Toxic effects of VCD on kidneys and liver tissues: a histopathological and biochemical study
Abstract Objective We explored detrimental effects of VCD on non-ovarian tissues such as kidneys and liver 14 days post-drug administration. Twelve rats were randomly assigned into two groups. In VCD group, rats received 160 mg/kgbw VCD intraperitoneally for 15 consequent days. Control rats were inj...
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| Format: | Article |
| Language: | English |
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BMC
2019-07-01
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| Series: | BMC Research Notes |
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| Online Access: | http://link.springer.com/article/10.1186/s13104-019-4490-y |
| _version_ | 1818279444785135616 |
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| author | Shahin Ahmadian Sepideh Sheshpari Mahdi Mahdipour Mohammad Pazhang Pei-Shiue Jason Tsai Mohammad Nouri Reza Rahbarghazi Mahnaz Shahnazi |
| author_facet | Shahin Ahmadian Sepideh Sheshpari Mahdi Mahdipour Mohammad Pazhang Pei-Shiue Jason Tsai Mohammad Nouri Reza Rahbarghazi Mahnaz Shahnazi |
| author_sort | Shahin Ahmadian |
| collection | DOAJ |
| description | Abstract Objective We explored detrimental effects of VCD on non-ovarian tissues such as kidneys and liver 14 days post-drug administration. Twelve rats were randomly assigned into two groups. In VCD group, rats received 160 mg/kgbw VCD intraperitoneally for 15 consequent days. Control rats were injected with VCD-free normal saline. At the respective time point, rats were euthanized, blood and tissue samples were collected. H&E staining was performed to evaluate pathological changes. Serum level of ALT, AST, creatinine and urea were also measured. Results Histological analysis revealed hyperemia and follicular atresia in the ovaries, indicating successful POF induction in rats. In renal tissue, extensive tubular necrosis, focal hemorrhage, hyaline casts, and interstitial nephritis were observed. Analysis of hepatic tissue showed numerous hemorrhagic foci, chronic cholangitis, and hepatocyte necrosis, indicating apparent VCD toxicity of both hepatic and renal tissues. The biochemical evaluation revealed a tendency of increase in ALT, AST, creatinine, and Urea in VCD-treated rats; however, the values did not reach significant level. In conclusion, the induction of POF in rats by VCD correlates with renal and hepatic damages. Commensurate with data from this study, any conclusions from experiments based on VCD-induced premature ovarian failure rats should be reported with caution. |
| first_indexed | 2024-12-12T23:33:26Z |
| format | Article |
| id | doaj.art-279a9adf586a4e579c1da8e75f32c06e |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-12T23:33:26Z |
| publishDate | 2019-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-279a9adf586a4e579c1da8e75f32c06e2022-12-22T00:07:37ZengBMCBMC Research Notes1756-05002019-07-011211610.1186/s13104-019-4490-yToxic effects of VCD on kidneys and liver tissues: a histopathological and biochemical studyShahin Ahmadian0Sepideh Sheshpari1Mahdi Mahdipour2Mohammad Pazhang3Pei-Shiue Jason Tsai4Mohammad Nouri5Reza Rahbarghazi6Mahnaz Shahnazi7Women’s Reproductive Health Research Center, Tabriz University of Medical SciencesDepartment of Midwifery, Faculty of Nursing and Midwifery, Tabriz University of Medical SciencesStem Cell Research Center, Tabriz University of Medical SciencesDepartment of Biology, Faculty of Science, Azarbaijan Shahid Madani UniversityCenter for Developmental Biology and Regenerative Medicine Research, National Taiwan University/NTUStem Cell Research Center, Tabriz University of Medical SciencesStem Cell Research Center, Tabriz University of Medical SciencesDepartment of Midwifery, Faculty of Nursing and Midwifery, Tabriz University of Medical SciencesAbstract Objective We explored detrimental effects of VCD on non-ovarian tissues such as kidneys and liver 14 days post-drug administration. Twelve rats were randomly assigned into two groups. In VCD group, rats received 160 mg/kgbw VCD intraperitoneally for 15 consequent days. Control rats were injected with VCD-free normal saline. At the respective time point, rats were euthanized, blood and tissue samples were collected. H&E staining was performed to evaluate pathological changes. Serum level of ALT, AST, creatinine and urea were also measured. Results Histological analysis revealed hyperemia and follicular atresia in the ovaries, indicating successful POF induction in rats. In renal tissue, extensive tubular necrosis, focal hemorrhage, hyaline casts, and interstitial nephritis were observed. Analysis of hepatic tissue showed numerous hemorrhagic foci, chronic cholangitis, and hepatocyte necrosis, indicating apparent VCD toxicity of both hepatic and renal tissues. The biochemical evaluation revealed a tendency of increase in ALT, AST, creatinine, and Urea in VCD-treated rats; however, the values did not reach significant level. In conclusion, the induction of POF in rats by VCD correlates with renal and hepatic damages. Commensurate with data from this study, any conclusions from experiments based on VCD-induced premature ovarian failure rats should be reported with caution.http://link.springer.com/article/10.1186/s13104-019-4490-yKidneysLiverPremature ovarian failureSide effectsToxicityVinyl cyclohexene dioxide |
| spellingShingle | Shahin Ahmadian Sepideh Sheshpari Mahdi Mahdipour Mohammad Pazhang Pei-Shiue Jason Tsai Mohammad Nouri Reza Rahbarghazi Mahnaz Shahnazi Toxic effects of VCD on kidneys and liver tissues: a histopathological and biochemical study BMC Research Notes Kidneys Liver Premature ovarian failure Side effects Toxicity Vinyl cyclohexene dioxide |
| title | Toxic effects of VCD on kidneys and liver tissues: a histopathological and biochemical study |
| title_full | Toxic effects of VCD on kidneys and liver tissues: a histopathological and biochemical study |
| title_fullStr | Toxic effects of VCD on kidneys and liver tissues: a histopathological and biochemical study |
| title_full_unstemmed | Toxic effects of VCD on kidneys and liver tissues: a histopathological and biochemical study |
| title_short | Toxic effects of VCD on kidneys and liver tissues: a histopathological and biochemical study |
| title_sort | toxic effects of vcd on kidneys and liver tissues a histopathological and biochemical study |
| topic | Kidneys Liver Premature ovarian failure Side effects Toxicity Vinyl cyclohexene dioxide |
| url | http://link.springer.com/article/10.1186/s13104-019-4490-y |
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