Characterization, Localization and Patterning of Lymphatics and Blood Vessels in Oral Squamous Cell Carcinoma: A Comparative Study Using D2-40 and CD-34 IHC Marker
Objectives: Lymphatic metastasis has always been regarded as a major prognostic indicator for disease progression and as a guide for therapeutic strategies to oral squamous cell carcinoma (OSCC). Differentiating lymphatic vessels from blood vessels is difficult, partly due to lack of specific me...
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| Format: | Article |
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JCDR Research and Publications Private Limited
2014-10-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/5072/10311_CE(Ra)_F(Sh)_PF1(AJAK)_PFA(AK)_PF2(PAG).pdf |
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| author | Deshant Agarwal Nilesh Pardhe Manas Bajpai Shailendra Gupta Nikunj Mathur S S Vanaki R S Puranik Manoj Mittal |
| author_facet | Deshant Agarwal Nilesh Pardhe Manas Bajpai Shailendra Gupta Nikunj Mathur S S Vanaki R S Puranik Manoj Mittal |
| author_sort | Deshant Agarwal |
| collection | DOAJ |
| description | Objectives: Lymphatic metastasis has always been regarded
as a major prognostic indicator for disease progression and
as a guide for therapeutic strategies to oral squamous cell
carcinoma (OSCC). Differentiating lymphatic vessels from
blood vessels is difficult, partly due to lack of specific method
for identifying lymphatics. A new lymphatic vessel reactive
antibody D2-40 has been introduced recently. Here we examined
immunohistochemical localization of lymphatic vessels and
blood vessels using D2-40 and CD-34 respectively in different
histological grades of OSCC. Their expression in intra-tumoural
and peri-tumoural region was also compared.
Materials and Methods: Forty two formalin-fixed paraffinembedded tissue blocks of excised specimens of OSCC were
immunohistochemically evaluated using D2-40 and CD-34
antibodies. Lymphatic vessel density (LVD) (D2-40 positivity)
and micro vessel density (MVD) (CD34 positivity) in both
intratumoural and peritumoural areas were assessed by hot
spot method.
Results: Regardless of histopathological differentiation,
LVD and MVD in peritumoural areas were found greater than
intratumoural areas (p>0.05). Interestingly, other than lymphatic
vessels, D2-40 positivity was also detected in tumour cells
as well as in basal layer of epithelium adjacent to OSCC. Two
patterns of distribution of CD34 positive vessel - circumscribing
type and penetrating type were also observed in the cancer
nest area.
Conclusion: D2-40 can be used as a marker to differentiate
lymphatic vessels from blood vessels. Lymphatic and blood
vessel proliferation might be much more extensive in the
peritumoural area. D2-40 expression in epithelium adjacent
to tumour indicates its role in the process of differentiation.
Further, its expression in potential malignant disorder may
provide better insight in predicting prognosis and pathogenesis
of these lesions. |
| first_indexed | 2024-12-17T20:21:17Z |
| format | Article |
| id | doaj.art-27bbb6c9df4a481f8ae41fc923c9ae85 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-17T20:21:17Z |
| publishDate | 2014-10-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-27bbb6c9df4a481f8ae41fc923c9ae852022-12-21T21:33:56ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2014-10-01810ZC86ZC8910.7860/JCDR/2014/10311.5072Characterization, Localization and Patterning of Lymphatics and Blood Vessels in Oral Squamous Cell Carcinoma: A Comparative Study Using D2-40 and CD-34 IHC MarkerDeshant Agarwal0Nilesh Pardhe1Manas Bajpai2Shailendra Gupta3Nikunj Mathur4S S Vanaki5R S Puranik6Manoj Mittal7Assistant Professor, Department of Oral and Maxillofacial Pathology, NIMS Dental College and Hospital, Jaipur, India.Professor & Head, Department of Oral & Maxillofacial Pathology, NIMS Dental College & Hospital, Jaipur, Rajasthan, India.Assistant Professor, Department of Oral and Maxillofacial Pathology, NIMS Dental College and Hospital, Jaipur, India.Professor & Head, Department of Conservative Dentistry and Endodontics, RKDF Dental College, Bhopal, India.Assistant Professor, Department of Oral Medicine & Radiology, Government Dental College, Jaipur, India.Professor and Head, Department of Oral and Maxillofacial Pathology, PMNM Dental College and Hospital, Bagalkot, Karnataka, India.Professor, Department of Oral and Maxillofacial Pathology, PMNM Dental College and Hospital, Bagalkot, Karnataka, India.Professor & Head, Department of Periodontics, RKDF Dental College, Bhopal, India.Objectives: Lymphatic metastasis has always been regarded as a major prognostic indicator for disease progression and as a guide for therapeutic strategies to oral squamous cell carcinoma (OSCC). Differentiating lymphatic vessels from blood vessels is difficult, partly due to lack of specific method for identifying lymphatics. A new lymphatic vessel reactive antibody D2-40 has been introduced recently. Here we examined immunohistochemical localization of lymphatic vessels and blood vessels using D2-40 and CD-34 respectively in different histological grades of OSCC. Their expression in intra-tumoural and peri-tumoural region was also compared. Materials and Methods: Forty two formalin-fixed paraffinembedded tissue blocks of excised specimens of OSCC were immunohistochemically evaluated using D2-40 and CD-34 antibodies. Lymphatic vessel density (LVD) (D2-40 positivity) and micro vessel density (MVD) (CD34 positivity) in both intratumoural and peritumoural areas were assessed by hot spot method. Results: Regardless of histopathological differentiation, LVD and MVD in peritumoural areas were found greater than intratumoural areas (p>0.05). Interestingly, other than lymphatic vessels, D2-40 positivity was also detected in tumour cells as well as in basal layer of epithelium adjacent to OSCC. Two patterns of distribution of CD34 positive vessel - circumscribing type and penetrating type were also observed in the cancer nest area. Conclusion: D2-40 can be used as a marker to differentiate lymphatic vessels from blood vessels. Lymphatic and blood vessel proliferation might be much more extensive in the peritumoural area. D2-40 expression in epithelium adjacent to tumour indicates its role in the process of differentiation. Further, its expression in potential malignant disorder may provide better insight in predicting prognosis and pathogenesis of these lesions.https://jcdr.net/articles/PDF/5072/10311_CE(Ra)_F(Sh)_PF1(AJAK)_PFA(AK)_PF2(PAG).pdfendothelial cellsintratumoural areametastasisperitumoural areaprognosis |
| spellingShingle | Deshant Agarwal Nilesh Pardhe Manas Bajpai Shailendra Gupta Nikunj Mathur S S Vanaki R S Puranik Manoj Mittal Characterization, Localization and Patterning of Lymphatics and Blood Vessels in Oral Squamous Cell Carcinoma: A Comparative Study Using D2-40 and CD-34 IHC Marker Journal of Clinical and Diagnostic Research endothelial cells intratumoural area metastasis peritumoural area prognosis |
| title | Characterization, