High frequency somatic embryogenesis and plant regeneration of interspecific ginseng hybrid between Panax ginseng and Panax quinquefolius
Background: Interspecific ginseng hybrid, Panax ginseng × Panax quenquifolius (Pgq) has vigorous growth and produces larger roots than its parents. However, F1 progenies are complete male sterile. Plant tissue culture technology can circumvent the issue and propagate the hybrid. Methods: Murashige a...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-01-01
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| Series: | Journal of Ginseng Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1226845316302366 |
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| author | Jong Youn Kim Prakash Babu Adhikari Chang Ho Ahn Dong Hwi Kim Young Chang Kim Jung Yeon Han Subramanyam Kondeti Yong Eui Choi |
| author_facet | Jong Youn Kim Prakash Babu Adhikari Chang Ho Ahn Dong Hwi Kim Young Chang Kim Jung Yeon Han Subramanyam Kondeti Yong Eui Choi |
| author_sort | Jong Youn Kim |
| collection | DOAJ |
| description | Background: Interspecific ginseng hybrid, Panax ginseng × Panax quenquifolius (Pgq) has vigorous growth and produces larger roots than its parents. However, F1 progenies are complete male sterile. Plant tissue culture technology can circumvent the issue and propagate the hybrid. Methods: Murashige and Skoog (MS) medium with different concentrations (0, 2, 4, and 6 mg/L) of 2,4-dichlorophenoxyacetic acid (2,4-D) was used for callus induction and somatic embryogenesis (SE). The embryos, after culturing on GA3 supplemented medium, were transferred to hormone free ½ Schenk and Hildebrandt (SH) medium. The developed taproots with dormant buds were treated with GA3 to break the bud dormancy, and transferred to soil. Hybrid Pgq plants were verified by random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses and by LC-IT-TOF-MS. Results: We conducted a comparative study of somatic embryogenesis (SE) in Pgq and its parents, and attempted to establish the soil transfer of in vitro propagated Pgq tap roots. The Pgq explants showed higher rate of embryogenesis (~56% at 2 mg/L 2,4-D concentration) as well as higher number of embryos per explants (~7 at the same 2,4-D concentration) compared to its either parents. The germinated embryos, after culturing on GA3 supplemented medium, were transferred to hormone free ½ SH medium to support the continued growth and kept until nutrient depletion induced senescence (NuDIS) of leaf defoliation occurred (4 months). By that time, thickened tap roots with well-developed lateral roots and dormant buds were obtained. All Pgq tap roots pretreated with 20 mg/L GA3 for at least a week produced new shoots after soil transfer. We selected the discriminatory RAPD and ISSR markers to find the interspecific ginseng hybrid among its parents. The F1 hybrid (Pgq) contained species specific 2 ginsenosides (ginsenoside Rf in P. ginseng and pseudoginsenosides F11 in P. quinquefolius), and higher amount of other ginsenosides than its parents. Conclusion: Micropropagation of interspecific hybrid ginseng can give an opportunity for continuous production of plants. Keywords: hybrid, micropropagation, Panax ginseng, Panax quinquefolius, somatic embryogenesis |
| first_indexed | 2024-04-12T01:29:19Z |
| format | Article |
| id | doaj.art-27baff4b16a641848f5f8b8fb0c9cebd |
| institution | Directory Open Access Journal |
| issn | 1226-8453 |
| language | English |
| last_indexed | 2024-04-12T01:29:19Z |
| publishDate | 2019-01-01 |
| publisher | Elsevier |
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| series | Journal of Ginseng Research |
| spelling | doaj.art-27baff4b16a641848f5f8b8fb0c9cebd2022-12-22T03:53:32ZengElsevierJournal of Ginseng Research1226-84532019-01-014313848High frequency somatic embryogenesis and plant regeneration of interspecific ginseng hybrid between Panax ginseng and Panax quinquefoliusJong Youn Kim0Prakash Babu Adhikari1Chang Ho Ahn2Dong Hwi Kim3Young Chang Kim4Jung Yeon Han5Subramanyam Kondeti6Yong Eui Choi7Department of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon, Republic of KoreaDepartment of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon, Republic of KoreaDepartment of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon, Republic of KoreaDepartment of