FENDRR Sponges miR-424-5p to Inhibit Cell Proliferation, Migration and Invasion in Colorectal Cancer
Objective: LncRNAs are non-coding RNAs exerting vital roles in the occurrence and development of various cancer types. This study tended to describe the expression pattern of FENDRR in colorectal cancer (CRC), and further investigate the role of FENDRR in CRC cell biological behaviors. Methods: Gene...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2020-12-01
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| Series: | Technology in Cancer Research & Treatment |
| Online Access: | https://doi.org/10.1177/1533033820980102 |
| _version_ | 1818381726022369280 |
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| author | Chuan Cheng MM Huixia Li BS Jiujian Zheng BS Jie Xu BS Peng Gao BS Jianping Wang BS |
| author_facet | Chuan Cheng MM Huixia Li BS Jiujian Zheng BS Jie Xu BS Peng Gao BS Jianping Wang BS |
| author_sort | Chuan Cheng MM |
| collection | DOAJ |
| description | Objective: LncRNAs are non-coding RNAs exerting vital roles in the occurrence and development of various cancer types. This study tended to describe the expression pattern of FENDRR in colorectal cancer (CRC), and further investigate the role of FENDRR in CRC cell biological behaviors. Methods: Gene expression profile of colon cancer was accessed from the TCGA database, and then processed for differential analysis for identification of differentially expressed lncRNAs and miRNAs. Some in vitro experiments like qRT-PCR, MTT, colony formation assay, wound healing assay and Transwell assay were performed to assess the effect of FENDRR on cell biological behaviors. Dual-luciferase reporter assay was conducted to further validate the targeting relationship between FENDRR and miR-424-5p, and rescue experiments were carried out for determining the mechanism of FENDRR/miR-424-5p underlying the proliferation, migration and invasion of CRC cells. Results: Bioinformatics analysis suggested that FENDRR was significantly down-regulated in CRC tissue, and low FENDRR was intimately correlated to poor prognosis. FENDRR overexpression could greatly inhibit cell proliferation, migration and invasion. Besides, there was a negative correlation between FENDRR and miR-424-5p. Dual-luciferase reporter assay indicated that miR-424-5p was a direct target of FENDRR. Rescue experiments discovered that FENDRR exerted its role in cell proliferation, migration and invasion in CRC via targeting miR-424-5p. Conclusion: FENDRR is poorly expressed in CRC tissue and cells, and low FENDRR is responsible for the inhibition of cell proliferation, migration and invasion of CRC by means of targeting miR-424-5p. |
| first_indexed | 2024-12-14T02:39:09Z |
| format | Article |
| id | doaj.art-27ac5fa6b9b048f8a54176406db64f7b |
| institution | Directory Open Access Journal |
| issn | 1533-0338 |
| language | English |
| last_indexed | 2024-12-14T02:39:09Z |
| publishDate | 2020-12-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Technology in Cancer Research & Treatment |
| spelling | doaj.art-27ac5fa6b9b048f8a54176406db64f7b2022-12-21T23:20:03ZengSAGE PublishingTechnology in Cancer Research & Treatment1533-03382020-12-011910.1177/1533033820980102FENDRR Sponges miR-424-5p to Inhibit Cell Proliferation, Migration and Invasion in Colorectal CancerChuan Cheng MM0Huixia Li BS1Jiujian Zheng BS2Jie Xu BS3Peng Gao BS4Jianping Wang BS5 Department of Colorectal and Anal Surgery, Lishui Municipal Central Hospital, Zhejiang Province, China Department of Colorectal and Anal Surgery, Lishui Municipal Central Hospital, Zhejiang Province, China Department of Colorectal and Anal Surgery, Lishui Municipal Central Hospital, Zhejiang Province, China Department of Colorectal and Anal Surgery, Lishui Municipal Central Hospital, Zhejiang Province, China Department of Colorectal and Anal Surgery, Lishui Municipal Central Hospital, Zhejiang Province, China Department of Colorectal and Anal Surgery, Lishui Municipal Central Hospital, Zhejiang Province, ChinaObjective: LncRNAs are non-coding RNAs exerting vital roles in the occurrence and development of various cancer types. This study tended to describe the expression pattern of FENDRR in colorectal cancer (CRC), and further investigate the role of FENDRR in CRC cell biological behaviors. Methods: Gene expression profile of colon cancer was accessed from the TCGA database, and then processed for differential analysis for identification of differentially expressed lncRNAs and miRNAs. Some in vitro experiments like qRT-PCR, MTT, colony formation assay, wound healing assay and Transwell assay were performed to assess the effect of FENDRR on cell biological behaviors. Dual-luciferase reporter assay was conducted to further validate the targeting relationship between FENDRR and miR-424-5p, and rescue experiments were carried out for determining the mechanism of FENDRR/miR-424-5p underlying the proliferation, migration and invasion of CRC cells. Results: Bioinformatics analysis suggested that FENDRR was significantly down-regulated in CRC tissue, and low FENDRR was intimately correlated to poor prognosis. FENDRR overexpression could greatly inhibit cell proliferation, migration and invasion. Besides, there was a negative correlation between FENDRR and miR-424-5p. Dual-luciferase reporter assay indicated that miR-424-5p was a direct target of FENDRR. Rescue experiments discovered that FENDRR exerted its role in cell proliferation, migration and invasion in CRC via targeting miR-424-5p. Conclusion: FENDRR is poorly expressed in CRC tissue and cells, and low FENDRR is responsible for the inhibition of cell proliferation, migration and invasion of CRC by means of targeting miR-424-5p.https://doi.org/10.1177/1533033820980102 |
| spellingShingle | Chuan Cheng MM Huixia Li BS Jiujian Zheng BS Jie Xu BS Peng Gao BS Jianping Wang BS FENDRR Sponges miR-424-5p to Inhibit Cell Proliferation, Migration and Invasion in Colorectal Cancer Technology in Cancer Research & Treatment |
| title | FENDRR Sponges miR-424-5p to Inhibit Cell Proliferation, Migration and Invasion in Colorectal Cancer |
| title_full | FENDRR Sponges miR-424-5p to Inhibit Cell Proliferation, Migration and Invasion in Colorectal Cancer |
| title_fullStr | FENDRR Sponges miR-424-5p to Inhibit Cell Proliferation, Migration and Invasion in Colorectal Cancer |
| title_full_unstemmed | FENDRR Sponges miR-424-5p to Inhibit Cell Proliferation, Migration and Invasion in Colorectal Cancer |
| title_short | FENDRR Sponges miR-424-5p to Inhibit Cell Proliferation, Migration and Invasion in Colorectal Cancer |
| title_sort | fendrr sponges mir 424 5p to inhibit cell proliferation migration and invasion in colorectal cancer |
| url | https://doi.org/10.1177/1533033820980102 |
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