<i>MtWOX2</i> and <i>MtWOX9-1</i> Effects on the Embryogenic Callus Transcriptome in <i>Medicago truncatula</i>
WOX family transcription factors are well-known regulators of plant development, controlling cell proliferation and differentiation in diverse organs and tissues. Several <i>WOX</i> genes have been shown to participate in regeneration processes which take place in plant cell cultures in...
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MDPI AG
2023-12-01
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| Series: | Plants |
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| author | Elizaveta Y. Krasnoperova Varvara E. Tvorogova Kirill V. Smirnov Elena P. Efremova Elina A. Potsenkovskaia Anastasia M. Artemiuk Zakhar S. Konstantinov Veronika Y. Simonova Anna V. Brynchikova Daria V. Yakovleva Daria B. Pavlova Ludmila A. Lutova |
| author_facet | Elizaveta Y. Krasnoperova Varvara E. Tvorogova Kirill V. Smirnov Elena P. Efremova Elina A. Potsenkovskaia Anastasia M. Artemiuk Zakhar S. Konstantinov Veronika Y. Simonova Anna V. Brynchikova Daria V. Yakovleva Daria B. Pavlova Ludmila A. Lutova |
| author_sort | Elizaveta Y. Krasnoperova |
| collection | DOAJ |
| description | WOX family transcription factors are well-known regulators of plant development, controlling cell proliferation and differentiation in diverse organs and tissues. Several <i>WOX</i> genes have been shown to participate in regeneration processes which take place in plant cell cultures in vitro, but the effects of most of them on tissue culture development have not been discovered yet. In this study, we evaluated the effects of <i>MtWOX2</i> gene overexpression on the embryogenic callus development and transcriptomic state in <i>Medicago truncatula</i>. According to our results, overexpression of <i>MtWOX2</i> leads to an increase in callus weight. Furthermore, transcriptomic changes in <i>MtWOX2</i> overexpressing calli are, to a large extent, opposite to the changes caused by overexpression of <i>MtWOX9-1</i>, a somatic embryogenesis stimulator. These results add new information about the mechanisms of interaction between different <i>WOX</i> genes and can be useful for the search of new regeneration regulators. |
| first_indexed | 2024-03-08T14:59:05Z |
| format | Article |
| id | doaj.art-27a52d104ba74832ac2e8cb3475af325 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-08T14:59:05Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-27a52d104ba74832ac2e8cb3475af3252024-01-10T15:06:17ZengMDPI AGPlants2223-77472023-12-0113110210.3390/plants13010102<i>MtWOX2</i> and <i>MtWOX9-1</i> Effects on the Embryogenic Callus Transcriptome in <i>Medicago truncatula</i>Elizaveta Y. Krasnoperova0Varvara E. Tvorogova1Kirill V. Smirnov2Elena P. Efremova3Elina A. Potsenkovskaia4Anastasia M. Artemiuk5Zakhar S. Konstantinov6Veronika Y. Simonova7Anna V. Brynchikova8Daria V. Yakovleva9Daria B. Pavlova10Ludmila A. Lutova11Department of Genetics and Biotechnology, Saint Petersburg State University, 7/9 Universitetskaya Emb, 199034 St. Petersburg, RussiaDepartment of Genetics and Biotechnology, Saint Petersburg State University, 7/9 Universitetskaya Emb, 199034 St. Petersburg, RussiaAll-Russia Research Institute for Agricultural Microbiology, Podbelsky Chausse 3, Pushkin, 196608 St. Petersburg, RussiaDepartment of Genetics and Biotechnology, Saint Petersburg State University, 7/9 Universitetskaya Emb, 199034 St. Petersburg, RussiaDepartment of Genetics and Biotechnology, Saint Petersburg State University, 7/9 Universitetskaya Emb, 199034 St. Petersburg, RussiaDepartment of Genetics and Biotechnology, Saint Petersburg State University, 7/9 Universitetskaya Emb, 199034 St. Petersburg, RussiaPlant Biology and Biotechnology Department, Sirius University of Science and Technology, 1 Olympic Avenue, 354340 Sochi, RussiaPlant Biology and Biotechnology Department, Sirius University of Science and Technology, 1 Olympic Avenue, 354340 Sochi, RussiaPlant Biology and Biotechnology Department, Sirius University of Science and Technology, 1 Olympic Avenue, 354340 Sochi, RussiaDepartment of Genetics and Biotechnology, Saint Petersburg State University, 7/9 Universitetskaya Emb, 199034 St. Petersburg, RussiaDepartment of Genetics and Biotechnology, Saint Petersburg State University, 7/9 Universitetskaya Emb, 199034 St. Petersburg, RussiaDepartment of Genetics and Biotechnology, Saint Petersburg State University, 7/9 Universitetskaya Emb, 199034 St. Petersburg, RussiaWOX family transcription factors are well-known regulators of plant development, controlling cell proliferation and differentiation in diverse organs and tissues. Several <i>WOX</i> genes have been shown to participate in regeneration processes which take place in plant cell cultures in vitro, but the effects of most of them on tissue culture development have not been discovered yet. In this study, we evaluated the effects of <i>MtWOX2</i> gene overexpression on the embryogenic callus development and transcriptomic state in <i>Medicago truncatula</i>. According to our results, overexpression of <i>MtWOX2</i> leads to an increase in callus weight. Furthermore, transcriptomic changes in <i>MtWOX2</i> overexpressing calli are, to a large extent, opposite to the changes caused by overexpression of <i>MtWOX9-1</i>, a somatic embryogenesis stimulator. These results add new information about the mechanisms of interaction between different <i>WOX</i> genes and can be useful for the search of new regeneration regulators.https://www.mdpi.com/2223-7747/13/1/102transcription factorsWOXcallussomatic embryogenesis |
| spellingShingle | Elizaveta Y. Krasnoperova Varvara E. Tvorogova Kirill V. Smirnov Elena P. Efremova Elina A. Potsenkovskaia Anastasia M. Artemiuk Zakhar S. Konstantinov Veronika Y. Simonova Anna V. Brynchikova Daria V. Yakovleva Daria B. Pavlova Ludmila A. Lutova <i>MtWOX2</i> and <i>MtWOX9-1</i> Effects on the Embryogenic Callus Transcriptome in <i>Medicago truncatula</i> Plants transcription factors WOX callus somatic embryogenesis |
| title | <i>MtWOX2</i> and <i>MtWOX9-1</i> Effects on the Embryogenic Callus Transcriptome in <i>Medicago truncatula</i> |
| title_full | <i>MtWOX2</i> and <i>MtWOX9-1</i> Effects on the Embryogenic Callus Transcriptome in <i>Medicago truncatula</i> |
| title_fullStr | <i>MtWOX2</i> and <i>MtWOX9-1</i> Effects on the Embryogenic Callus Transcriptome in <i>Medicago truncatula</i> |
| title_full_unstemmed | <i>MtWOX2</i> and <i>MtWOX9-1</i> Effects on the Embryogenic Callus Transcriptome in <i>Medicago truncatula</i> |
| title_short | <i>MtWOX2</i> and <i>MtWOX9-1</i> Effects on the Embryogenic Callus Transcriptome in <i>Medicago truncatula</i> |
| title_sort | i mtwox2 i and i mtwox9 1 i effects on the embryogenic callus transcriptome in i medicago truncatula i |
| topic | transcription factors WOX callus somatic embryogenesis |
| url | https://www.mdpi.com/2223-7747/13/1/102 |
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