<i>Pistacia lentiscus</i> L. Distilled Leaves as a Potential Cosmeceutical Ingredient: Phytochemical Characterization, Transdermal Diffusion, and Anti-Elastase and Anti-Tyrosinase Activities
The present work was performed to investigate the phenolic composition of <i>P. lentiscus</i> L. distilled leaves (PDL) and examine its potential against certain key enzymes related to skin aging. High-pressure liquid chromatography coupled to mass spectrometry (HPLC-MS) and various sepa...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/3/855 |
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| author | Wiem Elloumi Amina Maalej Sergio Ortiz Sylvie Michel Mohamed Chamkha Sabrina Boutefnouchet Sami Sayadi |
| author_facet | Wiem Elloumi Amina Maalej Sergio Ortiz Sylvie Michel Mohamed Chamkha Sabrina Boutefnouchet Sami Sayadi |
| author_sort | Wiem Elloumi |
| collection | DOAJ |
| description | The present work was performed to investigate the phenolic composition of <i>P. lentiscus</i> L. distilled leaves (PDL) and examine its potential against certain key enzymes related to skin aging. High-pressure liquid chromatography coupled to mass spectrometry (HPLC-MS) and various separation procedures combined with nuclear magnetic resonance (NMR) and MS analysis were performed to isolate and identify compounds present in the ethyl acetate extract (EAE) of PDL. A high amount of flavonol glycoside was detected in EAE. Indeed, quercetin-3-<i>O</i>-rhamnoside (FC), myricetin-3-<i>O</i>-rhamnoside (FM2), and kaempferol-3-<i>O</i>-rhamnoside (FB2) were isolated from EAE, and are present in high quantities of 10.47 ± 0.26, 12.17 ± 0.74, and 4.53 ± 0.59 mg/g dry weight, respectively. A transdermal diffusion study was carried out to determine the EAE-molecules that may transmit the cutaneous barrier and showed that FM2 transmits the membrane barrier with a high amount followed by FC. EAE, FM2, and FC were tested against tyrosinase and elastase enzymes. Moreover, intracellular tyrosinase inhibition and cytotoxicity on skin melanoma cells (B16) were evaluated. The results indicated that EAE, FC, and FM2 have important inhibitory activities compared to the well-known standards, at non-cytotoxic concentrations. Therefore, they could be excellent agents for treating skin pigmentation and elasticity problems. |
| first_indexed | 2024-03-09T23:28:01Z |
| format | Article |
| id | doaj.art-27a75059970f4e448e1fa338c09b7fd8 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T23:28:01Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-27a75059970f4e448e1fa338c09b7fd82023-11-23T17:13:30ZengMDPI AGMolecules1420-30492022-01-0127385510.3390/molecules27030855<i>Pistacia lentiscus</i> L. Distilled Leaves as a Potential Cosmeceutical Ingredient: Phytochemical Characterization, Transdermal Diffusion, and Anti-Elastase and Anti-Tyrosinase ActivitiesWiem Elloumi0Amina Maalej1Sergio Ortiz2Sylvie Michel3Mohamed Chamkha4Sabrina Boutefnouchet5Sami Sayadi6Laboratory of Environmental Bioprocesses, Center of Biotechnology of Sfax, University of Sfax, Sfax 3018, TunisiaLaboratory of Environmental Bioprocesses, Center of Biotechnology of Sfax, University of Sfax, Sfax 3018, TunisiaCiTCoM, CNRS 8038, PNAS Team (Natural Products, Analysis and Synthesis), Faculté de Santé, Université de Paris, 4, av. de l’Observatoire, 75006 Paris, FranceCiTCoM, CNRS 8038, PNAS Team (Natural Products, Analysis and Synthesis), Faculté de Santé, Université de Paris, 4, av. de l’Observatoire, 75006 Paris, FranceLaboratory of Environmental Bioprocesses, Center of Biotechnology of Sfax, University of Sfax, Sfax 3018, TunisiaCiTCoM, CNRS 8038, PNAS Team (Natural Products, Analysis and Synthesis), Faculté de Santé, Université de Paris, 4, av. de l’Observatoire, 