Changes in Oral Microbial Diversity in a Piglet Model of Traumatic Brain Injury
Dynamic changes in the oral microbiome have gained attention due to their potential diagnostic role in neurological diseases such as Alzheimer’s disease and Parkinson’s disease. Traumatic brain injury (TBI) is a leading cause of death and disability in the United States, but no studies have examined...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Brain Sciences |
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| Online Access: | https://www.mdpi.com/2076-3425/12/8/1111 |
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| author | Julie Heejin Jeon Jeferson M. Lourenco Madison M. Fagan Christina B. Welch Sydney E. Sneed Stephanie Dubrof Kylee J. Duberstein Todd R. Callaway Franklin D. West Hea Jin Park |
| author_facet | Julie Heejin Jeon Jeferson M. Lourenco Madison M. Fagan Christina B. Welch Sydney E. Sneed Stephanie Dubrof Kylee J. Duberstein Todd R. Callaway Franklin D. West Hea Jin Park |
| author_sort | Julie Heejin Jeon |
| collection | DOAJ |
| description | Dynamic changes in the oral microbiome have gained attention due to their potential diagnostic role in neurological diseases such as Alzheimer’s disease and Parkinson’s disease. Traumatic brain injury (TBI) is a leading cause of death and disability in the United States, but no studies have examined the changes in oral microbiome during the acute stage of TBI using a clinically translational pig model. Crossbred piglets (4–5 weeks old, male) underwent either a controlled cortical impact (TBI, <i>n</i> = 6) or sham surgery (sham, <i>n</i> = 6). The oral microbiome parameters were quantified from the upper and lower gingiva, both buccal mucosa, and floor of the mouth pre-surgery and 1, 3, and 7 days post-surgery (PS) using the 16S rRNA gene. Faith’s phylogenetic diversity was significantly lower in the TBI piglets at 7 days PS compared to those of sham, and beta diversity at 1, 3, and 7 days PS was significantly different between TBI and sham piglets. However, no significant changes in the taxonomic composition of the oral microbiome were observed following TBI compared to sham. Further studies are needed to investigate the potential diagnostic role of the oral microbiome during the chronic stage of TBI with a larger number of subjects. |
| first_indexed | 2024-03-09T04:38:48Z |
| format | Article |
| id | doaj.art-27a6e5dab53748289ebd1a7a6b7330c1 |
| institution | Directory Open Access Journal |
| issn | 2076-3425 |
| language | English |
| last_indexed | 2024-03-09T04:38:48Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Brain Sciences |
| spelling | doaj.art-27a6e5dab53748289ebd1a7a6b7330c12023-12-03T13:23:49ZengMDPI AGBrain Sciences2076-34252022-08-01128111110.3390/brainsci12081111Changes in Oral Microbial Diversity in a Piglet Model of Traumatic Brain InjuryJulie Heejin Jeon0Jeferson M. Lourenco1Madison M. Fagan2Christina B. Welch3Sydney E. Sneed4Stephanie Dubrof5Kylee J. Duberstein6Todd R. Callaway7Franklin D. West8Hea Jin Park9Department of Nutritional Sciences, College of Family and Consumer Sciences, University of Georgia, Athens, GA 30602, USADepartment of Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia, Athens, GA 30602, USADepartment of Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia, Athens, GA 30602, USADepartment of Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia, Athens, GA 30602, USADepartment of Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia, Athens, GA 30602, USADepartment of Nutritional Sciences, College of Family and Consumer Sciences, University of Georgia, Athens, GA 30602, USADepartment of Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia, Athens, GA 30602, USADepartment of Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia, Athens, GA 30602, USADepartment of Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia, Athens, GA 30602, USADepartment of Nutritional Sciences, College of Family and Consumer Sciences, University of Georgia, Athens, GA 30602, USADynamic changes in the oral microbiome have gained attention due to their potential diagnostic role in neurological diseases such as Alzheimer’s disease and Parkinson’s disease. Traumatic brain injury (TBI) is a leading cause of death and disability in the United States, but no studies have examined the changes in oral microbiome during the acute stage of TBI using a clinically translational pig model. Crossbred piglets (4–5 weeks old, male) underwent either a controlled cortical impact (TBI, <i>n</i> = 6) or sham surgery (sham, <i>n</i> = 6). The oral microbiome parameters were quantified from the upper and lower gingiva, both buccal mucosa, and floor of the mouth pre-surgery and 1, 3, and 7 days post-surgery (PS) using the 16S rRNA gene. Faith’s phylogenetic diversity was significantly lower in the TBI piglets at 7 days PS compared to those of sham, and beta diversity at 1, 3, and 7 days PS was significantly different between TBI and sham piglets. However, no significant changes in the taxonomic composition of the oral microbiome were observed following TBI compared to sham. Further studies are needed to investigate the potential diagnostic role of the oral microbiome during the chronic stage of TBI with a larger number of subjects.https://www.mdpi.com/2076-3425/12/8/1111oral microbiomeneurological diseaseporcine model |
| spellingShingle | Julie Heejin Jeon Jeferson M. Lourenco Madison M. Fagan Christina B. Welch Sydney E. Sneed Stephanie Dubrof Kylee J. Duberstein Todd R. Callaway Franklin D. West Hea Jin Park Changes in Oral Microbial Diversity in a Piglet Model of Traumatic Brain Injury Brain Sciences oral microbiome neurological disease porcine model |
| title | Changes in Oral Microbial Diversity in a Piglet Model of Traumatic Brain Injury |
| title_full | Changes in Oral Microbial Diversity in a Piglet Model of Traumatic Brain Injury |
| title_fullStr | Changes in Oral Microbial Diversity in a Piglet Model of Traumatic Brain Injury |
| title_full_unstemmed | Changes in Oral Microbial Diversity in a Piglet Model of Traumatic Brain Injury |
| title_short | Changes in Oral Microbial Diversity in a Piglet Model of Traumatic Brain Injury |
| title_sort | changes in oral microbial diversity in a piglet model of traumatic brain injury |
| topic | oral microbiome neurological disease porcine model |
| url | https://www.mdpi.com/2076-3425/12/8/1111 |
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