Cloning and Expression Analysis of <i>HAT1</i> and <i>HDAC1</i> in the Testes of Mature Yaks and Their Sterile Hybrids
The objective of this study was to explore the molecular mechanism of male sterility in yak hybrids based on <i>HAT1</i> and <i>HDAC1</i>. Total RNA was extracted from the testes of adult yaks (<i>n</i> = 11) and sterile cattle-yaks (<i>n</i> = 11) fol...
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MDPI AG
2022-08-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/16/2018 |
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| author | Shijie Sun Zhenhua Shen Suyu Jin Lin Huang Yucai Zheng |
| author_facet | Shijie Sun Zhenhua Shen Suyu Jin Lin Huang Yucai Zheng |
| author_sort | Shijie Sun |
| collection | DOAJ |
| description | The objective of this study was to explore the molecular mechanism of male sterility in yak hybrids based on <i>HAT1</i> and <i>HDAC1</i>. Total RNA was extracted from the testes of adult yaks (<i>n</i> = 11) and sterile cattle-yaks (<i>n</i> = 11) followed by reverse transcription. The coding sequence (CDS) of yak <i>HAT1</i> and <i>HDAC1</i> were obtained by conventional polymerase chain reaction (PCR) and gene cloning. The testicular mRNA and protein levels of <i>HAT1</i> and <i>HDAC1</i> in yaks and cattle-yaks were detected by quantitative PCR (qPCR) and Western blotting, respectively, and the histone H3 lysine 9 (H3K9) histone acetylation level in the testes of yaks and cattle-yaks was assayed using enzyme linked immunosorbent assay (ELISA). The results showed that the CDS of <i>HAT1</i> and <i>HDAC1</i> were 1242 bp and 1449 bp in length, encoding 413 and 482 amino acids, respectively; yaks had a similar mRNA sequence as cattle in both genes. The testicular mRNA and protein levels of <i>HAT1</i> of cattle-yaks were significantly lower than those of yaks, and the protein level of <i>HDAC1</i> was significantly higher than that of yaks. ELISA showed that the acetylation level of testicular H3K9 was significantly lower in yak hybrids than that of yaks. The present results suggest that the decreased level of <i>HAT1</i> and increased level of <i>HDAC1</i> may result in the decreased H3K9 acetylation in cattle-yaks and might be associated with their sterility. |
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| language | English |
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| publishDate | 2022-08-01 |
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| series | Animals |
| spelling | doaj.art-279e7784c36f4c6bbf985f75c4156fd12023-12-01T23:18:49ZengMDPI AGAnimals2076-26152022-08-011216201810.3390/ani12162018Cloning and Expression Analysis of <i>HAT1</i> and <i>HDAC1</i> in the Testes of Mature Yaks and Their Sterile HybridsShijie Sun0Zhenhua Shen1Suyu Jin2Lin Huang3Yucai Zheng4College of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaCollege of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, ChinaThe objective of this study was to explore the molecular mechanism of male sterility in yak hybrids based on <i>HAT1</i> and <i>HDAC1</i>. Total RNA was extracted from the testes of adult yaks (<i>n</i> = 11) and sterile cattle-yaks (<i>n</i> = 11) followed by reverse transcription. The coding sequence (CDS) of yak <i>HAT1</i> and <i>HDAC1</i> were obtained by conventional polymerase chain reaction (PCR) and gene cloning. The testicular mRNA and protein levels of <i>HAT1</i> and <i>HDAC1</i> in yaks and cattle-yaks were detected by quantitative PCR (qPCR) and Western blotting, respectively, and the histone H3 lysine 9 (H3K9) histone acetylation level in the testes of yaks and cattle-yaks was assayed using enzyme linked immunosorbent assay (ELISA). The results showed that the CDS of <i>HAT1</i> and <i>HDAC1</i> were 1242 bp and 1449 bp in length, encoding 413 and 482 amino acids, respectively; yaks had a similar mRNA sequence as cattle in both genes. The testicular mRNA and protein levels of <i>HAT1</i> of cattle-yaks were significantly lower than those of yaks, and the protein level of <i>HDAC1</i> was significantly higher than that of yaks. ELISA showed that the acetylation level of testicular H3K9 was significantly lower in yak hybrids than that of yaks. The present results suggest that the decreased level of <i>HAT1</i> and increased level of <i>HDAC1</i> may result in the decreased H3K9 acetylation in cattle-yaks and might be associated with their sterility.https://www.mdpi.com/2076-2615/12/16/2018epigeneticscattle-yakmale sterility<i>HAT1</i><i>HDAC1</i>H3K9 acetylation |
| spellingShingle | Shijie Sun Zhenhua Shen Suyu Jin Lin Huang Yucai Zheng Cloning and Expression Analysis of <i>HAT1</i> and <i>HDAC1</i> in the Testes of Mature Yaks and Their Sterile Hybrids Animals epigenetics cattle-yak male sterility <i>HAT1</i> <i>HDAC1</i> H3K9 acetylation |
| title | Cloning and Expression Analysis of <i>HAT1</i> and <i>HDAC1</i> in the Testes of Mature Yaks and Their Sterile Hybrids |
| title_full | Cloning and Expression Analysis of <i>HAT1</i> and <i>HDAC1</i> in the Testes of Mature Yaks and Their Sterile Hybrids |
| title_fullStr | Cloning and Expression Analysis of <i>HAT1</i> and <i>HDAC1</i> in the Testes of Mature Yaks and Their Sterile Hybrids |
| title_full_unstemmed | Cloning and Expression Analysis of <i>HAT1</i> and <i>HDAC1</i> in the Testes of Mature Yaks and Their Sterile Hybrids |
| title_short | Cloning and Expression Analysis of <i>HAT1</i> and <i>HDAC1</i> in the Testes of Mature Yaks and Their Sterile Hybrids |
| title_sort | cloning and expression analysis of i hat1 i and i hdac1 i in the testes of mature yaks and their sterile hybrids |
| topic | epigenetics cattle-yak male sterility <i>HAT1</i> <i>HDAC1</i> H3K9 acetylation |
| url | https://www.mdpi.com/2076-2615/12/16/2018 |
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