Herbal Crop Research, National Institute of Horticulture and Herbal Science, Rural Development Administration, Eumseong, Republic of KoreaDepartment of Herbal Crop Research, National Institute of Horticulture and Herbal Science, Rural Development Administration, Eumseong, Republic of KoreaDepartment of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon, Republic of KoreaDepartment of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon, Republic of KoreaDepartment of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon, Republic of Korea; Corresponding author. Department of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon-si, Gangwon-do, 24341, Republic of Korea.Background: Interspecific ginseng hybrid, Panax ginseng × Panax quenquifolius (Pgq) has vigorous growth and produces larger roots than its parents. However, F1 progenies are complete male sterile. Plant tissue culture technology can circumvent the issue and propagate the hybrid. Methods: Murashige and Skoog (MS) medium with different concentrations (0, 2, 4, and 6 mg/L) of 2,4-dichlorophenoxyacetic acid (2,4-D) was used for callus induction and somatic embryogenesis (SE). The embryos, after culturing on GA3 supplemented medium, were transferred to hormone free ½ Schenk and Hildebrandt (SH) medium. The developed taproots with dormant buds were treated with GA3 to break the bud dormancy, and transferred to soil. Hybrid Pgq plants were verified by random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses and by LC-IT-TOF-MS. Results: We conducted a comparative study of somatic embryogenesis (SE) in Pgq and its parents, and attempted to establish the soil transfer of in vitro propagated Pgq tap roots. The Pgq explants showed higher rate of embryogenesis (~56% at 2 mg/L 2,4-D concentration) as well as higher number of embryos per explants (~7 at the same 2,4-D concentration) compared to its either parents. The germinated embryos, after culturing on GA3 supplemented medium, were transferred to hormone free ½ SH medium to support the continued growth and kept until nutrient depletion induced senescence (NuDIS) of leaf defoliation occurred (4 months). By that time, thickened tap roots with well-developed lateral roots and dormant buds were obtained. All Pgq tap roots pretreated with 20 mg/L GA3 for at least a week produced new shoots after soil transfer. We selected the discriminatory RAPD and ISSR markers to find the interspecific ginseng hybrid among its parents. The F1 hybrid (Pgq) contained species specific 2 ginsenosides (ginsenoside Rf in P. ginseng and pseudoginsenosides F11 in P. quinquefolius), and higher amount of other ginsenosides than its parents. Conclusion: Micropropagation of interspecific hybrid ginseng can give an opportunity for continuous production of plants. Keywords: hybrid, micropropagation, Panax ginseng, Panax quinquefolius, somatic embryogenesishttp://www.sciencedirect.com/science/article/pii/S1226845316302366 |
| spellingShingle | Jong Youn Kim Prakash Babu Adhikari Chang Ho Ahn Dong Hwi Kim Young Chang Kim Jung Yeon Han Subramanyam Kondeti Yong Eui Choi High frequency somatic embryogenesis and plant regeneration of interspecific ginseng hybrid between Panax ginseng and Panax quinquefolius Journal of Ginseng Research |
| title | High frequency somatic embryogenesis and plant regeneration of interspecific ginseng hybrid between Panax ginseng and Panax quinquefolius |
| title_full | High frequency somatic embryogenesis and plant regeneration of interspecific ginseng hybrid between Panax ginseng and Panax quinquefolius |
| title_fullStr | High frequency somatic embryogenesis and plant regeneration of interspecific ginseng hybrid between Panax ginseng and Panax quinquefolius |
| title_full_unstemmed | High frequency somatic embryogenesis and plant regeneration of interspecific ginseng hybrid between Panax ginseng and Panax quinquefolius |
| title_short | High frequency somatic embryogenesis and plant regeneration of interspecific ginseng hybrid between Panax ginseng and Panax quinquefolius |
| title_sort | high frequency somatic embryogenesis and plant regeneration of interspecific ginseng hybrid between panax ginseng and panax quinquefolius |
| url | http://www.sciencedirect.com/science/article/pii/S1226845316302366 |
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