The Novel Competing Endogenous Long Noncoding RNA SM2 Regulates Gonadotropin Secretion in the Hu Sheep Anterior Pituitary by Targeting the Oar-miR-16b/TGF-β/SMAD2 Signaling Pathway
Pituitary gonadotropins play a pivotal role in reproduction. Long noncoding RNAs (lncRNAs) have been identified as important regulators in the hypothalamic–pituitary–ovarian (HPO) axis associated with reproduction. However, the contributions of lncRNAs to pituitary gonadotropin secretion remain larg...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-03-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/11/6/985 |
| _version_ | 1797472356206116864 |
|---|---|
| author | Zhen Wan Hua Yang Peiyong Chen Zhibo Wang Yu Cai Xiaolei Yao Feng Wang Yanli Zhang |
| author_facet | Zhen Wan Hua Yang Peiyong Chen Zhibo Wang Yu Cai Xiaolei Yao Feng Wang Yanli Zhang |
| author_sort | Zhen Wan |
| collection | DOAJ |
| description | Pituitary gonadotropins play a pivotal role in reproduction. Long noncoding RNAs (lncRNAs) have been identified as important regulators in the hypothalamic–pituitary–ovarian (HPO) axis associated with reproduction. However, the contributions of lncRNAs to pituitary gonadotropin secretion remain largely unknown. Therefore, this work was performed to uncover the functional mechanisms of the novel lncRNA TCONS_00083279 (lncRNA SM2) and its potential targeting pathway oar-miR-16b/TGF-beta/SMAD2, which is associated with gonadotropin secretion in sheep pituitary cells. In the present study, the lncRNA SM2 showed high expression levels in the sheep pituitary gland, and it was located in both the nucleus and the cytoplasm of pituitary cells. lncRNA SM2 knockdown inhibited pituitary cell proliferation and FSH and LH secretion. The function of the lncRNA SM2 was sponged by oar-miR-16b, and this regulated the growth and gonadotropin secretion of pituitary cells by modulating SMAD2, as shown by the dual-luciferase reporter assay. FSH and LH levels were both upregulated by SMAD2 overexpression. Moreover, the levels of the lncRNA SM2, SMAD2 and TGFR1, as well as FSH and LH, in sheep pituitary cells increased significantly under gonadotropin-releasing hormone (GnRH) stimulation (<i>p</i> < 0.05). This work illustrates that the lncRNA SM2 regulates gonadotropin secretion in the Hu sheep anterior pituitary by targeting the oar-miR-16b/TGF-β/SMAD2 signaling pathway, providing a valuable resource for understanding the molecular mechanisms underlying sheep reproduction. |
| first_indexed | 2024-03-09T20:00:41Z |
| format | Article |
| id | doaj.art-279fd9f1644f40db989222f78cd7d0e4 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T20:00:41Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-279fd9f1644f40db989222f78cd7d0e42023-11-24T00:44:14ZengMDPI AGCells2073-44092022-03-0111698510.3390/cells11060985The Novel Competing Endogenous Long Noncoding RNA SM2 Regulates Gonadotropin Secretion in the Hu Sheep Anterior Pituitary by Targeting the Oar-miR-16b/TGF-β/SMAD2 Signaling PathwayZhen Wan0Hua Yang1Peiyong Chen2Zhibo Wang3Yu Cai4Xiaolei Yao5Feng Wang6Yanli Zhang7Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, ChinaJiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, ChinaJiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, ChinaJiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, ChinaJiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, ChinaJiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, ChinaJiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, ChinaJiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, ChinaPituitary gonadotropins play a pivotal role in reproduction. Long noncoding RNAs (lncRNAs) have been identified as important regulators in the hypothalamic–pituitary–ovarian (HPO) axis associated with reproduction. However, the contributions of lncRNAs to pituitary gonadotropin secretion remain largely unknown. Therefore, this work was performed to uncover the functional mechanisms of the novel lncRNA TCONS_00083279 (lncRNA SM2) and its potential targeting pathway oar-miR-16b/TGF-beta/SMAD2, which is associated with gonadotropin secretion in sheep pituitary cells. In the present study, the lncRNA SM2 showed high expression levels in the sheep pituitary gland, and it was located in both the nucleus and the cytoplasm of pituitary cells. lncRNA SM2 knockdown inhibited pituitary cell proliferation and FSH and LH secretion. The function of the lncRNA SM2 was sponged by oar-miR-16b, and this regulated the growth and gonadotropin secretion of pituitary cells by modulating SMAD2, as shown by the dual-luciferase reporter assay. FSH and LH levels were both upregulated by SMAD2 overexpression. Moreover, the levels of the lncRNA SM2, SMAD2 and TGFR1, as well as FSH and LH, in sheep pituitary cells increased significantly under gonadotropin-releasing hormone (GnRH) stimulation (<i>p</i> < 0.05). This work illustrates that the lncRNA SM2 regulates gonadotropin secretion in the Hu sheep anterior pituitary by targeting the oar-miR-16b/TGF-β/SMAD2 signaling pathway, providing a valuable resource for understanding the molecular mechanisms underlying sheep reproduction.https://www.mdpi.com/2073-4409/11/6/985pituitary cellslncRNA SM2TGF-β/SMAD2oar-miR-16bgonadotropin |
