Ethylene Signal Is Involved in the Regulation of Anthocyanin Accumulation in Flesh of Postharvest Plums (<i>Prunus salicina</i> Lindl.)
Ethylene is positively correlated with the anthocyanin accumulation in postharvest plum fruit, but the regulation mechanism has not been fully clarified. In this work, the ‘Friar’ plum fruit under different storage temperatures (0, 10 and 25 °C) and treatments (100.0 μL L<sup>−1</sup> et...
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MDPI AG
2023-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/4/893 |
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| author | Xueling Li Yudou Cheng Ying Wang Xiaohe Yang Chuangqi Wei Junfeng Guan |
| author_facet | Xueling Li Yudou Cheng Ying Wang Xiaohe Yang Chuangqi Wei Junfeng Guan |
| author_sort | Xueling Li |
| collection | DOAJ |
| description | Ethylene is positively correlated with the anthocyanin accumulation in postharvest plum fruit, but the regulation mechanism has not been fully clarified. In this work, the ‘Friar’ plum fruit under different storage temperatures (0, 10 and 25 °C) and treatments (100.0 μL L<sup>−1</sup> ethylene and 1.0 μL L<sup>−1</sup> 1-MCP) were applied to study the relationship between anthocyanin accumulation and ethylene signal pathway. The fruits stored at 10 °C had higher ethylene production rate and more anthocyanin in flesh than those stored at 0 °C and 25 °C. Ten ethylene biosynthesis associated genes and forty-one ethylene signal transduction related genes were obtained from the previous transcriptome data. Among them, the expression levels of ethylene biosynthesis associated genes (<i>PsACS1</i>, <i>PsACS4</i> and <i>PsACO1</i>), and ethylene signal transduction related genes (<i>PsERS1s</i>, <i>PsETR2</i>, <i>PsERF1a</i>, and <i>PsERF12</i>) were markedly higher in the fruits stored at 10 °C than those at 0 °C and 25 °C. Exogenous ethylene treatment enhanced while 1-MCP treatment inhibited the anthocyanin accumulation in the flesh under storage at 10 °C. In addition, exogenous ethylene treatment markedly increased the expression levels of <i>PsACS1</i>, <i>PsACS4</i>, <i>PsACO1</i>, <i>PsETR2</i>, <i>PsERF1a</i>, and <i>PsERF12</i> in the flesh once it turning red, as well as the anthocyanin biosynthesis related genes (<i>PsPAL</i>, <i>PsCHS</i>, <i>PsF3H</i>, <i>PsDRF</i>, <i>PsANS</i>, <i>PsUFGT</i> and <i>PsMYB10</i>), whereas 1-MCP treatment manifested the contrary effects. Correlation analysis indicated that there was a significant positive correlation between genes expression related to ethylene signal pathway and anthocyanin biosynthesis, except for <i>PsERF11</i>. In conclusion, ethylene signal pathway is involved in the flesh reddening by up-regulating the anthocyanin biosynthesis related genes. |
| first_indexed | 2024-03-11T08:14:34Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
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| spelling | doaj.art-2791cc2882b945e1adebae0d8de192ad2023-11-16T22:48:55ZengMDPI AGPlants2223-77472023-02-0112489310.3390/plants12040893Ethylene Signal Is Involved in the Regulation of Anthocyanin Accumulation in Flesh of Postharvest Plums (<i>Prunus salicina</i> Lindl.)Xueling Li0Yudou Cheng1Ying Wang2Xiaohe Yang3Chuangqi Wei4Junfeng Guan5Institute of Biotechnology and Food Sciences, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, ChinaInstitute of Biotechnology and Food Sciences, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, ChinaInstitute of Biotechnology and Food Sciences, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, ChinaInstitute of Biotechnology and Food Sciences, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, ChinaInstitute of Biotechnology and Food Sciences, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, ChinaInstitute of Biotechnology and Food Sciences, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, ChinaEthylene is positively correlated with the anthocyanin accumulation in postharvest plum fruit, but the regulation mechanism has not been fully clarified. In this work, the ‘Friar’ plum fruit under different storage temperatures (0, 10 and 25 °C) and treatments (100.0 μL L<sup>−1</sup> ethylene and 1.0 μL L<sup>−1</sup> 1-MCP) were applied to study the relationship between anthocyanin accumulation and ethylene signal pathway. The fruits stored at 10 °C had higher ethylene production rate and more anthocyanin in flesh than those stored at 0 °C and 25 °C. Ten ethylene biosynthesis associated genes and forty-one ethylene signal transduction related genes were obtained from the previous transcriptome data. Among them, the expression levels of ethylene biosynthesis associated genes (<i>PsACS1</i>, <i>PsACS4</i> and <i>PsACO1</i>), and ethylene signal transduction related genes (<i>PsERS1s</i>, <i>PsETR2</i>, <i>PsERF1a</i>, and <i>PsERF12</i>) were markedly higher in the fruits stored at 10 °C than those at 0 °C and 25 °C. Exogenous ethylene treatment enhanced while 1-MCP treatment inhibited the anthocyanin accumulation in the flesh under storage at 10 °C. In addition, exogenous ethylene treatment markedly increased the expression levels of <i>PsACS1</i>, <i>PsACS4</i>, <i>PsACO1</i>, <i>PsETR2</i>, <i>PsERF1a</i>, and <i>PsERF12</i> in the flesh once it turning red, as well as the anthocyanin biosynthesis related genes (<i>PsPAL</i>, <i>PsCHS</i>, <i>PsF3H</i>, <i>PsDRF</i>, <i>PsANS</i>, <i>PsUFGT</i> and <i>PsMYB10</i>), whereas 1-MCP treatment manifested the contrary effects. Correlation analysis indicated that there was a significant positive correlation between genes expression related to ethylene signal pathway and anthocyanin biosynthesis, except for <i>PsERF11</i>. In conclusion, ethylene signal pathway is involved in the flesh reddening by up-regulating the anthocyanin biosynthesis related genes.https://www.mdpi.com/2223-7747/12/4/893plumethyleneanthocyanin accumulationgene expression |
| spellingShingle | Xueling Li Yudou Cheng Ying Wang Xiaohe Yang Chuangqi Wei Junfeng Guan Ethylene Signal Is Involved in the Regulation of Anthocyanin Accumulation in Flesh of Postharvest Plums (<i>Prunus salicina</i> Lindl.) Plants plum ethylene anthocyanin accumulation gene expression |
| title | Ethylene Signal Is Involved in the Regulation of Anthocyanin Accumulation in Flesh of Postharvest Plums (<i>Prunus salicina</i> Lindl.) |
| title_full | Ethylene Signal Is Involved in the Regulation of Anthocyanin Accumulation in Flesh of Postharvest Plums (<i>Prunus salicina</i> Lindl.) |
| title_fullStr | Ethylene Signal Is Involved in the Regulation of Anthocyanin Accumulation in Flesh of Postharvest Plums (<i>Prunus salicina</i> Lindl.) |
| title_full_unstemmed | Ethylene Signal Is Involved in the Regulation of Anthocyanin Accumulation in Flesh of Postharvest Plums (<i>Prunus salicina</i> Lindl.) |
| title_short | Ethylene Signal Is Involved in the Regulation of Anthocyanin Accumulation in Flesh of Postharvest Plums (<i>Prunus salicina</i> Lindl.) |
| title_sort | ethylene signal is involved in the regulation of anthocyanin accumulation in flesh of postharvest plums i prunus salicina i lindl |
| topic | plum ethylene anthocyanin accumulation gene expression |
| url | https://www.mdpi.com/2223-7747/12/4/893 |
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