Biosynthesis and Properties of a P(3HB-<i>co</i>-3HV-<i>co</i>-4HV) Produced by <i>Cupriavidus necator</i> B-10646
Synthesis of P(3HB-<i>co</i>-3HV-<i>co</i>-4HV) copolymers by the wild-type strain <i>Cupriavidus necator</i> B-10646 on fructose or sodium butyrate as the main C-substrate with the addition of γ-valerolactone as a precursor of 3HV and 4HV monomers was studied. Ba...
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2022-10-01
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author | Natalia O. Zhila Kristina Yu. Sapozhnikova Evgeniy G. Kiselev Ivan V. Nemtsev Anna V. Lukyanenko Ekaterina I. Shishatskaya Tatiana G. Volova |
author_facet | Natalia O. Zhila Kristina Yu. Sapozhnikova Evgeniy G. Kiselev Ivan V. Nemtsev Anna V. Lukyanenko Ekaterina I. Shishatskaya Tatiana G. Volova |
author_sort | Natalia O. Zhila |
collection | DOAJ |
description | Synthesis of P(3HB-<i>co</i>-3HV-<i>co</i>-4HV) copolymers by the wild-type strain <i>Cupriavidus necator</i> B-10646 on fructose or sodium butyrate as the main C-substrate with the addition of γ-valerolactone as a precursor of 3HV and 4HV monomers was studied. Bacterial cells were cultivated in the modes that enabled production of a series of copolymers with molar fractions of 3HV (from 7.3 to 23.4 mol.%) and 4HV (from 1.9 to 4.7 mol.%) with bacterial biomass concentration (8.2 ± 0.2 g/L) and PHA content (80 ± 2%). Using HPLC, DTA, DSC, X-Ray, SEM, and AFM, the physicochemical properties of copolymers and films prepared from them have been investigated as dependent on proportions of monomers. Copolymers are characterized by a reduced degree of crystallinity (C<sub>x</sub> 38–49%) molecular weight characteristics M<sub>n</sub> (45–87 kDa), and M<sub>w</sub> (201–248 kDa) compared with P(3HB). The properties of the films surface of various composition including the porosity and surface roughness were studied. Most of the samples showed a decrease in the average pore area and an increase in their number with a total increase in 3HV and 4HV monomers. The results allow scaling up the productive synthesis of P(3HB-<i>co</i>-3HV-<i>co</i>-4HV) copolymers using <i>Cupriavidus necator</i> B-10646. |
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language | English |
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spelling | doaj.art-27a4502a189440a583d148413ba56a7f2023-11-23T21:36:23ZengMDPI AGPolymers2073-43602022-10-011419422610.3390/polym14194226Biosynthesis and Properties of a P(3HB-<i>co</i>-3HV-<i>co</i>-4HV) Produced by <i>Cupriavidus necator</i> B-10646Natalia O. Zhila0Kristina Yu. Sapozhnikova1Evgeniy G. Kiselev2Ivan V. Nemtsev3Anna V. Lukyanenko4Ekaterina I. Shishatskaya5Tatiana G. Volova6Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk 660036, RussiaInstitute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk 660036, RussiaInstitute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk 660036, RussiaBasic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk 660041, RussiaBasic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk 660041, RussiaInstitute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk 660036, RussiaInstitute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk 660036, RussiaSynthesis of P(3HB-<i>co</i>-3HV-<i>co</i>-4HV) copolymers by the wild-type strain <i>Cupriavidus necator</i> B-10646 on fructose or sodium butyrate as the main C-substrate with the addition of γ-valerolactone as a precursor of 3HV and 4HV monomers was studied. Bacterial cells were cultivated in the modes that enabled production of a series of copolymers with molar fractions of 3HV (from 7.3 to 23.4 mol.%) and 4HV (from 1.9 to 4.7 mol.%) with bacterial biomass concentration (8.2 ± 0.2 g/L) and PHA content (80 ± 2%). Using HPLC, DTA, DSC, X-Ray, SEM, and AFM, the physicochemical properties of copolymers and films prepared from them have been investigated as dependent on proportions of monomers. Copolymers are characterized by a reduced degree of crystallinity (C<sub>x</sub> 38–49%) molecular weight characteristics M<sub>n</sub> (45–87 kDa), and M<sub>w</sub> (201–248 kDa) compared with P(3HB). The properties of the films surface of various composition including the porosity and surface roughness were studied. Most of the samples showed a decrease in the average pore area and an increase in their number with a total increase in 3HV and 4HV monomers. The results allow scaling up the productive synthesis of P(3HB-<i>co</i>-3HV-<i>co</i>-4HV) copolymers using <i>Cupriavidus necator</i> B-10646.https://www.mdpi.com/2073-4360/14/19/4226<i>Cupriavidus necator</i> B-10646γ-valerolactonebiomass concentrationcontent and composition of copolymersP(3HB-<i>co</i>-3HV-<i>co</i>-4HV)physicochemical properties |
spellingShingle | Natalia O. Zhila Kristina Yu. Sapozhnikova Evgeniy G. Kiselev Ivan V. Nemtsev Anna V. Lukyanenko Ekaterina I. Shishatskaya Tatiana G. Volova Biosynthesis and Properties of a P(3HB-<i>co</i>-3HV-<i>co</i>-4HV) Produced by <i>Cupriavidus necator</i> B-10646 Polymers <i>Cupriavidus necator</i> B-10646 γ-valerolactone biomass concentration content and composition of copolymers P(3HB-<i>co</i>-3HV-<i>co</i>-4HV) physicochemical properties |
title | Biosynthesis and Properties of a P(3HB-<i>co</i>-3HV-<i>co</i>-4HV) Produced by <i>Cupriavidus necator</i> B-10646 |
title_full | Biosynthesis and Properties of a P(3HB-<i>co</i>-3HV-<i>co</i>-4HV) Produced by <i>Cupriavidus necator</i> B-10646 |
title_fullStr | Biosynthesis and Properties of a P(3HB-<i>co</i>-3HV-<i>co</i>-4HV) Produced by <i>Cupriavidus necator</i> B-10646 |
title_full_unstemmed | Biosynthesis and Properties of a P(3HB-<i>co</i>-3HV-<i>co</i>-4HV) Produced by <i>Cupriavidus necator</i> B-10646 |
title_short | Biosynthesis and Properties of a P(3HB-<i>co</i>-3HV-<i>co</i>-4HV) Produced by <i>Cupriavidus necator</i> B-10646 |
title_sort | biosynthesis and properties of a p 3hb i co i 3hv i co i 4hv produced by i cupriavidus necator i b 10646 |
topic | <i>Cupriavidus necator</i> B-10646 γ-valerolactone biomass concentration content and composition of copolymers P(3HB-<i>co</i>-3HV-<i>co</i>-4HV) physicochemical properties |
url | https://www.mdpi.com/2073-4360/14/19/4226 |
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