococcus aureus |
| topic | methicillin-resistant Staphylococcus aureus MRSA S. aureus medicinal plants herbs |
| url | http://www.sjst.psu.ac.th/journal/Thai-Herbs-pdf/09-Staphylococcus-aureus.pdf |
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