Autologous Adipose-Derived Mesenchymal Stem Cells Combined with Shockwave Therapy Synergistically Ameliorates the Osteoarthritic Pathological Factors in Knee Joint
Adipose-derived mesenchymal stem cells (ADSCs) and shockwave (SW) therapy have been shown to exert a chondroprotective effect for osteoarthritis (OA). The results of this study demonstrated that autologous ADSCs had dose-dependent and synergistic effects with SW therapy (0.25 mJ/mm<sup>2</s...
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MDPI AG
2021-04-01
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| Online Access: | https://www.mdpi.com/1424-8247/14/4/318 |
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| author | Jai-Hong Cheng Ke-Tien Yen Wen-Yi Chou Shun-Wun Jhan Shan-Ling Hsu Jih-Yang Ko Ching-Jen Wang Chun-En Aurea Kuo Szu-Ying Wu Tsai-Chin Hsu Chieh-Cheng Hsu |
| author_facet | Jai-Hong Cheng Ke-Tien Yen Wen-Yi Chou Shun-Wun Jhan Shan-Ling Hsu Jih-Yang Ko Ching-Jen Wang Chun-En Aurea Kuo Szu-Ying Wu Tsai-Chin Hsu Chieh-Cheng Hsu |
| author_sort | Jai-Hong Cheng |
| collection | DOAJ |
| description | Adipose-derived mesenchymal stem cells (ADSCs) and shockwave (SW) therapy have been shown to exert a chondroprotective effect for osteoarthritis (OA). The results of this study demonstrated that autologous ADSCs had dose-dependent and synergistic effects with SW therapy (0.25 mJ/mm<sup>2</sup> with 800 impulses) in OA rat knee joint. Autologous, high-dose 2 × 10<sup>6</sup> ADSCs (ADSC2 group) combined with SW therapy significantly increased the bone volume, trabecular thickness, and trabecular number among in the treatment groups. ADSC2 combined with SW therapy significantly reduced the synovitis score and OARSI score in comparison with other treatments. In the analysis of inflammation-induced extracellular matrix factors of the articular cartilage in OA, the results displayed that ADSC2 combined with SW therapy had a greater than other treatments in terms of reducing tumor necrosis factor-inducible gene (TSG)-6 and proteoglycan (PRG)-4, in addition to increasing tissue inhibitor matrix metalloproteinase (TIMP)-1 and type II collagen. Furthermore, ADSC2 combined with SW therapy significantly reduced the expression of inflammation-induced bone morphogenetic protein (BMP)-2 and BMP-6. Therefore, the results demonstrated that ADSC2 combined with SW therapy had a synergistic effect to ameliorate osteoarthritic pathological factors in OA joints. |
| first_indexed | 2024-03-10T12:41:19Z |
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| id | doaj.art-27b2934900df4ba28635611669d14208 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T12:41:19Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-27b2934900df4ba28635611669d142082023-11-21T13:50:27ZengMDPI AGPharmaceuticals1424-82472021-04-0114431810.3390/ph14040318Autologous Adipose-Derived Mesenchymal Stem Cells Combined with Shockwave Therapy Synergistically Ameliorates the Osteoarthritic Pathological Factors in Knee JointJai-Hong Cheng0Ke-Tien Yen1Wen-Yi Chou2Shun-Wun Jhan3Shan-Ling Hsu4Jih-Yang Ko5Ching-Jen Wang6Chun-En Aurea Kuo7Szu-Ying Wu8Tsai-Chin Hsu9Chieh-Cheng Hsu10Center for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, TaiwanDepartment of Leisure and Sports Management, Cheng Shiu University, Kaohsiung 833, TaiwanCenter for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, TaiwanCenter for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, TaiwanCenter for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, TaiwanCenter for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, TaiwanCenter for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, TaiwanDepartment of Chinese Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833401, TaiwanDepartment of Chinese Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833401, TaiwanCenter for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, TaiwanCenter for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, TaiwanAdipose-derived mesenchymal stem cells (ADSCs) and shockwave (SW) therapy have been shown to exert a chondroprotective effect for osteoarthritis (OA). The results of this study demonstrated that autologous ADSCs had dose-dependent and synergistic effects with SW therapy (0.25 mJ/mm<sup>2</sup> with 800 impulses) in OA rat knee joint. Autologous, high-dose 2 × 10<sup>6</sup> ADSCs (ADSC2 group) combined with SW therapy significantly increased the bone volume, trabecular thickness, and trabecular number among in the treatment groups. ADSC2 combined with SW therapy significantly reduced the synovitis score and OARSI score in comparison with other treatments. In the analysis of inflammation-induced extracellular matrix factors of the articular cartilage in OA, the results displayed that ADSC2 combined with SW therapy had a greater than other treatments in terms of reducing tumor necrosis factor-inducible gene (TSG)-6 and proteoglycan (PRG)-4, in addition to increasing tissue inhibitor matrix metalloproteinase (TIMP)-1 and type II collagen. Furthermore, ADSC2 combined with SW therapy significantly reduced the expression of inflammation-induced bone morphogenetic protein (BMP)-2 and BMP-6. Therefore, the results demonstrated that ADSC2 combined with SW therapy had a synergistic effect to ameliorate osteoarthritic pathological factors in OA joints.https://www.mdpi.com/1424-8247/14/4/318adipose-derived mesenchymal stem cellsshockwaveosteoarthritisextracellular matrix factorsbone morphogenetic protein |
| spellingShingle | Jai-Hong Cheng Ke-Tien Yen Wen-Yi Chou Shun-Wun Jhan Shan-Ling Hsu Jih-Yang Ko Ching-Jen Wang Chun-En Aurea Kuo Szu-Ying Wu Tsai-Chin Hsu Chieh-Cheng Hsu Autologous Adipose-Derived Mesenchymal Stem Cells Combined with Shockwave Therapy Synergistically Ameliorates the Osteoarthritic Pathological Factors in Knee Joint Pharmaceuticals adipose-derived mesenchymal stem cells shockwave osteoarthritis extracellular matrix factors bone morphogenetic protein |
| title | Autologous Adipose-Derived Mesenchymal Stem Cells Combined with Shockwave Therapy Synergistically Ameliorates the Osteoarthritic Pathological Factors in Knee Joint |
| title_full | Autologous Adipose-Derived Mesenchymal Stem Cells Combined with Shockwave Therapy Synergistically Ameliorates the Osteoarthritic Pathological Factors in Knee Joint |
| title_fullStr | Autologous Adipose-Derived Mesenchymal Stem Cells Combined with Shockwave Therapy Synergistically Ameliorates the Osteoarthritic Pathological Factors in Knee Joint |
| title_full_unstemmed | Autologous Adipose-Derived Mesenchymal Stem Cells Combined with Shockwave Therapy Synergistically Ameliorates the Osteoarthritic Pathological Factors in Knee Joint |
| title_short | Autologous Adipose-Derived Mesenchymal Stem Cells Combined with Shockwave Therapy Synergistically Ameliorates the Osteoarthritic Pathological Factors in Knee Joint |
| title_sort | autologous adipose derived mesenchymal stem cells combined with shockwave therapy synergistically ameliorates the osteoarthritic pathological factors in knee joint |
| topic | adipose-derived mesenchymal stem cells shockwave osteoarthritis extracellular matrix factors bone morphogenetic protein |
| url | https://www.mdpi.com/1424-8247/14/4/318 |
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