Stability of Dry and Liquid <i>Metschnikowia pulcherrima</i> Formulations for Biocontrol Applications against Apple Postharvest Diseases
The yeast <i>Metschnikowia pulcherrima</i> is frequently isolated from environmental samples and has often been reported to exhibit strong antagonistic activity against plant pathogens. In order to assess the potential of this species for its development into a plant protection product,...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/7/11/459 |
| _version_ | 1797510045953425408 |
|---|---|
| author | Andreas Bühlmann Sandrine Kammerecker Laurin Müller Maja Hilber-Bodmer Sarah Perren Florian M. Freimoser |
| author_facet | Andreas Bühlmann Sandrine Kammerecker Laurin Müller Maja Hilber-Bodmer Sarah Perren Florian M. Freimoser |
| author_sort | Andreas Bühlmann |
| collection | DOAJ |
| description | The yeast <i>Metschnikowia pulcherrima</i> is frequently isolated from environmental samples and has often been reported to exhibit strong antagonistic activity against plant pathogens. In order to assess the potential of this species for its development into a plant protection product, the survival during formulation and storage were quantified and field efficacy was assessed over a period of five years. Freeze dried and liquid <i>M. pulcherrima</i> formulations (i.e., with skim milk powder (SMP), sucrose, glycerol, xanthan, without additives) were prepared and the number of viable cells was quantified during storage at different temperatures. Field trials against apple postharvest diseases (<i>Neofabreae</i>) were performed with different dry formulations. <i>M. pulcherrima</i> proved exceptionally stable for many months and even years. Five years of field trials with the yeast revealed variable effects, but reduced <i>Neofabreae</i> infections of stored apples were observed in some years. <i>M. pulcherrima</i> applications after prior fungicide treatments repeatedly showed an additive effect as compared to the fungicide treatments alone. In summary, <i>M. pulcherrima</i> exhibited highly advantageous storage properties and encouraging activity against apple postharvest rots. Further studies to identify the factors responsible for antagonistic activity in the field and survival during storage are expected to lay the foundation for the future development of a plant protection product. |
| first_indexed | 2024-03-10T05:27:19Z |
| format | Article |
| id | doaj.art-27a2344a9f5c473ea998fdc675add47b |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T05:27:19Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-27a2344a9f5c473ea998fdc675add47b2023-11-22T23:33:58ZengMDPI AGHorticulturae2311-75242021-11-0171145910.3390/horticulturae7110459Stability of Dry and Liquid <i>Metschnikowia pulcherrima</i> Formulations for Biocontrol Applications against Apple Postharvest DiseasesAndreas Bühlmann0Sandrine Kammerecker1Laurin Müller2Maja Hilber-Bodmer3Sarah Perren4Florian M. Freimoser5Agroscope, Competence Division Plants and Plant Products, Müller-Thurgau-Strasse 29, 8820 Wädenswil, SwitzerlandAgroscope, Research Division Plant Protection, Müller-Thurgau-Strasse 29, 8820 Wädenswil, SwitzerlandAgroscope, Research Division Plant Protection, Müller-Thurgau-Strasse 29, 8820 Wädenswil, SwitzerlandAgroscope, Research Division Plant Protection, Müller-Thurgau-Strasse 29, 8820 Wädenswil, SwitzerlandAgroscope, Competence Division Plants and Plant Products, Müller-Thurgau-Strasse 29, 8820 Wädenswil, SwitzerlandAgroscope, Research Division Plant Protection, Müller-Thurgau-Strasse 29, 8820 Wädenswil, SwitzerlandThe yeast <i>Metschnikowia pulcherrima</i> is frequently isolated from environmental samples and has often been reported to exhibit strong antagonistic activity against plant pathogens. In order to assess the potential of this species for its development into a plant protection product, the survival during formulation and storage were quantified and field efficacy was assessed over a period of five years. Freeze dried and liquid <i>M. pulcherrima</i> formulations (i.e., with skim milk powder (SMP), sucrose, glycerol, xanthan, without additives) were prepared and the number of viable cells was quantified during storage at different temperatures. Field trials against apple postharvest diseases (<i>Neofabreae</i>) were performed with different dry formulations. <i>M. pulcherrima</i> proved exceptionally stable for many months and even years. Five years of field trials with the yeast revealed variable effects, but reduced <i>Neofabreae</i> infections of stored apples were observed in some years. <i>M. pulcherrima</i> applications after prior fungicide treatments repeatedly showed an additive effect as compared to the fungicide treatments alone. In summary, <i>M. pulcherrima</i> exhibited highly advantageous storage properties and encouraging activity against apple postharvest rots. Further studies to identify the factors responsible for antagonistic activity in the field and survival during storage are expected to lay the foundation for the future development of a plant protection product.https://www.mdpi.com/2311-7524/7/11/459biocontrolfield trialformulation<i>Metschnikowia</i><i>Neofabraea</i>postharvest disease |
| spellingShingle | Andreas Bühlmann Sandrine Kammerecker Laurin Müller Maja Hilber-Bodmer Sarah Perren Florian M. Freimoser Stability of Dry and Liquid <i>Metschnikowia pulcherrima</i> Formulations for Biocontrol Applications against Apple Postharvest Diseases Horticulturae biocontrol field trial formulation <i>Metschnikowia</i> <i>Neofabraea</i> postharvest disease |
| title | Stability of Dry and Liquid <i>Metschnikowia pulcherrima</i> Formulations for Biocontrol Applications against Apple Postharvest Diseases |
| title_full | Stability of Dry and Liquid <i>Metschnikowia pulcherrima</i> Formulations for Biocontrol Applications against Apple Postharvest Diseases |
| title_fullStr | Stability of Dry and Liquid <i>Metschnikowia pulcherrima</i> Formulations for Biocontrol Applications against Apple Postharvest Diseases |
| title_full_unstemmed | Stability of Dry and Liquid <i>Metschnikowia pulcherrima</i> Formulations for Biocontrol Applications against Apple Postharvest Diseases |
| title_short | Stability of Dry and Liquid <i>Metschnikowia pulcherrima</i> Formulations for Biocontrol Applications against Apple Postharvest Diseases |
| title_sort | stability of dry and liquid i metschnikowia pulcherrima i formulations for biocontrol applications against apple postharvest diseases |
| topic | biocontrol field trial formulation <i>Metschnikowia</i> <i>Neofabraea</i> postharvest disease |
| url | https://www.mdpi.com/2311-7524/7/11/459 |
| work_keys_str_mv | AT andreasbuhlmann stabilityofdryandliquidimetschnikowiapulcherrimaiformulationsforbiocontrolapplicationsagainstapplepostharvestdiseases AT sandrinekammerecker stabilityofdryandliquidimetschnikowiapulcherrimaiformulationsforbiocontrolapplicationsagainstapplepostharvestdiseases AT laurinmuller stabilityofdryandliquidimetschnikowiapulcherrimaiformulationsforbiocontrolapplicationsagainstapplepostharvestdiseases AT majahilberbodmer stabilityofdryandliquidimetschnikowiapulcherrimaiformulationsforbiocontrolapplicationsagainstapplepostharvestdiseases AT sarahperren stabilityofdryandliquidimetschnikowiapulcherrimaiformulationsforbiocontrolapplicationsagainstapplepostharvestdiseases AT florianmfreimoser stabilityofdryandliquidimetschnikowiapulcherrimaiformulationsforbiocontrolapplicationsagainstapplepostharvestdiseases |