Peptide-Conjugated Quantum Dots Act as the Target Marker for Human Pancreatic Carcinoma Cells
Background/Aims: In the present study, we describe a novel and straightforward approach to produce a cyclic- arginine-glycine-aspartic (RGD)-peptide-conjugated quantum dot (QD) probe as an ideal target tumor biomarker. Due to its specific structure, the probe can be used for targeted imaging of panc...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2016-03-01
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| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/443062 |
| _version_ | 1818034792965341184 |
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| author | Shuang-ling Li Jing Yang Xiao-fei Lei Jian-na Zhang Hong-li Yang Kun Li Chang-qing Xu |
| author_facet | Shuang-ling Li Jing Yang Xiao-fei Lei Jian-na Zhang Hong-li Yang Kun Li Chang-qing Xu |
| author_sort | Shuang-ling Li |
| collection | DOAJ |
| description | Background/Aims: In the present study, we describe a novel and straightforward approach to produce a cyclic- arginine-glycine-aspartic (RGD)-peptide-conjugated quantum dot (QD) probe as an ideal target tumor biomarker. Due to its specific structure, the probe can be used for targeted imaging of pancreatic carcinoma cells. Methods: Pancreatic carcinoma cells were routinely cultured and marked with QD-RGD probe. The QD-RGD probe on the fluorescence-labeled cancer cell was observed by fluorescence microscopy and laser confocal microscopy. Cancer cell viability was detected by MTT assay after culturing with QD-RGD probe. Results: Fluorescence microscopy and laser confocal microscopy displayed that 10nmol/L QD-RGD probe was able to effectively mark pancreatic carcinoma cells. In comparison with organic dyes and fluorescent proteins, the quantum dot-RGD probe had unique optical and electronic properties. Conclusion: QD-RGD probe has a low cytotoxicity with an excellent optical property and biocompatibility. These findings support further evaluation of QD-RGD probes for the early detection of pancreatic cancer. |
| first_indexed | 2024-12-10T06:44:48Z |
| format | Article |
| id | doaj.art-27a1a611d4db4f18984b6f79de94f8b2 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-10T06:44:48Z |
| publishDate | 2016-03-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-27a1a611d4db4f18984b6f79de94f8b22022-12-22T01:58:41ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782016-03-013831121112810.1159/000443062443062Peptide-Conjugated Quantum Dots Act as the Target Marker for Human Pancreatic Carcinoma CellsShuang-ling LiJing YangXiao-fei LeiJian-na ZhangHong-li YangKun LiChang-qing XuBackground/Aims: In the present study, we describe a novel and straightforward approach to produce a cyclic- arginine-glycine-aspartic (RGD)-peptide-conjugated quantum dot (QD) probe as an ideal target tumor biomarker. Due to its specific structure, the probe can be used for targeted imaging of pancreatic carcinoma cells. Methods: Pancreatic carcinoma cells were routinely cultured and marked with QD-RGD probe. The QD-RGD probe on the fluorescence-labeled cancer cell was observed by fluorescence microscopy and laser confocal microscopy. Cancer cell viability was detected by MTT assay after culturing with QD-RGD probe. Results: Fluorescence microscopy and laser confocal microscopy displayed that 10nmol/L QD-RGD probe was able to effectively mark pancreatic carcinoma cells. In comparison with organic dyes and fluorescent proteins, the quantum dot-RGD probe had unique optical and electronic properties. Conclusion: QD-RGD probe has a low cytotoxicity with an excellent optical property and biocompatibility. These findings support further evaluation of QD-RGD probes for the early detection of pancreatic cancer.http://www.karger.com/Article/FullText/443062NanotechnologyQuantum dotsCyclic RGDTargeted markingBiocompatibility |
| spellingShingle | Shuang-ling Li Jing Yang Xiao-fei Lei Jian-na Zhang Hong-li Yang Kun Li Chang-qing Xu Peptide-Conjugated Quantum Dots Act as the Target Marker for Human Pancreatic Carcinoma Cells Cellular Physiology and Biochemistry Nanotechnology Quantum dots Cyclic RGD Targeted marking Biocompatibility |
| title | Peptide-Conjugated Quantum Dots Act as the Target Marker for Human Pancreatic Carcinoma Cells |
| title_full | Peptide-Conjugated Quantum Dots Act as the Target Marker for Human Pancreatic Carcinoma Cells |
| title_fullStr | Peptide-Conjugated Quantum Dots Act as the Target Marker for Human Pancreatic Carcinoma Cells |
| title_full_unstemmed | Peptide-Conjugated Quantum Dots Act as the Target Marker for Human Pancreatic Carcinoma Cells |
| title_short | Peptide-Conjugated Quantum Dots Act as the Target Marker for Human Pancreatic Carcinoma Cells |
| title_sort | peptide conjugated quantum dots act as the target marker for human pancreatic carcinoma cells |
| topic | Nanotechnology Quantum dots Cyclic RGD Targeted marking Biocompatibility |
| url | http://www.karger.com/Article/FullText/443062 |
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