AFM studies of DNA structures extracted from adriamycin treated and non-treated Erlich tumor cells
Atomic force microscopy (AFM), a unique tool to investigate drug treatment of cancer cells, was used to analyze the anti-neoplastic activity of adriamycin by comparing DNA structures of non-treated and adriamycin-treated Ehrlich tumor cells. The non-treated cells exhibited a highly branched intact c...
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2005-01-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2005/0352-51390506823A.pdf |
| _version_ | 1818983914395402240 |
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| author | Avramov-Ivić M. Petrović Slobodan D. Kalman E. Milosavljević T. Reljin I. Reljin B. Bogdanović G. Baltić Vit.V. Keresztes Z. |
| author_facet | Avramov-Ivić M. Petrović Slobodan D. Kalman E. Milosavljević T. Reljin I. Reljin B. Bogdanović G. Baltić Vit.V. Keresztes Z. |
| author_sort | Avramov-Ivić M. |
| collection | DOAJ |
| description | Atomic force microscopy (AFM), a unique tool to investigate drug treatment of cancer cells, was used to analyze the anti-neoplastic activity of adriamycin by comparing DNA structures of non-treated and adriamycin-treated Ehrlich tumor cells. The non-treated cells exhibited a highly branched intact chromatin structure, related to the intensive DNA replication in cancer cells. Images from adriamycin-treated tumor cells showed that the DNA chains were broken and the chromatin structure had been destroyed. Possible explanations for these effects of adriamycin are considered: breakage of hydrogen bonding, oxidation and intercalation effects, as well as the poisoning of topoisomerase enzyme. DNA fractal and multifractal analyses performed in order to evaluate the degree of bond scission, showed that the treated DNA had become more fractal compared to non-treated DNA. |
| first_indexed | 2024-12-20T18:10:41Z |
| format | Article |
| id | doaj.art-27a640ba01b94391b354488f5b98e556 |
| institution | Directory Open Access Journal |
| issn | 0352-5139 1820-7421 |
| language | English |
| last_indexed | 2024-12-20T18:10:41Z |
| publishDate | 2005-01-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-27a640ba01b94391b354488f5b98e5562022-12-21T19:30:28ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51391820-74212005-01-0170682383110.2298/JSC0506823A0352-51390506823AAFM studies of DNA structures extracted from adriamycin treated and non-treated Erlich tumor cellsAvramov-Ivić M.0Petrović Slobodan D.1Kalman E.2Milosavljević T.3Reljin I.4Reljin B.5Bogdanović G.6Baltić Vit.V.7Keresztes Z.8ICTM, Institute of Electrochemistry, University of Belgrade, BelgradeFaculty of Technology and Metallurgy - Department of Organic Chemistry, Belgrade and Hemofarm Group, Pharmaceutical-Chemical Industry, VršacChemical Research Center, Department of Nanostructures and Surface Modification, Budapest, HungaryHemofarm Group, Pharmaceutical-Chemical Industry, VršacFaculty of Electrical Engineering, BelgradeFaculty of Electrical Engineering, BelgradeInstitute of Oncology, Sremska KamenicaInstitute of Oncology, Sremska KamenicaChemical Research Center, Department of Nanostructures and Surface Modification, Budapest, HungaryAtomic force microscopy (AFM), a unique tool to investigate drug treatment of cancer cells, was used to analyze the anti-neoplastic activity of adriamycin by comparing DNA structures of non-treated and adriamycin-treated Ehrlich tumor cells. The non-treated cells exhibited a highly branched intact chromatin structure, related to the intensive DNA replication in cancer cells. Images from adriamycin-treated tumor cells showed that the DNA chains were broken and the chromatin structure had been destroyed. Possible explanations for these effects of adriamycin are considered: breakage of hydrogen bonding, oxidation and intercalation effects, as well as the poisoning of topoisomerase enzyme. DNA fractal and multifractal analyses performed in order to evaluate the degree of bond scission, showed that the treated DNA had become more fractal compared to non-treated DNA.http://www.doiserbia.nb.rs/img/doi/0352-5139/2005/0352-51390506823A.pdfadriamycinehrlich tumor cellsatomic force microscopytopoisomerasefractal and multifractal analysis |
| spellingShingle | Avramov-Ivić M. Petrović Slobodan D. Kalman E. Milosavljević T. Reljin I. Reljin B. Bogdanović G. Baltić Vit.V. Keresztes Z. AFM studies of DNA structures extracted from adriamycin treated and non-treated Erlich tumor cells Journal of the Serbian Chemical Society adriamycin ehrlich tumor cells atomic force microscopy topoisomerase fractal and multifractal analysis |
| title | AFM studies of DNA structures extracted from adriamycin treated and non-treated Erlich tumor cells |
| title_full | AFM studies of DNA structures extracted from adriamycin treated and non-treated Erlich tumor cells |
| title_fullStr | AFM studies of DNA structures extracted from adriamycin treated and non-treated Erlich tumor cells |
| title_full_unstemmed | AFM studies of DNA structures extracted from adriamycin treated and non-treated Erlich tumor cells |
| title_short | AFM studies of DNA structures extracted from adriamycin treated and non-treated Erlich tumor cells |
| title_sort | afm studies of dna structures extracted from adriamycin treated and non treated erlich tumor cells |
| topic | adriamycin ehrlich tumor cells atomic force microscopy topoisomerase fractal and multifractal analysis |
| url | http://www.doiserbia.nb.rs/img/doi/0352-5139/2005/0352-51390506823A.pdf |
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