A new multiplex assay of 17 autosomal STRs and Amelogenin for forensic application.
This paper describes a newly devised autosomal short tandem repeat (STR) multiplex polymerase chain reaction (PCR) systems for 17 autosomal loci (D1S1656, D2S441, D3S1358, D3S3045, D6S477, D7S3048, D8S1132, D10S1435, D10S1248, D11S2368, D13S325, D14S608, D15S659, D17S1290, D18S535, D19S253 and D22-G...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3581461?pdf=render |
| _version_ | 1819163442639011840 |
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| author | Suhua Zhang Huaizhou Tian Jun Wu Shumin Zhao Chengtao Li |
| author_facet | Suhua Zhang Huaizhou Tian Jun Wu Shumin Zhao Chengtao Li |
| author_sort | Suhua Zhang |
| collection | DOAJ |
| description | This paper describes a newly devised autosomal short tandem repeat (STR) multiplex polymerase chain reaction (PCR) systems for 17 autosomal loci (D1S1656, D2S441, D3S1358, D3S3045, D6S477, D7S3048, D8S1132, D10S1435, D10S1248, D11S2368, D13S325, D14S608, D15S659, D17S1290, D18S535, D19S253 and D22-GATA198B05) and Amelogenin. Primers for the loci were designed and optimized so that all of the amplicons were distributed from 50 base pairs (bp) to less than 500 bp within a five-dye chemistry design with the fifth dye reserved for the sizing standard. Strategies were developed to overcome challenges that encountered in creating the final assay. The limits of the multiplex were tested, resulting in the successful amplification of genomic DNA range from 0.25-4 ng with 30 PCR cycles. A total of 681 individuals from the Chinese Han population were studied and forensic genetic data were present. No significant deviations from Hardy-Weinberg equilibrium were observed. A total of 180 alleles were detected for the 17 autosomal STRs. The cumulative mean exclusion chance in duos (CMECD) was 0.999967, and cumulative mean exclusion chance in trios (CMECT) was 0.99999995. We conclude that the present 17plex autosomal STRs assay provides an additional powerful tool for forensic applications. |
| first_indexed | 2024-12-22T17:44:12Z |
| format | Article |
| id | doaj.art-27ad0b3805904e95acc5a58881bb7c5c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T17:44:12Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-27ad0b3805904e95acc5a58881bb7c5c2022-12-21T18:18:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0182e5747110.1371/journal.pone.0057471A new multiplex assay of 17 autosomal STRs and Amelogenin for forensic application.Suhua ZhangHuaizhou TianJun WuShumin ZhaoChengtao LiThis paper describes a newly devised autosomal short tandem repeat (STR) multiplex polymerase chain reaction (PCR) systems for 17 autosomal loci (D1S1656, D2S441, D3S1358, D3S3045, D6S477, D7S3048, D8S1132, D10S1435, D10S1248, D11S2368, D13S325, D14S608, D15S659, D17S1290, D18S535, D19S253 and D22-GATA198B05) and Amelogenin. Primers for the loci were designed and optimized so that all of the amplicons were distributed from 50 base pairs (bp) to less than 500 bp within a five-dye chemistry design with the fifth dye reserved for the sizing standard. Strategies were developed to overcome challenges that encountered in creating the final assay. The limits of the multiplex were tested, resulting in the successful amplification of genomic DNA range from 0.25-4 ng with 30 PCR cycles. A total of 681 individuals from the Chinese Han population were studied and forensic genetic data were present. No significant deviations from Hardy-Weinberg equilibrium were observed. A total of 180 alleles were detected for the 17 autosomal STRs. The cumulative mean exclusion chance in duos (CMECD) was 0.999967, and cumulative mean exclusion chance in trios (CMECT) was 0.99999995. We conclude that the present 17plex autosomal STRs assay provides an additional powerful tool for forensic applications.http://europepmc.org/articles/PMC3581461?pdf=render |
| spellingShingle | Suhua Zhang Huaizhou Tian Jun Wu Shumin Zhao Chengtao Li A new multiplex assay of 17 autosomal STRs and Amelogenin for forensic application. PLoS ONE |
| title | A new multiplex assay of 17 autosomal STRs and Amelogenin for forensic application. |
| title_full | A new multiplex assay of 17 autosomal STRs and Amelogenin for forensic application. |
| title_fullStr | A new multiplex assay of 17 autosomal STRs and Amelogenin for forensic application. |
| title_full_unstemmed | A new multiplex assay of 17 autosomal STRs and Amelogenin for forensic application. |
| title_short | A new multiplex assay of 17 autosomal STRs and Amelogenin for forensic application. |
| title_sort | new multiplex assay of 17 autosomal strs and amelogenin for forensic application |
| url | http://europepmc.org/articles/PMC3581461?pdf=render |
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