A sampling strategy for genome sequencing the British terrestrial arthropod fauna [version 1; peer review: 2 approved]
The Darwin Tree of Life (DToL) project aims to sequence and assemble high-quality genomes from all eukaryote species in Britain and Ireland, with the first phase of the project concentrating on family-level coverage plus species of particular ecological, biomedical or evolutionary interest. We summa...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wellcome
2023-03-01
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| Series: | Wellcome Open Research |
| Subjects: | |
| Online Access: | https://wellcomeopenresearch.org/articles/8-123/v1 |
| _version_ | 1797788509911646208 |
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| author | Craig R. Macadam Ian Barnes Heather Allen Christopher Fletcher Gavin R. Broad Inez Januszczak Peter W.H. Holland Peter O. Mulhair Owen T. Lewis Benjamin W. Price Lyndall Pereira da Conceicoa Laura Sivess Chris Raper Liam Crowley Olga Sivell Mara Lawniczak Douglas Boyes |
| author_facet | Craig R. Macadam Ian Barnes Heather Allen Christopher Fletcher Gavin R. Broad Inez Januszczak Peter W.H. Holland Peter O. Mulhair Owen T. Lewis Benjamin W. Price Lyndall Pereira da Conceicoa Laura Sivess Chris Raper Liam Crowley Olga Sivell Mara Lawniczak Douglas Boyes |
| author_sort | Craig R. Macadam |
| collection | DOAJ |
| description | The Darwin Tree of Life (DToL) project aims to sequence and assemble high-quality genomes from all eukaryote species in Britain and Ireland, with the first phase of the project concentrating on family-level coverage plus species of particular ecological, biomedical or evolutionary interest. We summarise the processes involved in (1) assessing the UK arthropod fauna and the status of individual species on UK lists; (2) prioritising and collecting species for initial genome sequencing; (3) handling methods to ensure that high-quality genomic DNA is preserved; and (4) compiling standard operating procedures for processing specimens for genome sequencing, identification verification and voucher specimen curation. We briefly explore some lessons learned from the pilot phase of DToL and the impact of the Covid-19 pandemic. |
| first_indexed | 2024-03-13T01:37:35Z |
| format | Article |
| id | doaj.art-27a7f79657b041298e4b13270b7ad342 |
| institution | Directory Open Access Journal |
| issn | 2398-502X |
| language | English |
| last_indexed | 2024-03-13T01:37:35Z |
| publishDate | 2023-03-01 |
| publisher | Wellcome |
| record_format | Article |
| series | Wellcome Open Research |
| spelling | doaj.art-27a7f79657b041298e4b13270b7ad3422023-07-04T01:00:02ZengWellcomeWellcome Open Research2398-502X2023-03-01820983A sampling strategy for genome sequencing the British terrestrial arthropod fauna [version 1; peer review: 2 approved]Craig R. Macadam0Ian Barnes1https://orcid.org/0000-0001-8322-6918Heather Allen2Christopher Fletcher3https://orcid.org/0000-0002-1944-5048Gavin R. Broad4https://orcid.org/0000-0001-7223-5333Inez Januszczak5Peter W.H. Holland6Peter O. Mulhair7https://orcid.org/0000-0003-3311-4883Owen T. Lewis8https://orcid.org/0000-0001-7935-6111Benjamin W. Price9https://orcid.org/0000-0001-5497-4087Lyndall Pereira da Conceicoa10Laura Sivess11Chris Raper12Liam Crowley13https://orcid.org/0000-0001-6380-0329Olga Sivell14https://orcid.org/0000-0001-7394-8546Mara Lawniczak15https://orcid.org/0000-0002-3006-2080Douglas Boyes16Natural History Museum, London, SW7 5BD, UKNatural History Museum, London, SW7 5BD, UKNatural History Museum, London, SW7 5BD, UKNatural History Museum, London, SW7 5BD, UKNatural History Museum, London, SW7 5BD, UKNatural History Museum, London, SW7 5BD, UKBiology, University of Oxford, Oxford, Oxfordshire, OX1 3SZ, UKBiology, University of Oxford, Oxford, Oxfordshire, OX1 3SZ, UKBiology, University of Oxford, Oxford, Oxfordshire, OX1 3SZ, UKNatural History Museum, London, SW7 5BD, UKNatural History Museum, London, SW7 5BD, UKNatural History Museum, London, SW7 5BD, UKNatural History Museum, London, SW7 5BD, UKBiology, University of Oxford, Oxford, Oxfordshire, OX1 3SZ, UKNatural History Museum, London, SW7 5BD, UKTree of Life, Wellcome Trust Sanger Institute, Hinxton, CB10 1SA, UKBiology, University of Oxford, Oxford, Oxfordshire, OX1 3SZ, UKThe Darwin Tree of Life (DToL) project aims to sequence and assemble high-quality genomes from all eukaryote species in Britain and Ireland, with the first phase of the project concentrating on family-level coverage plus species of particular ecological, biomedical or evolutionary interest. We summarise the processes involved in (1) assessing the UK arthropod fauna and the status of individual species on UK lists; (2) prioritising and collecting species for initial genome sequencing; (3) handling methods to ensure that high-quality genomic DNA is preserved; and (4) compiling standard operating procedures for processing specimens for genome sequencing, identification verification and voucher specimen curation. We briefly explore some lessons learned from the pilot phase of DToL and the impact of the Covid-19 pandemic.https://wellcomeopenresearch.org/articles/8-123/v1Earth BioGenome Project Invertebrate Whole-Genome Sequencing Collecting Preservation Prioritisationeng |
| spellingShingle | Craig R. Macadam Ian Barnes Heather Allen Christopher Fletcher Gavin R. Broad Inez Januszczak Peter W.H. Holland Peter O. Mulhair Owen T. Lewis Benjamin W. Price Lyndall Pereira da Conceicoa Laura Sivess Chris Raper Liam Crowley Olga Sivell Mara Lawniczak Douglas Boyes A sampling strategy for genome sequencing the British terrestrial arthropod fauna [version 1; peer review: 2 approved] Wellcome Open Research Earth BioGenome Project Invertebrate Whole-Genome Sequencing Collecting Preservation Prioritisation eng |
| title | A sampling strategy for genome sequencing the British terrestrial arthropod fauna [version 1; peer review: 2 approved] |
| title_full | A sampling strategy for genome sequencing the British terrestrial arthropod fauna [version 1; peer review: 2 approved] |
| title_fullStr | A sampling strategy for genome sequencing the British terrestrial arthropod fauna [version 1; peer review: 2 approved] |
| title_full_unstemmed | A sampling strategy for genome sequencing the British terrestrial arthropod fauna [version 1; peer review: 2 approved] |
| title_short | A sampling strategy for genome sequencing the British terrestrial arthropod fauna [version 1; peer review: 2 approved] |
| title_sort | sampling strategy for genome sequencing the british terrestrial arthropod fauna version 1 peer review 2 approved |
| topic | Earth BioGenome Project Invertebrate Whole-Genome Sequencing Collecting Preservation Prioritisation eng |
| url | https://wellcomeopenresearch.org/articles/8-123/v1 |
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