A simple protocol for venom peptide barcoding in scorpions
Scorpion venoms contain many species-specific peptides which target ion channels in cell membranes. Without harming the scorpions, these peptides can easily be extracted and detected by MALDI-TOF mass spectrometry. So far, only few studies compared the venom of different species solely for taxonomic...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-06-01
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| Series: | EuPA Open Proteomics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212968514000221 |
| _version_ | 1818985114830372864 |
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| author | Stephan Schaffrath Reinhard Predel |
| author_facet | Stephan Schaffrath Reinhard Predel |
| author_sort | Stephan Schaffrath |
| collection | DOAJ |
| description | Scorpion venoms contain many species-specific peptides which target ion channels in cell membranes. Without harming the scorpions, these peptides can easily be extracted and detected by MALDI-TOF mass spectrometry. So far, only few studies compared the venom of different species solely for taxonomic purposes. Here, we describe a very simple protocol for venom extraction and mass fingerprinting that was developed for peptide barcoding (venom code for species identification) and facilitates reproducibility if sample preparation is performed under field conditions. This approach may serve as suitable basis for a taxonomy-oriented scorpion toxin database that interacts with MALDI-TOF mass spectra. |
| first_indexed | 2024-12-20T18:29:45Z |
| format | Article |
| id | doaj.art-279dc8ed668648c797961c503c379cfd |
| institution | Directory Open Access Journal |
| issn | 2212-9685 |
| language | English |
| last_indexed | 2024-12-20T18:29:45Z |
| publishDate | 2014-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | EuPA Open Proteomics |
| spelling | doaj.art-279dc8ed668648c797961c503c379cfd2022-12-21T19:30:04ZengElsevierEuPA Open Proteomics2212-96852014-06-013C23924510.1016/j.euprot.2014.02.017A simple protocol for venom peptide barcoding in scorpionsStephan SchaffrathReinhard PredelScorpion venoms contain many species-specific peptides which target ion channels in cell membranes. Without harming the scorpions, these peptides can easily be extracted and detected by MALDI-TOF mass spectrometry. So far, only few studies compared the venom of different species solely for taxonomic purposes. Here, we describe a very simple protocol for venom extraction and mass fingerprinting that was developed for peptide barcoding (venom code for species identification) and facilitates reproducibility if sample preparation is performed under field conditions. This approach may serve as suitable basis for a taxonomy-oriented scorpion toxin database that interacts with MALDI-TOF mass spectra.http://www.sciencedirect.com/science/article/pii/S2212968514000221HeterometrusScorpionVenom peptidesPeptidomicsMALDI-TOF mass spectrometryMass fingerprint |
| spellingShingle | Stephan Schaffrath Reinhard Predel A simple protocol for venom peptide barcoding in scorpions EuPA Open Proteomics Heterometrus Scorpion Venom peptides Peptidomics MALDI-TOF mass spectrometry Mass fingerprint |
| title | A simple protocol for venom peptide barcoding in scorpions |
| title_full | A simple protocol for venom peptide barcoding in scorpions |
| title_fullStr | A simple protocol for venom peptide barcoding in scorpions |
| title_full_unstemmed | A simple protocol for venom peptide barcoding in scorpions |
| title_short | A simple protocol for venom peptide barcoding in scorpions |
| title_sort | simple protocol for venom peptide barcoding in scorpions |
| topic | Heterometrus Scorpion Venom peptides Peptidomics MALDI-TOF mass spectrometry Mass fingerprint |
| url | http://www.sciencedirect.com/science/article/pii/S2212968514000221 |
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