Microtubule Assists Actomyosin to Regulate Cell Nuclear Mechanics and Chromatin Accessibility
Cellular behaviors and functions can be regulated by mechanical cues from microenvironments, which are transmitted to nucleus through the physical connections of cytoskeletons in the cells. How these physical connections determine transcriptional activity were not clearly known. The actomyosin, whic...
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| Format: | Article |
| Language: | English |
| Published: |
American Association for the Advancement of Science (AAAS)
2023-01-01
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| Series: | Research |
| Online Access: | https://spj.science.org/doi/10.34133/research.0054 |
| _version_ | 1827296604285042688 |
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| author | Jiwen Geng Zhefeng Kang Qian Sun Man Zhang Peng Wang Yupei Li Jiameng Li Baihai Su Qiang Wei |
| author_facet | Jiwen Geng Zhefeng Kang Qian Sun Man Zhang Peng Wang Yupei Li Jiameng Li Baihai Su Qiang Wei |
| author_sort | Jiwen Geng |
| collection | DOAJ |
| description | Cellular behaviors and functions can be regulated by mechanical cues from microenvironments, which are transmitted to nucleus through the physical connections of cytoskeletons in the cells. How these physical connections determine transcriptional activity were not clearly known. The actomyosin, which generates intracellular traction force, has been recognized to control the nuclear morphology. Here, we have revealed that microtubule, the stiffest cytoskeleton, is also involved in the process of nuclear morphology alteration. The microtubule negatively regulates the actomyosin-induced nuclear invaginations but not the nuclear wrinkles. Moreover, these nuclear shape changes are proven to mediate the chromatin remodeling, which essentially mediates cell gene expression and phenotype determination. The actomyosin disruption leads to the loss of chromatin accessibility, which can be partly recovered by microtubule interference through nuclear shape control. This finding answers the question of how mechanical cues regulate chromatin accessibility and cell behaviors. It also provides new insights into cell mechanotransduction and nuclear mechanics. |
| first_indexed | 2024-03-13T07:12:40Z |
| format | Article |
| id | doaj.art-27a725c0c61e4a2cab1fe1717294151f |
| institution | Directory Open Access Journal |
| issn | 2639-5274 |
| language | English |
| last_indexed | 2024-04-24T14:41:25Z |
| publishDate | 2023-01-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | Research |
| spelling | doaj.art-27a725c0c61e4a2cab1fe1717294151f2024-04-02T21:01:31ZengAmerican Association for the Advancement of Science (AAAS)Research2639-52742023-01-01610.34133/research.0054Microtubule Assists Actomyosin to Regulate Cell Nuclear Mechanics and Chromatin AccessibilityJiwen Geng0Zhefeng Kang1Qian Sun2Man Zhang3Peng Wang4Yupei Li5Jiameng Li6Baihai Su7Qiang Wei8Department of Nephrology, West China Hospital, Sichuan University, Chengdu, 610041, China.Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, 610041, China.College of Polymer Science and Engineering, College of Biomedical Engineering, State Key Laboratory of Polymer Materials and Engineering Sichuan University, Chengdu, 610065, China.College of Polymer Science and Engineering, College of Biomedical Engineering, State Key Laboratory of Polymer Materials and Engineering Sichuan University, Chengdu, 610065, China.College of Polymer Science and Engineering, College of Biomedical Engineering, State Key Laboratory of Polymer Materials and Engineering Sichuan University, Chengdu, 610065, China.Department of Nephrology, West China Hospital, Sichuan University, Chengdu, 610041, China.Department of Nephrology, West China Hospital, Sichuan University, Chengdu, 610041, China.Department of Nephrology, West China Hospital, Sichuan University, Chengdu, 610041, China.College of Polymer Science and Engineering, College of Biomedical Engineering, State Key Laboratory of Polymer Materials and Engineering Sichuan University, Chengdu, 610065, China.Cellular behaviors and functions can be regulated by mechanical cues from microenvironments, which are transmitted to nucleus through the physical connections of cytoskeletons in the cells. How these physical connections determine transcriptional activity were not clearly known. The actomyosin, which generates intracellular traction force, has been recognized to control the nuclear morphology. Here, we have revealed that microtubule, the stiffest cytoskeleton, is also involved in the process of nuclear morphology alteration. The microtubule negatively regulates the actomyosin-induced nuclear invaginations but not the nuclear wrinkles. Moreover, these nuclear shape changes are proven to mediate the chromatin remodeling, which essentially mediates cell gene expression and phenotype determination. The actomyosin disruption leads to the loss of chromatin accessibility, which can be partly recovered by microtubule interference through nuclear shape control. This finding answers the question of how mechanical cues regulate chromatin accessibility and cell behaviors. It also provides new insights into cell mechanotransduction and nuclear mechanics.https://spj.science.org/doi/10.34133/research.0054 |
| spellingShingle | Jiwen Geng Zhefeng Kang Qian Sun Man Zhang Peng Wang Yupei Li Jiameng Li Baihai Su Qiang Wei Microtubule Assists Actomyosin to Regulate Cell Nuclear Mechanics and Chromatin Accessibility Research |
| title | Microtubule Assists Actomyosin to Regulate Cell Nuclear Mechanics and Chromatin Accessibility |
| title_full | Microtubule Assists Actomyosin to Regulate Cell Nuclear Mechanics and Chromatin Accessibility |
| title_fullStr | Microtubule Assists Actomyosin to Regulate Cell Nuclear Mechanics and Chromatin Accessibility |
| title_full_unstemmed | Microtubule Assists Actomyosin to Regulate Cell Nuclear Mechanics and Chromatin Accessibility |
| title_short | Microtubule Assists Actomyosin to Regulate Cell Nuclear Mechanics and Chromatin Accessibility |
| title_sort | microtubule assists actomyosin to regulate cell nuclear mechanics and chromatin accessibility |
| url | https://spj.science.org/doi/10.34133/research.0054 |
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