The Milk Microbiota of the Spanish Churra Sheep Breed: New Insights into the Complexity of the Milk Microbiome of Dairy Species
Milk from healthy animals has classically been considered a sterile fluid. With the development of massively parallel sequencing and its application to the study of the microbiome of different body fluids, milk microbiota has been documented in several animal species. In this study, the main objecti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/9/1463 |
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| author | Cristina Esteban-Blanco Beatriz Gutiérrez-Gil Héctor Marina Rocío Pelayo Aroa Suárez-Vega Alberto Acedo Juan-José Arranz |
| author_facet | Cristina Esteban-Blanco Beatriz Gutiérrez-Gil Héctor Marina Rocío Pelayo Aroa Suárez-Vega Alberto Acedo Juan-José Arranz |
| author_sort | Cristina Esteban-Blanco |
| collection | DOAJ |
| description | Milk from healthy animals has classically been considered a sterile fluid. With the development of massively parallel sequencing and its application to the study of the microbiome of different body fluids, milk microbiota has been documented in several animal species. In this study, the main objective of this work was to access bacterial profiles of healthy milk samples using the next-generation sequencing of amplicons from the 16S rRNA gene to characterise the milk microbiome of the Churra breed. A total of 212 samples were collected from two Churra dairy farms with a different management system. The core milk microbiota in Churra ewes includes lesser genera (only two taxa: Staphylococcus and Escherichia/Shigella) than studies reported in other dairy species or even in a previous study in Assaf sheep milk. We found that diversity values in the two flocks of Churra breed were lower than the diversity of the milk microbiota in Assaf. The non-metric multidimensional scaling (NMDS) ordination using Bray-Curtis distance separates samples based on their microbiota composition. The information reported here might be used to understand the complex issue of milk microbiota composition. |
| first_indexed | 2024-03-10T17:06:38Z |
| format | Article |
| id | doaj.art-27b9253576554209ba7e93afe59b9af6 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T17:06:38Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-27b9253576554209ba7e93afe59b9af62023-11-20T10:47:37ZengMDPI AGAnimals2076-26152020-08-01109146310.3390/ani10091463The Milk Microbiota of the Spanish Churra Sheep Breed: New Insights into the Complexity of the Milk Microbiome of Dairy SpeciesCristina Esteban-Blanco0Beatriz Gutiérrez-Gil1Héctor Marina2Rocío Pelayo3Aroa Suárez-Vega4Alberto Acedo5Juan-José Arranz6Departamento de Producción Animal, Facultad de Veterinaria, Universidad de León, Campus de Vegazana s/n, 24071 León, SpainDepartamento de Producción Animal, Facultad de Veterinaria, Universidad de León, Campus de Vegazana s/n, 24071 León, SpainDepartamento de Producción Animal, Facultad de Veterinaria, Universidad de León, Campus de Vegazana s/n, 24071 León, SpainDepartamento de Producción Animal, Facultad de Veterinaria, Universidad de León, Campus de Vegazana s/n, 24071 León, SpainDepartamento de Producción Animal, Facultad de Veterinaria, Universidad de León, Campus de Vegazana s/n, 24071 León, SpainBiome Makers Inc., West Sacramento, CA 95605, USADepartamento de Producción Animal, Facultad de Veterinaria, Universidad de León, Campus de Vegazana s/n, 24071 León, SpainMilk from healthy animals has classically been considered a sterile fluid. With the development of massively parallel sequencing and its application to the study of the microbiome of different body fluids, milk microbiota has been documented in several animal species. In this study, the main objective of this work was to access bacterial profiles of healthy milk samples using the next-generation sequencing of amplicons from the 16S rRNA gene to characterise the milk microbiome of the Churra breed. A total of 212 samples were collected from two Churra dairy farms with a different management system. The core milk microbiota in Churra ewes includes lesser genera (only two taxa: Staphylococcus and Escherichia/Shigella) than studies reported in other dairy species or even in a previous study in Assaf sheep milk. We found that diversity values in the two flocks of Churra breed were lower than the diversity of the milk microbiota in Assaf. The non-metric multidimensional scaling (NMDS) ordination using Bray-Curtis distance separates samples based on their microbiota composition. The information reported here might be used to understand the complex issue of milk microbiota composition.https://www.mdpi.com/2076-2615/10/9/1463AssafChurradairy sheepmilk microbiota16S rRNA gene sequencing |
| spellingShingle | Cristina Esteban-Blanco Beatriz Gutiérrez-Gil Héctor Marina Rocío Pelayo Aroa Suárez-Vega Alberto Acedo Juan-José Arranz The Milk Microbiota of the Spanish Churra Sheep Breed: New Insights into the Complexity of the Milk Microbiome of Dairy Species Animals Assaf Churra dairy sheep milk microbiota 16S rRNA gene sequencing |
| title | The Milk Microbiota of the Spanish Churra Sheep Breed: New Insights into the Complexity of the Milk Microbiome of Dairy Species |
| title_full | The Milk Microbiota of the Spanish Churra Sheep Breed: New Insights into the Complexity of the Milk Microbiome of Dairy Species |
| title_fullStr | The Milk Microbiota of the Spanish Churra Sheep Breed: New Insights into the Complexity of the Milk Microbiome of Dairy Species |
| title_full_unstemmed | The Milk Microbiota of the Spanish Churra Sheep Breed: New Insights into the Complexity of the Milk Microbiome of Dairy Species |
| title_short | The Milk Microbiota of the Spanish Churra Sheep Breed: New Insights into the Complexity of the Milk Microbiome of Dairy Species |
| title_sort | milk microbiota of the spanish churra sheep breed new insights into the complexity of the milk microbiome of dairy species |
| topic | Assaf Churra dairy sheep milk microbiota 16S rRNA gene sequencing |
| url | https://www.mdpi.com/2076-2615/10/9/1463 |
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