DNA Damage of Lung Cells from Immature Cadmium-Ingested Mice
The objective of this study was to investigate the effects of cadmium on DNA damage of lung cells in immature animals. Seventy-two immature mice were randomly divided into twelve cadmium-ingested groups including low dose (1/100 LD50, 1.87 mg/kg BW), middle dose (1/50 LD50, 3.74 mg/kg BW), high dose...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Agriculture, Faisalabad
2014-01-01
|
| Series: | Pakistan Veterinary Journal |
| Subjects: | |
| Online Access: | http://pvj.com.pk/pdf-files/34_1/73-77.pdf |
| _version_ | 1819039060240367616 |
|---|---|
| author | Xue-feng Yang Hai-tang Zhang Guo-ying Fan Dong-yang Liu Ya-ming Ge Jin-qing Jiang Zi-liang Wang |
| author_facet | Xue-feng Yang Hai-tang Zhang Guo-ying Fan Dong-yang Liu Ya-ming Ge Jin-qing Jiang Zi-liang Wang |
| author_sort | Xue-feng Yang |
| collection | DOAJ |
| description | The objective of this study was to investigate the effects of cadmium on DNA damage of lung cells in immature animals. Seventy-two immature mice were randomly divided into twelve cadmium-ingested groups including low dose (1/100 LD50, 1.87 mg/kg BW), middle dose (1/50 LD50, 3.74 mg/kg BW), high dose (1/25 LD50, 7.48 mg/kg BW) and control group, and exposed to cadmium chloride for 10, 20 and 30 days, respectively. Mice were sacrificed after cadmium exposure for different time, and lung cells were collected to investigate DNA damage by comet assay. The results showed that comet tailing ratio, tail length, comet length, tail moment, Olive tail moment and damaged grade of lung cells from immature mice increased along with increasing of cadmium exposure dose and time. In low dose group treated for 30 days, there was significance (P<0.05) in comet length or high significance (P<0.01) in other parameters compared with control group or low dose group treated for 10 days. When mice were exposed to cadmium at high dose for 30 days, DNA of lung cells was damaged most seriously. Our results indicate that cadmium can induce DNA damage of lung cells from immature mice in dose- dependent and time-dependent manners, and DNA will be damaged when immature mice exposed to cadmium for long time even at low dose. Meanwhile, comet assay can be considered as a powerful and sensitive biomarker assay in risk assessment of immature animals exposed to cadmium. |
| first_indexed | 2024-12-21T08:47:12Z |
| format | Article |
| id | doaj.art-27b9d8c8f63c41898c1141e621181f52 |
| institution | Directory Open Access Journal |
| issn | 0253-8318 2074-7764 |
| language | English |
| last_indexed | 2024-12-21T08:47:12Z |
| publishDate | 2014-01-01 |
| publisher | University of Agriculture, Faisalabad |
| record_format | Article |
| series | Pakistan Veterinary Journal |
| spelling | doaj.art-27b9d8c8f63c41898c1141e621181f522022-12-21T19:09:47ZengUniversity of Agriculture, FaisalabadPakistan Veterinary Journal0253-83182074-77642014-01-013417377DNA Damage of Lung Cells from Immature Cadmium-Ingested MiceXue-feng Yang0Hai-tang Zhang1Guo-ying Fan2Dong-yang Liu3Ya-ming Ge4Jin-qing Jiang5Zi-liang Wang6College of Animal Science, Henan Institute of Science and Technology, Xinxiang, Henan Province, P.R. China, 453003College of Animal Science, Henan Institute of Science and Technology, Xinxiang, Henan Province, P.R. China, 453003College of Animal Science, Henan Institute of Science and Technology, Xinxiang, Henan Province, P.R. China, 453003College of Animal Science, Henan Institute of Science and Technology, Xinxiang, Henan Province, P.R. China, 453003College of Animal Science, Henan Institute of Science and Technology, Xinxiang, Henan Province, P.R. China, 453003College of Animal Science, Henan Institute of Science and Technology, Xinxiang, Henan Province, P.R. China, 453003College of Animal Science, Henan Institute of Science and Technology, Xinxiang, Henan Province, P.R. China, 453003The objective of this study was to investigate the effects of cadmium on DNA damage of lung cells in immature animals. Seventy-two immature mice were randomly divided into twelve cadmium-ingested groups including low dose (1/100 LD50, 1.87 mg/kg BW), middle dose (1/50 LD50, 3.74 mg/kg BW), high dose (1/25 LD50, 7.48 mg/kg BW) and control group, and exposed to cadmium chloride for 10, 20 and 30 days, respectively. Mice were sacrificed after cadmium exposure for different time, and lung cells were collected to investigate DNA damage by comet assay. The results showed that comet tailing ratio, tail length, comet length, tail moment, Olive tail moment and damaged grade of lung cells from immature mice increased along with increasing of cadmium exposure dose and time. In low dose group treated for 30 days, there was significance (P<0.05) in comet length or high significance (P<0.01) in other parameters compared with control group or low dose group treated for 10 days. When mice were exposed to cadmium at high dose for 30 days, DNA of lung cells was damaged most seriously. Our results indicate that cadmium can induce DNA damage of lung cells from immature mice in dose- dependent and time-dependent manners, and DNA will be damaged when immature mice exposed to cadmium for long time even at low dose. Meanwhile, comet assay can be considered as a powerful and sensitive biomarker assay in risk assessment of immature animals exposed to cadmium.http://pvj.com.pk/pdf-files/34_1/73-77.pdfCadmiumComet assayDNA damageImmature miceLung cell |
| spellingShingle | Xue-feng Yang Hai-tang Zhang Guo-ying Fan Dong-yang Liu Ya-ming Ge Jin-qing Jiang Zi-liang Wang DNA Damage of Lung Cells from Immature Cadmium-Ingested Mice Pakistan Veterinary Journal Cadmium Comet assay DNA damage Immature mice Lung cell |
| title | DNA Damage of Lung Cells from Immature Cadmium-Ingested Mice |
| title_full | DNA Damage of Lung Cells from Immature Cadmium-Ingested Mice |
| title_fullStr | DNA Damage of Lung Cells from Immature Cadmium-Ingested Mice |
| title_full_unstemmed | DNA Damage of Lung Cells from Immature Cadmium-Ingested Mice |
| title_short | DNA Damage of Lung Cells from Immature Cadmium-Ingested Mice |
| title_sort | dna damage of lung cells from immature cadmium ingested mice |
| topic | Cadmium Comet assay DNA damage Immature mice Lung cell |
| url | http://pvj.com.pk/pdf-files/34_1/73-77.pdf |
| work_keys_str_mv | AT xuefengyang dnadamageoflungcellsfromimmaturecadmiumingestedmice AT haitangzhang dnadamageoflungcellsfromimmaturecadmiumingestedmice AT guoyingfan dnadamageoflungcellsfromimmaturecadmiumingestedmice AT dongyangliu dnadamageoflungcellsfromimmaturecadmiumingestedmice AT yamingge dnadamageoflungcellsfromimmaturecadmiumingestedmice AT jinqingjiang dnadamageoflungcellsfromimmaturecadmiumingestedmice AT ziliangwang dnadamageoflungcellsfromimmaturecadmiumingestedmice |