3D-bioprinted alginate-based bioink scaffolds with β-tricalcium phosphate for bone regeneration applications
Background/purpose: 3D-printed bone tissue engineering is becoming recognized as a key approach in dentistry for creating customized bone regeneration treatments fitting patients bone defects requirements. 3D bioprinting offers an innovative method to fabricate detailed 3D structures, closely emulat...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Journal of Dental Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790223004154 |
| _version_ | 1827292702352343040 |
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| author | Yi-Fan Wu Ya-Ting Wen Eisner Salamanca Lwin Moe Aung Yan-Qiao Chao Chih-Yun Chen Ying-Sui Sun Wei-Jen Chang |
| author_facet | Yi-Fan Wu Ya-Ting Wen Eisner Salamanca Lwin Moe Aung Yan-Qiao Chao Chih-Yun Chen Ying-Sui Sun Wei-Jen Chang |
| author_sort | Yi-Fan Wu |
| collection | DOAJ |
| description | Background/purpose: 3D-printed bone tissue engineering is becoming recognized as a key approach in dentistry for creating customized bone regeneration treatments fitting patients bone defects requirements. 3D bioprinting offers an innovative method to fabricate detailed 3D structures, closely emulating the native bone micro-environment and better bone regeneration. This study aimed to develop an 3D-bioprintable scaffold using a combination of alginate and β-tricalcium phosphate (β-TCP) with the Cellink® BioX printer, aiming to advance the field of tissue engineering. Materials and methods: The physical and biological properties of the resulting 3D-printed scaffolds were evaluated at 10 %, 12 %, and 15 % alginate combined with 10 % β-TCP. The scaffolds were characterized through printability, swelling behavior, degradability, and element analysis. The biological assessment included cell viability, alkaline phosphatase (ALP) activity. Results: 10 % alginate/β-TCP 3D printed at 25 °C scaffold demonstrated the optimal condition for printability, swelling capability, and degradability of cell growth and nutrient diffusion. Addition of β-TCP particles significantly improved the 3D printed material viscosity over only alginate (P < 0.05). 10 % alginate/β-TCP enhanced MG-63 cell's proliferation (P < 0.05) and alkaline phosphatase activity (P < 0.001). Conclusion: This study demonstrated in vitro that 10 % alginate/β-TCP bioink characteristic for fabricating 3D acellular bioprinted scaffolds was the best approach. 10 % alginate/β-TCP bioink 3D-printed scaffold exhibited superior physical properties and promoted enhanced cell viability and alkaline phosphatase activity, showing great potential for personalized bone regeneration treatments. |
| first_indexed | 2024-04-24T13:11:25Z |
| format | Article |
| id | doaj.art-279e387bdabd49e0919a4077230f3902 |
| institution | Directory Open Access Journal |
| issn | 1991-7902 |
| language | English |
| last_indexed | 2024-04-24T13:11:25Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dental Sciences |
| spelling | doaj.art-279e387bdabd49e0919a4077230f39022024-04-05T04:40:23ZengElsevierJournal of Dental Sciences1991-79022024-04-01192111611253D-bioprinted alginate-based bioink scaffolds with β-tricalcium phosphate for bone regeneration applicationsYi-Fan Wu0Ya-Ting Wen1Eisner Salamanca2Lwin Moe Aung3Yan-Qiao Chao4Chih-Yun Chen5Ying-Sui Sun6Wei-Jen Chang7School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan; Department of Biomedical Engineering, Ming-Chuan University, Taoyuan, TaiwanDepartment of Medical Education, Taichung Veterans General Hospital, Taichung, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, TaiwanSchool of Oral Hygiene, College of Oral Medicine, Taipei Medical University, Taipei, TaiwanSchool of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan; Corresponding author. School of Dental Technology, College of Oral Medicine, Taipei Medical University, No. 250 Wu-Hsing Street, Taipei, 11031, Taiwan.School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan; Dental Department, Shuang-Ho Hospital, Taipei Medical University, New Taipei, Taiwan; Corresponding author. School of Dentistry, College of Oral Medicine, Taipei Medical University, No. 250 Wu-Hsing Street, Taipei, 11031, Taiwan.Background/purpose: 3D-printed bone tissue engineering is becoming recognized as a key approach in dentistry for creating customized bone regeneration treatments fitting patients bone defects requirements. 3D bioprinting offers an innovative method to fabricate detailed 3D structures, closely emulating the native bone micro-environment and better bone regeneration. This study aimed to develop an 3D-bioprintable scaffold using a combination of alginate and β-tricalcium phosphate (β-TCP) with the Cellink® BioX printer, aiming to advance the field of tissue engineering. Materials and methods: The physical and biological properties of the resulting 3D-printed scaffolds were evaluated at 10 %, 12 %, and 15 % alginate combined with 10 % β-TCP. The scaffolds were characterized through printability, swelling behavior, degradability, and element analysis. The biological assessment included cell viability, alkaline phosphatase (ALP) activity. Results: 10 % alginate/β-TCP 3D printed at 25 °C scaffold demonstrated the optimal condition for printability, swelling capability, and degradability of cell growth and nutrient diffusion. Addition of β-TCP particles significantly improved the 3D printed material viscosity over only alginate (P < 0.05). 10 % alginate/β-TCP enhanced MG-63 cell's proliferation (P < 0.05) and alkaline phosphatase activity (P < 0.001). Conclusion: This study demonstrated in vitro that 10 % alginate/β-TCP bioink characteristic for fabricating 3D acellular bioprinted scaffolds was the best approach. 10 % alginate/β-TCP bioink 3D-printed scaffold exhibited superior physical properties and promoted enhanced cell viability and alkaline phosphatase activity, showing great potential for personalized bone regeneration treatments.http://www.sciencedirect.com/science/article/pii/S1991790223004154AlginateBeta-tricalcium phosphate (β-TCP)BioprintingBone regeneration |
| spellingShingle | Yi-Fan Wu Ya-Ting Wen Eisner Salamanca Lwin Moe Aung Yan-Qiao Chao Chih-Yun Chen Ying-Sui Sun Wei-Jen Chang 3D-bioprinted alginate-based bioink scaffolds with β-tricalcium phosphate for bone regeneration applications Journal of Dental Sciences Alginate Beta-tricalcium phosphate (β-TCP) Bioprinting Bone regeneration |
| title | 3D-bioprinted alginate-based bioink scaffolds with β-tricalcium phosphate for bone regeneration applications |
| title_full | 3D-bioprinted alginate-based bioink scaffolds with β-tricalcium phosphate for bone regeneration applications |
| title_fullStr | 3D-bioprinted alginate-based bioink scaffolds with β-tricalcium phosphate for bone regeneration applications |
| title_full_unstemmed | 3D-bioprinted alginate-based bioink scaffolds with β-tricalcium phosphate for bone regeneration applications |
| title_short | 3D-bioprinted alginate-based bioink scaffolds with β-tricalcium phosphate for bone regeneration applications |
| title_sort | 3d bioprinted alginate based bioink scaffolds with β tricalcium phosphate for bone regeneration applications |
| topic | Alginate Beta-tricalcium phosphate (β-TCP) Bioprinting Bone regeneration |
| url | http://www.sciencedirect.com/science/article/pii/S1991790223004154 |
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