| spellingShingle | Zhen Wan Hua Yang Peiyong Chen Zhibo Wang Yu Cai Xiaolei Yao Feng Wang Yanli Zhang The Novel Competing Endogenous Long Noncoding RNA SM2 Regulates Gonadotropin Secretion in the Hu Sheep Anterior Pituitary by Targeting the Oar-miR-16b/TGF-β/SMAD2 Signaling Pathway Cells pituitary cells lncRNA SM2 TGF-β/SMAD2 oar-miR-16b gonadotropin |
| title | The Novel Competing Endogenous Long Noncoding RNA SM2 Regulates Gonadotropin Secretion in the Hu Sheep Anterior Pituitary by Targeting the Oar-miR-16b/TGF-β/SMAD2 Signaling Pathway |
| title_full | The Novel Competing Endogenous Long Noncoding RNA SM2 Regulates Gonadotropin Secretion in the Hu Sheep Anterior Pituitary by Targeting the Oar-miR-16b/TGF-β/SMAD2 Signaling Pathway |
| title_fullStr | The Novel Competing Endogenous Long Noncoding RNA SM2 Regulates Gonadotropin Secretion in the Hu Sheep Anterior Pituitary by Targeting the Oar-miR-16b/TGF-β/SMAD2 Signaling Pathway |
| title_full_unstemmed | The Novel Competing Endogenous Long Noncoding RNA SM2 Regulates Gonadotropin Secretion in the Hu Sheep Anterior Pituitary by Targeting the Oar-miR-16b/TGF-β/SMAD2 Signaling Pathway |
| title_short | The Novel Competing Endogenous Long Noncoding RNA SM2 Regulates Gonadotropin Secretion in the Hu Sheep Anterior Pituitary by Targeting the Oar-miR-16b/TGF-β/SMAD2 Signaling Pathway |
| title_sort | novel competing endogenous long noncoding rna sm2 regulates gonadotropin secretion in the hu sheep anterior pituitary by targeting the oar mir 16b tgf β smad2 signaling pathway |
| topic | pituitary cells lncRNA SM2 TGF-β/SMAD2 oar-miR-16b gonadotropin |
| url | https://www.mdpi.com/2073-4409/11/6/985 |
| work_keys_str_mv | AT zhenwan thenovelcompetingendogenouslongnoncodingrnasm2regulatesgonadotropinsecretioninthehusheepanteriorpituitarybytargetingtheoarmir16btgfbsmad2signalingpathway AT huayang thenovelcompetingendogenouslongnoncodingrnasm2regulatesgonadotropinsecretioninthehusheepanteriorpituitarybytargetingtheoarmir16btgfbsmad2signalingpathway AT peiyongchen thenovelcompetingendogenouslongnoncodingrnasm2regulatesgonadotropinsecretioninthehusheepanteriorpituitarybytargetingtheoarmir16btgfbsmad2signalingpathway AT zhibowang thenovelcompetingendogenouslongnoncodingrnasm2regulatesgonadotropinsecretioninthehusheepanteriorpituitarybytargetingtheoarmir16btgfbsmad2signalingpathway AT yucai thenovelcompetingendogenouslongnoncodingrnasm2regulatesgonadotropinsecretioninthehusheepanteriorpituitarybytargetingtheoarmir16btgfbsmad2signalingpathway AT xiaoleiyao thenovelcompetingendogenouslongnoncodingrnasm2regulatesgonadotropinsecretioninthehusheepanteriorpituitarybytargetingtheoarmir16btgfbsmad2signalingpathway AT fengwang thenovelcompetingendogenouslongnoncodingrnasm2regulatesgonadotropinsecretioninthehusheepanteriorpituitarybytargetingtheoarmir16btgfbsmad2signalingpathway AT yanlizhang thenovelcompetingendogenouslongnoncodingrnasm2regulatesgonadotropinsecretioninthehusheepanteriorpituitarybytargetingtheoarmir16btgfbsmad2signalingpathway AT zhenwan novelcompetingendogenouslongnoncodingrnasm2regulatesgonadotropinsecretioninthehusheepanteriorpituitarybytargetingtheoarmir16btgfbsmad2signalingpathway AT huayang novelcompetingendogenouslongnoncodingrnasm2regulatesgonadotropinsecretioninthehusheepanteriorpituitarybytargetingtheoarmir16btgfbsmad2signalingpathway AT peiyongchen novelcompetingendogenouslongnoncodingrnasm2regulatesgonadotropinsecretioninthehusheepanteriorpituitarybytargetingtheoarmir16btgfbsmad2signalingpathway AT zhibowang novelcompetingendogenouslongnoncodingrnasm2regulatesgonadotropinsecretioninthehusheepanteriorpituitarybytargetingtheoarmir16btgfbsmad2signalingpathway AT yucai novelcompetingendogenouslongnoncodingrnasm2regulatesgonadotropinsecretioninthehusheepanteriorpituitarybytargetingtheoarmir16btgfbsmad2signalingpathway AT xiaoleiyao novelcompetingendogenouslongnoncodingrnasm2regulatesgonadotropinsecretioninthehusheepanteriorpituitarybytargetingtheoarmir16btgfbsmad2signalingpathway AT fengwang novelcompetingendogenouslongnoncodingrnasm2regulatesgonadotropinsecretioninthehusheepanteriorpituitarybytargetingtheoarmir16btgfbsmad2signalingpathway AT yanlizhang novelcompetingendogenouslongnoncodingrnasm2regulatesgonadotropinsecretioninthehusheepanteriorpituitarybytargetingtheoarmir16btgfbsmad2signalingpathway |