A sampling strategy for genome sequencing the British terrestrial arthropod fauna [version 1; peer review: 2 approved]

The Darwin Tree of Life (DToL) project aims to sequence and assemble high-quality genomes from all eukaryote species in Britain and Ireland, with the first phase of the project concentrating on family-level coverage plus species of particular ecological, biomedical or evolutionary interest. We summa...

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Bibliographic Details
Main Authors: Craig R. Macadam, Ian Barnes, Heather Allen, Christopher Fletcher, Gavin R. Broad, Inez Januszczak, Peter W.H. Holland, Peter O. Mulhair, Owen T. Lewis, Benjamin W. Price, Lyndall Pereira da Conceicoa, Laura Sivess, Chris Raper, Liam Crowley, Olga Sivell, Mara Lawniczak, Douglas Boyes
Format: Article
Language:English
Published: Wellcome 2023-03-01
Series:Wellcome Open Research
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Online Access:https://wellcomeopenresearch.org/articles/8-123/v1
Description
Summary:The Darwin Tree of Life (DToL) project aims to sequence and assemble high-quality genomes from all eukaryote species in Britain and Ireland, with the first phase of the project concentrating on family-level coverage plus species of particular ecological, biomedical or evolutionary interest. We summarise the processes involved in (1) assessing the UK arthropod fauna and the status of individual species on UK lists; (2) prioritising and collecting species for initial genome sequencing; (3) handling methods to ensure that high-quality genomic DNA is preserved; and (4) compiling standard operating procedures for processing specimens for genome sequencing, identification verification and voucher specimen curation. We briefly explore some lessons learned from the pilot phase of DToL and the impact of the Covid-19 pandemic.
ISSN:2398-502X