75006 Paris, FranceBiotechnology Program, Center for Sustainable Development, College of Arts and Sciences, Qatar University, Doha 2713, QatarThe present work was performed to investigate the phenolic composition of <i>P. lentiscus</i> L. distilled leaves (PDL) and examine its potential against certain key enzymes related to skin aging. High-pressure liquid chromatography coupled to mass spectrometry (HPLC-MS) and various separation procedures combined with nuclear magnetic resonance (NMR) and MS analysis were performed to isolate and identify compounds present in the ethyl acetate extract (EAE) of PDL. A high amount of flavonol glycoside was detected in EAE. Indeed, quercetin-3-<i>O</i>-rhamnoside (FC), myricetin-3-<i>O</i>-rhamnoside (FM2), and kaempferol-3-<i>O</i>-rhamnoside (FB2) were isolated from EAE, and are present in high quantities of 10.47 ± 0.26, 12.17 ± 0.74, and 4.53 ± 0.59 mg/g dry weight, respectively. A transdermal diffusion study was carried out to determine the EAE-molecules that may transmit the cutaneous barrier and showed that FM2 transmits the membrane barrier with a high amount followed by FC. EAE, FM2, and FC were tested against tyrosinase and elastase enzymes. Moreover, intracellular tyrosinase inhibition and cytotoxicity on skin melanoma cells (B16) were evaluated. The results indicated that EAE, FC, and FM2 have important inhibitory activities compared to the well-known standards, at non-cytotoxic concentrations. Therefore, they could be excellent agents for treating skin pigmentation and elasticity problems.https://www.mdpi.com/1420-3049/27/3/855<i>Pistacia lentiscus</i> L. leavesLC-MSnuclear magnetic resonancequercetin-3-<i>O</i>-rhamnosidemyricetin-3-<i>O</i>-rhamnosidetransdermal diffusion |
| spellingShingle | Wiem Elloumi Amina Maalej Sergio Ortiz Sylvie Michel Mohamed Chamkha Sabrina Boutefnouchet Sami Sayadi <i>Pistacia lentiscus</i> L. Distilled Leaves as a Potential Cosmeceutical Ingredient: Phytochemical Characterization, Transdermal Diffusion, and Anti-Elastase and Anti-Tyrosinase Activities Molecules <i>Pistacia lentiscus</i> L. leaves LC-MS nuclear magnetic resonance quercetin-3-<i>O</i>-rhamnoside myricetin-3-<i>O</i>-rhamnoside transdermal diffusion |
| title | <i>Pistacia lentiscus</i> L. Distilled Leaves as a Potential Cosmeceutical Ingredient: Phytochemical Characterization, Transdermal Diffusion, and Anti-Elastase and Anti-Tyrosinase Activities |
| title_full | <i>Pistacia lentiscus</i> L. Distilled Leaves as a Potential Cosmeceutical Ingredient: Phytochemical Characterization, Transdermal Diffusion, and Anti-Elastase and Anti-Tyrosinase Activities |
| title_fullStr | <i>Pistacia lentiscus</i> L. Distilled Leaves as a Potential Cosmeceutical Ingredient: Phytochemical Characterization, Transdermal Diffusion, and Anti-Elastase and Anti-Tyrosinase Activities |
| title_full_unstemmed | <i>Pistacia lentiscus</i> L. Distilled Leaves as a Potential Cosmeceutical Ingredient: Phytochemical Characterization, Transdermal Diffusion, and Anti-Elastase and Anti-Tyrosinase Activities |
| title_short | <i>Pistacia lentiscus</i> L. Distilled Leaves as a Potential Cosmeceutical Ingredient: Phytochemical Characterization, Transdermal Diffusion, and Anti-Elastase and Anti-Tyrosinase Activities |
| title_sort | i pistacia lentiscus i l distilled leaves as a potential cosmeceutical ingredient phytochemical characterization transdermal diffusion and anti elastase and anti tyrosinase activities |
| topic | <i>Pistacia lentiscus</i> L. leaves LC-MS nuclear magnetic resonance quercetin-3-<i>O</i>-rhamnoside myricetin-3-<i>O</i>-rhamnoside transdermal diffusion |
| url | https://www.mdpi.com/1420-3049/27/3/855 |
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