Topical Digitoxigenin for Wound Healing: A Feasibility Study
(1) Background: Cardiotonic steroids have been found to stimulate collagen synthesis and might be potential wound healing therapeutics. The objective of this study was to evaluate the feasibility of digitoxigenin and its topical formulation for wound healing; (2) Methods: In the in vitro study, the...
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| Format: | Article |
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MDPI AG
2018-03-01
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| Series: | Bioengineering |
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| Online Access: | http://www.mdpi.com/2306-5354/5/1/21 |
| _version_ | 1797724385414479872 |
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| author | Xinchi Feng Cuifen Wang Yunhui Xu Joel Turley Zijian Xie Sandrine V. Pierre Jinsong Hao |
| author_facet | Xinchi Feng Cuifen Wang Yunhui Xu Joel Turley Zijian Xie Sandrine V. Pierre Jinsong Hao |
| author_sort | Xinchi Feng |
| collection | DOAJ |
| description | (1) Background: Cardiotonic steroids have been found to stimulate collagen synthesis and might be potential wound healing therapeutics. The objective of this study was to evaluate the feasibility of digitoxigenin and its topical formulation for wound healing; (2) Methods: In the in vitro study, the human dermal fibroblast cells were treated with digitoxigenin and collagen synthesis was assessed. In the in vivo study, digitoxigenin was applied to excisional full-thickness wounds in rats immediately after wounding and remained for three days, and wound open was evaluated over 10 days. A digitoxigenin formulation for topical administration was prepared, and the in vitro release and in vivo wound healing effect were investigated; (3) Results: The expression of procollagen in human dermal fibroblast was significantly increased with the exposure to 0.1 nM digitoxigenin. Topical application of digitoxigenin in olive oil or alginate solution for three days significantly decreased the wound open in rats. Similarly, topical administration of the developed digitoxigenin formulation for three days also significantly increased wound healing. No wound healing effects were observed at days 7 and 10 after wounding when digitoxigenin was not applied; and, (4) Conclusions: It was possible to deliver digitoxigenin using the developed formulation. However, the wound healing effect of digitoxigenin and its mechanisms need to be further investigated in future studies. |
| first_indexed | 2024-03-12T10:15:34Z |
| format | Article |
| id | doaj.art-279f85f6f1ff4405a06825688228126e |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-12T10:15:34Z |
| publishDate | 2018-03-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-279f85f6f1ff4405a06825688228126e2023-09-02T10:28:37ZengMDPI AGBioengineering2306-53542018-03-01512110.3390/bioengineering5010021bioengineering5010021Topical Digitoxigenin for Wound Healing: A Feasibility StudyXinchi Feng0Cuifen Wang1Yunhui Xu2Joel Turley3Zijian Xie4Sandrine V. Pierre5Jinsong Hao6Department of Pharmaceutical Science and Research, School of Pharmacy, Marshall University, One John Marshall Drive, Huntington, WV 25755, USADepartment of Pharmaceutical Science and Research, School of Pharmacy, Marshall University, One John Marshall Drive, Huntington, WV 25755, USAMarshall Institute for Interdisciplinary Research, Marshall University, Huntington, WV 25703, USADepartment of Pharmaceutical Science and Research, School of Pharmacy, Marshall University, One John Marshall Drive, Huntington, WV 25755, USAMarshall Institute for Interdisciplinary Research, Marshall University, Huntington, WV 25703, USAMarshall Institute for Interdisciplinary Research, Marshall University, Huntington, WV 25703, USADepartment of Pharmaceutical Science and Research, School of Pharmacy, Marshall University, One John Marshall Drive, Huntington, WV 25755, USA(1) Background: Cardiotonic steroids have been found to stimulate collagen synthesis and might be potential wound healing therapeutics. The objective of this study was to evaluate the feasibility of digitoxigenin and its topical formulation for wound healing; (2) Methods: In the in vitro study, the human dermal fibroblast cells were treated with digitoxigenin and collagen synthesis was assessed. In the in vivo study, digitoxigenin was applied to excisional full-thickness wounds in rats immediately after wounding and remained for three days, and wound open was evaluated over 10 days. A digitoxigenin formulation for topical administration was prepared, and the in vitro release and in vivo wound healing effect were investigated; (3) Results: The expression of procollagen in human dermal fibroblast was significantly increased with the exposure to 0.1 nM digitoxigenin. Topical application of digitoxigenin in olive oil or alginate solution for three days significantly decreased the wound open in rats. Similarly, topical administration of the developed digitoxigenin formulation for three days also significantly increased wound healing. No wound healing effects were observed at days 7 and 10 after wounding when digitoxigenin was not applied; and, (4) Conclusions: It was possible to deliver digitoxigenin using the developed formulation. However, the wound healing effect of digitoxigenin and its mechanisms need to be further investigated in future studies.http://www.mdpi.com/2306-5354/5/1/21digitoxigeninwound healingfull-thickness excision woundhuman dermal fibroblastalginate |
| spellingShingle | Xinchi Feng Cuifen Wang Yunhui Xu Joel Turley Zijian Xie Sandrine V. Pierre Jinsong Hao Topical Digitoxigenin for Wound Healing: A Feasibility Study Bioengineering digitoxigenin wound healing full-thickness excision wound human dermal fibroblast alginate |
| title | Topical Digitoxigenin for Wound Healing: A Feasibility Study |
| title_full | Topical Digitoxigenin for Wound Healing: A Feasibility Study |
| title_fullStr | Topical Digitoxigenin for Wound Healing: A Feasibility Study |
| title_full_unstemmed | Topical Digitoxigenin for Wound Healing: A Feasibility Study |
| title_short | Topical Digitoxigenin for Wound Healing: A Feasibility Study |
| title_sort | topical digitoxigenin for wound healing a feasibility study |
| topic | digitoxigenin wound healing full-thickness excision wound human dermal fibroblast alginate |
| url | http://www.mdpi.com/2306-5354/5/1/21 |
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