Low expression of lncRNA-GAS5 is implicated in human primary varicose great saphenous veins.
The cellular mechanisms of primary varicose great saphenous veins (GSVs) involve inflammation, apoptosis, and proliferation of local cells and extracellular matrix degradation. Long non-coding RNAs (lncRNAs) play important roles in these cellular processes; however, which and how lncRNAs related to...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4373870?pdf=render |
| _version_ | 1818285744423174144 |
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| author | Li Li Xiang Li Erlinda The Li-Jie Wang Tian-You Yuan Shi-Yi Wang Jing Feng Jing Wang Yuan Liu Ya-Han Wu Xiu-E Ma Jin Ge Ying-Yu Cui Xiao-Yan Jiang |
| author_facet | Li Li Xiang Li Erlinda The Li-Jie Wang Tian-You Yuan Shi-Yi Wang Jing Feng Jing Wang Yuan Liu Ya-Han Wu Xiu-E Ma Jin Ge Ying-Yu Cui Xiao-Yan Jiang |
| author_sort | Li Li |
| collection | DOAJ |
| description | The cellular mechanisms of primary varicose great saphenous veins (GSVs) involve inflammation, apoptosis, and proliferation of local cells and extracellular matrix degradation. Long non-coding RNAs (lncRNAs) play important roles in these cellular processes; however, which and how lncRNAs related to these mechanisms take effect on GSVs remain unclear. By screening lncRNAs that might experience changes in GSV varicosities, we selected the lower expressed lncRNA-GAS5 (growth arrest specific transcript 5) for functional assessments. Silencing of lncRNA-GAS5 promoted cell proliferation and migration, and cell cycle of the human saphenous vein smooth muscle cells (HSVSMCs), whereas overexpressing it inhibited these cellular behaviors and reduced apoptosis of HSVSMCs. RNA pull-down experiment revealed a direct bind of lncRNA-GAS5 to a Ca2+-dependent RNA-binding protein, Annexin A2. Further experiments showed that silencing of Annexin A2 reduced the HSVSMCs proliferation and vice versa. In the context of lncRNA-GAS5 knockdown, silencing of Annexin A2 reduced the proliferation of HSVSMCs while overexpression of Annexin A2 increased the proliferation. Thus, the low expression of lncRNA-GAS5 may facilitate HSVSMCs proliferation and migration through Annexin A2 and thereby the pathogenesis of GSV varicosities. |
| first_indexed | 2024-12-13T01:13:34Z |
| format | Article |
| id | doaj.art-27a284cee32844d68bd21ad094376363 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T01:13:34Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-27a284cee32844d68bd21ad0943763632022-12-22T00:04:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01103e012055010.1371/journal.pone.0120550Low expression of lncRNA-GAS5 is implicated in human primary varicose great saphenous veins.Li LiXiang LiErlinda TheLi-Jie WangTian-You YuanShi-Yi WangJing FengJing WangYuan LiuYa-Han WuXiu-E MaJin GeYing-Yu CuiXiao-Yan JiangThe cellular mechanisms of primary varicose great saphenous veins (GSVs) involve inflammation, apoptosis, and proliferation of local cells and extracellular matrix degradation. Long non-coding RNAs (lncRNAs) play important roles in these cellular processes; however, which and how lncRNAs related to these mechanisms take effect on GSVs remain unclear. By screening lncRNAs that might experience changes in GSV varicosities, we selected the lower expressed lncRNA-GAS5 (growth arrest specific transcript 5) for functional assessments. Silencing of lncRNA-GAS5 promoted cell proliferation and migration, and cell cycle of the human saphenous vein smooth muscle cells (HSVSMCs), whereas overexpressing it inhibited these cellular behaviors and reduced apoptosis of HSVSMCs. RNA pull-down experiment revealed a direct bind of lncRNA-GAS5 to a Ca2+-dependent RNA-binding protein, Annexin A2. Further experiments showed that silencing of Annexin A2 reduced the HSVSMCs proliferation and vice versa. In the context of lncRNA-GAS5 knockdown, silencing of Annexin A2 reduced the proliferation of HSVSMCs while overexpression of Annexin A2 increased the proliferation. Thus, the low expression of lncRNA-GAS5 may facilitate HSVSMCs proliferation and migration through Annexin A2 and thereby the pathogenesis of GSV varicosities.http://europepmc.org/articles/PMC4373870?pdf=render |
| spellingShingle | Li Li Xiang Li Erlinda The Li-Jie Wang Tian-You Yuan Shi-Yi Wang Jing Feng Jing Wang Yuan Liu Ya-Han Wu Xiu-E Ma Jin Ge Ying-Yu Cui Xiao-Yan Jiang Low expression of lncRNA-GAS5 is implicated in human primary varicose great saphenous veins. PLoS ONE |
| title | Low expression of lncRNA-GAS5 is implicated in human primary varicose great saphenous veins. |
| title_full | Low expression of lncRNA-GAS5 is implicated in human primary varicose great saphenous veins. |
| title_fullStr | Low expression of lncRNA-GAS5 is implicated in human primary varicose great saphenous veins. |
| title_full_unstemmed | Low expression of lncRNA-GAS5 is implicated in human primary varicose great saphenous veins. |
| title_short | Low expression of lncRNA-GAS5 is implicated in human primary varicose great saphenous veins. |
| title_sort | low expression of lncrna gas5 is implicated in human primary varicose great saphenous veins |
| url | http://europepmc.org/articles/PMC4373870?pdf=render |
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