Changes in Enzyme Efficiency During Lipase-Catalyzed Hydrolysis of Canola Oil in a Supercritical Bioreactor
Enzyme efficiency was investigated in the lipase-catalyzed hydrolysis of canola oil in supercritical carbon dioxide (SCCO2). Immobilized lipase from Mucor miehie (Lipozyme IM) was used as the catalyst and the results showed that enzyme efficiency dropped at high pressures indicating a possible chang...
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| Format: | Article |
| Language: | English |
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Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR
2006-12-01
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| Series: | Iranian Journal of Chemistry & Chemical Engineering |
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| Online Access: | http://www.ijcce.ac.ir/article_7924_0335dc31078c99b31ddaeef10d560d56.pdf |
| _version_ | 1818643279302885376 |
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| author | Karamatollah Rezaei Feral Temelli |
| author_facet | Karamatollah Rezaei Feral Temelli |
| author_sort | Karamatollah Rezaei |
| collection | DOAJ |
| description | Enzyme efficiency was investigated in the lipase-catalyzed hydrolysis of canola oil in supercritical carbon dioxide (SCCO2). Immobilized lipase from Mucor miehie (Lipozyme IM) was used as the catalyst and the results showed that enzyme efficiency dropped at high pressures indicating a possible change in enzyme microstructure. Therefore, scanning electron microscopy (SEM) was used as a supplementary tool to investigate microstructural stability of the enzyme under supercritical conditions. SEM images of the treated enzymes did not demonstrate any apparent structural changes with a change in pressure (24.0 and 38.0 MPa), enzyme load of the reactor (1.0 and 5.0 g), CO2 flow rate (0.5 and 3.9 L/min) and the oil content (extracted from 3.0 and 15.0 g canola flakes) of SCCO2. However, a change at the molecular level is a possibility, which requires further investigation. |
| first_indexed | 2024-12-16T23:56:26Z |
| format | Article |
| id | doaj.art-278f684c0fd4467591a6581d1300bc71 |
| institution | Directory Open Access Journal |
| issn | 1021-9986 1021-9986 |
| language | English |
| last_indexed | 2024-12-16T23:56:26Z |
| publishDate | 2006-12-01 |
| publisher | Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR |
| record_format | Article |
| series | Iranian Journal of Chemistry & Chemical Engineering |
| spelling | doaj.art-278f684c0fd4467591a6581d1300bc712022-12-21T22:11:12ZengIranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECRIranian Journal of Chemistry & Chemical Engineering1021-99861021-99862006-12-0125425357924Changes in Enzyme Efficiency During Lipase-Catalyzed Hydrolysis of Canola Oil in a Supercritical BioreactorKaramatollah Rezaei0Feral Temelli1Department of Food Science & Engineering, Faculty of Agricultural Biosystem Engineering, University of Tehran, P.O. Box 31587-78659 Karaj, I.R. IRANDepartment of Agricultural, Food and Nutritional Science, University of Alberta Edmonton, Alberta, T6G 2P5 CANADAEnzyme efficiency was investigated in the lipase-catalyzed hydrolysis of canola oil in supercritical carbon dioxide (SCCO2). Immobilized lipase from Mucor miehie (Lipozyme IM) was used as the catalyst and the results showed that enzyme efficiency dropped at high pressures indicating a possible change in enzyme microstructure. Therefore, scanning electron microscopy (SEM) was used as a supplementary tool to investigate microstructural stability of the enzyme under supercritical conditions. SEM images of the treated enzymes did not demonstrate any apparent structural changes with a change in pressure (24.0 and 38.0 MPa), enzyme load of the reactor (1.0 and 5.0 g), CO2 flow rate (0.5 and 3.9 L/min) and the oil content (extracted from 3.0 and 15.0 g canola flakes) of SCCO2. However, a change at the molecular level is a possibility, which requires further investigation.http://www.ijcce.ac.ir/article_7924_0335dc31078c99b31ddaeef10d560d56.pdfcanola oilbiocatalysisbioreactorenzyme efficiencyhydrolysismicrostructurescanning electron microscopysupercritical |
| spellingShingle | Karamatollah Rezaei Feral Temelli Changes in Enzyme Efficiency During Lipase-Catalyzed Hydrolysis of Canola Oil in a Supercritical Bioreactor Iranian Journal of Chemistry & Chemical Engineering canola oil biocatalysis bioreactor enzyme efficiency hydrolysis microstructure scanning electron microscopy supercritical |
| title | Changes in Enzyme Efficiency During Lipase-Catalyzed Hydrolysis of Canola Oil in a Supercritical Bioreactor |
| title_full | Changes in Enzyme Efficiency During Lipase-Catalyzed Hydrolysis of Canola Oil in a Supercritical Bioreactor |
| title_fullStr | Changes in Enzyme Efficiency During Lipase-Catalyzed Hydrolysis of Canola Oil in a Supercritical Bioreactor |
| title_full_unstemmed | Changes in Enzyme Efficiency During Lipase-Catalyzed Hydrolysis of Canola Oil in a Supercritical Bioreactor |
| title_short | Changes in Enzyme Efficiency During Lipase-Catalyzed Hydrolysis of Canola Oil in a Supercritical Bioreactor |
| title_sort | changes in enzyme efficiency during lipase catalyzed hydrolysis of canola oil in a supercritical bioreactor |
| topic | canola oil biocatalysis bioreactor enzyme efficiency hydrolysis microstructure scanning electron microscopy supercritical |
| url | http://www.ijcce.ac.ir/article_7924_0335dc31078c99b31ddaeef10d560d56.pdf |
| work_keys_str_mv | AT karamatollahrezaei changesinenzymeefficiencyduringlipasecatalyzedhydrolysisofcanolaoilinasupercriticalbioreactor AT feraltemelli changesinenzymeefficiencyduringlipasecatalyzedhydrolysisofcanolaoilinasupercriticalbioreactor |