Enhanced Abiotic Stress Tolerance of <i>Vicia faba</i> L. Plants Heterologously Expressing the <i>PR10a</i> Gene from Potato
Pathogenesis-related (PR) proteins are known to play relevant roles in plant defense against biotic and abiotic stresses. In the present study, we characterize the response of transgenic faba bean (<i>Vicia faba</i> L.) plants encoding a <i>PR10a</i> gene from potato (<i&g...
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MDPI AG
2021-01-01
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| author | Abeer F. Desouky Ahmed H. Hanafy Ahmed Hartmut Stützel Hans-Jörg Jacobsen Yi-Chen Pao Moemen S. Hanafy |
| author_facet | Abeer F. Desouky Ahmed H. Hanafy Ahmed Hartmut Stützel Hans-Jörg Jacobsen Yi-Chen Pao Moemen S. Hanafy |
| author_sort | Abeer F. Desouky |
| collection | DOAJ |
| description | Pathogenesis-related (PR) proteins are known to play relevant roles in plant defense against biotic and abiotic stresses. In the present study, we characterize the response of transgenic faba bean (<i>Vicia faba</i> L.) plants encoding a <i>PR10a</i> gene from potato (<i>Solanum tuberosum</i> L.) to salinity and drought. The transgene was under the mannopine synthetase (pMAS) promoter. <i>PR10a</i>-overexpressing faba bean plants showed better growth than the wild-type plants after 14 days of drought stress and 30 days of salt stress under hydroponic growth conditions. After removing the stress, the PR10a-plants returned to a normal state, while the wild-type plants could not be restored. Most importantly, there was no phenotypic difference between transgenic and non-transgenic faba bean plants under well-watered conditions. Evaluation of physiological parameters during salt stress showed lower Na<sup>+</sup>-content in the leaves of the transgenic plants, which would reduce the toxic effect. In addition, PR10a-plants were able to maintain vegetative growth and experienced fewer photosystem changes under both stresses and a lower level of osmotic stress injury under salt stress compared to wild-type plants. Taken together, our findings suggest that the <i>PR10a</i> gene from potato plays an important role in abiotic stress tolerance, probably by activation of stress-related physiological processes. |
| first_indexed | 2024-03-09T04:23:40Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T04:23:40Z |
| publishDate | 2021-01-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-27950626624d4abcb5cdbba267cd58e02023-12-03T13:42:46ZengMDPI AGPlants2223-77472021-01-0110117310.3390/plants10010173Enhanced Abiotic Stress Tolerance of <i>Vicia faba</i> L. Plants Heterologously Expressing the <i>PR10a</i> Gene from PotatoAbeer F. Desouky0Ahmed H. Hanafy Ahmed1Hartmut Stützel2Hans-Jörg Jacobsen3Yi-Chen Pao4Moemen S. Hanafy5Plant Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Centre (NRC), Tahrir Str. Dokki, 12311 Cairo, EgyptAgricultural Botany Department, Plant Physiology Section, Faculty of Agriculture, Cairo University, 12613 Giza, EgyptInstitute of Horticultural Production Systems, Leibniz Universität Hannover, Herrenhäuser Straße 2, 30419 Hannover, GermanyInstitute of Plant Genetics, Section of Plant Biotechnology, Leibniz Universität Hannover, Herrenhäuser Str. 2, 30419 Hannover, GermanyInstitute of Horticultural Production Systems, Leibniz Universität Hannover, Herrenhäuser Straße 2, 30419 Hannover, GermanyPlant Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Centre (NRC), Tahrir Str. Dokki, 12311 Cairo, EgyptPathogenesis-related (PR) proteins are known to play relevant roles in plant defense against biotic and abiotic stresses. In the present study, we characterize the response of transgenic faba bean (<i>Vicia faba</i> L.) plants encoding a <i>PR10a</i> gene from potato (<i>Solanum tuberosum</i> L.) to salinity and drought. The transgene was under the mannopine synthetase (pMAS) promoter. <i>PR10a</i>-overexpressing faba bean plants showed better growth than the wild-type plants after 14 days of drought stress and 30 days of salt stress under hydroponic growth conditions. After removing the stress, the PR10a-plants returned to a normal state, while the wild-type plants could not be restored. Most importantly, there was no phenotypic difference between transgenic and non-transgenic faba bean plants under well-watered conditions. Evaluation of physiological parameters during salt stress showed lower Na<sup>+</sup>-content in the leaves of the transgenic plants, which would reduce the toxic effect. In addition, PR10a-plants were able to maintain vegetative growth and experienced fewer photosystem changes under both stresses and a lower level of osmotic stress injury under salt stress compared to wild-type plants. Taken together, our findings suggest that the <i>PR10a</i> gene from potato plays an important role in abiotic stress tolerance, probably by activation of stress-related physiological processes.https://www.mdpi.com/2223-7747/10/1/173abiotic stressgas exchangepathogenesis-related (PR) proteinstransgenic plant<i>Vicia faba</i> L. |
| spellingShingle | Abeer F. Desouky Ahmed H. Hanafy Ahmed Hartmut Stützel Hans-Jörg Jacobsen Yi-Chen Pao Moemen S. Hanafy Enhanced Abiotic Stress Tolerance of <i>Vicia faba</i> L. Plants Heterologously Expressing the <i>PR10a</i> Gene from Potato Plants abiotic stress gas exchange pathogenesis-related (PR) proteins transgenic plant <i>Vicia faba</i> L. |
| title | Enhanced Abiotic Stress Tolerance of <i>Vicia faba</i> L. Plants Heterologously Expressing the <i>PR10a</i> Gene from Potato |
| title_full | Enhanced Abiotic Stress Tolerance of <i>Vicia faba</i> L. Plants Heterologously Expressing the <i>PR10a</i> Gene from Potato |
| title_fullStr | Enhanced Abiotic Stress Tolerance of <i>Vicia faba</i> L. Plants Heterologously Expressing the <i>PR10a</i> Gene from Potato |
| title_full_unstemmed | Enhanced Abiotic Stress Tolerance of <i>Vicia faba</i> L. Plants Heterologously Expressing the <i>PR10a</i> Gene from Potato |
| title_short | Enhanced Abiotic Stress Tolerance of <i>Vicia faba</i> L. Plants Heterologously Expressing the <i>PR10a</i> Gene from Potato |
| title_sort | enhanced abiotic stress tolerance of i vicia faba i l plants heterologously expressing the i pr10a i gene from potato |
| topic | abiotic stress gas exchange pathogenesis-related (PR) proteins transgenic plant <i>Vicia faba</i> L. |
| url | https://www.mdpi.com/2223-7747/10/1/173 |
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