Candida albicans and Candida dubliniensis Show Different Trailing Effect Patterns When Exposed to Echinocandins and Azoles
When Candida albicans and Candida dubliniensis isolates were tested for susceptibility to fluconazole and echinocandins using either EUCAST or Etest methods, differential patterns of growth were observed, independently of the methods used. For C. albicans, a trailing phenomenon (incomplete growth in...
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| Format: | Article |
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.01286/full |
| _version_ | 1818291427324461056 |
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| author | Rania Ayadi Emilie Sitterlé Emilie Sitterlé Christophe d’Enfert Eric Dannaoui Eric Dannaoui Marie-Elisabeth Bougnoux Marie-Elisabeth Bougnoux |
| author_facet | Rania Ayadi Emilie Sitterlé Emilie Sitterlé Christophe d’Enfert Eric Dannaoui Eric Dannaoui Marie-Elisabeth Bougnoux Marie-Elisabeth Bougnoux |
| author_sort | Rania Ayadi |
| collection | DOAJ |
| description | When Candida albicans and Candida dubliniensis isolates were tested for susceptibility to fluconazole and echinocandins using either EUCAST or Etest methods, differential patterns of growth were observed, independently of the methods used. For C. albicans, a trailing phenomenon (incomplete growth inhibition at supra-MICs) was observed with fluconazole in 90% and 93.3% for EUCAST and Etest, respectively, but not with echinocandins (<7% for EUCAST and 0% for Etest). In contrast, for C. dubliniensis, a trailing phenomenon was very rarely observed with fluconazole (20% for EUCAST and 0% for Etest), while the opposite pattern was observed with echinocandins (>50% for EUCAST and >86% for Etest). This suggests that the pathways involved in the trailing effect might be different between these two related species. Furthermore, clinical microbiologists must be aware of these species-specific patterns for a reliable MIC determination. |
| first_indexed | 2024-12-13T02:43:53Z |
| format | Article |
| id | doaj.art-27a878b6219c4c678fcddd82d969b086 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T02:43:53Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-27a878b6219c4c678fcddd82d969b0862022-12-22T00:02:14ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-06-011110.3389/fmicb.2020.01286521728Candida albicans and Candida dubliniensis Show Different Trailing Effect Patterns When Exposed to Echinocandins and AzolesRania Ayadi0Emilie Sitterlé1Emilie Sitterlé2Christophe d’Enfert3Eric Dannaoui4Eric Dannaoui5Marie-Elisabeth Bougnoux6Marie-Elisabeth Bougnoux7Unité de Parasitologie-Mycologie, Service de Microbiologie, Faculté de Médecine, APHP, Hôpital Européen Georges Pompidou, Université Paris-Descartes, Paris, FranceUnité de Parasitologie-Mycologie, Service de Microbiologie, Faculté de Médecine, APHP, Hôpital Necker Enfants-Malades, Université Paris-Descartes, Paris, FranceUnité Biologie et Pathogénicité Fongiques, Institut Pasteur, USC 2019 INRA, Paris, FranceUnité Biologie et Pathogénicité Fongiques, Institut Pasteur, USC 2019 INRA, Paris, FranceUnité de Parasitologie-Mycologie, Service de Microbiologie, Faculté de Médecine, APHP, Hôpital Européen Georges Pompidou, Université Paris-Descartes, Paris, FranceDynamyc Research Group, Paris Est Créteil University (UPEC, EnvA), Créteil, FranceUnité de Parasitologie-Mycologie, Service de Microbiologie, Faculté de Médecine, APHP, Hôpital Necker Enfants-Malades, Université Paris-Descartes, Paris, FranceUnité Biologie et Pathogénicité Fongiques, Institut Pasteur, USC 2019 INRA, Paris, FranceWhen Candida albicans and Candida dubliniensis isolates were tested for susceptibility to fluconazole and echinocandins using either EUCAST or Etest methods, differential patterns of growth were observed, independently of the methods used. For C. albicans, a trailing phenomenon (incomplete growth inhibition at supra-MICs) was observed with fluconazole in 90% and 93.3% for EUCAST and Etest, respectively, but not with echinocandins (<7% for EUCAST and 0% for Etest). In contrast, for C. dubliniensis, a trailing phenomenon was very rarely observed with fluconazole (20% for EUCAST and 0% for Etest), while the opposite pattern was observed with echinocandins (>50% for EUCAST and >86% for Etest). This suggests that the pathways involved in the trailing effect might be different between these two related species. Furthermore, clinical microbiologists must be aware of these species-specific patterns for a reliable MIC determination.https://www.frontiersin.org/article/10.3389/fmicb.2020.01286/fullC. albicansC. dubliniensistrailingEUCASTantifungal susceptibility testingEtest |
| spellingShingle | Rania Ayadi Emilie Sitterlé Emilie Sitterlé Christophe d’Enfert Eric Dannaoui Eric Dannaoui Marie-Elisabeth Bougnoux Marie-Elisabeth Bougnoux Candida albicans and Candida dubliniensis Show Different Trailing Effect Patterns When Exposed to Echinocandins and Azoles Frontiers in Microbiology C. albicans C. dubliniensis trailing EUCAST antifungal susceptibility testing Etest |
| title | Candida albicans and Candida dubliniensis Show Different Trailing Effect Patterns When Exposed to Echinocandins and Azoles |
| title_full | Candida albicans and Candida dubliniensis Show Different Trailing Effect Patterns When Exposed to Echinocandins and Azoles |
| title_fullStr | Candida albicans and Candida dubliniensis Show Different Trailing Effect Patterns When Exposed to Echinocandins and Azoles |
| title_full_unstemmed | Candida albicans and Candida dubliniensis Show Different Trailing Effect Patterns When Exposed to Echinocandins and Azoles |
| title_short | Candida albicans and Candida dubliniensis Show Different Trailing Effect Patterns When Exposed to Echinocandins and Azoles |
| title_sort | candida albicans and candida dubliniensis show different trailing effect patterns when exposed to echinocandins and azoles |
| topic | C. albicans C. dubliniensis trailing EUCAST antifungal susceptibility testing Etest |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.01286/full |
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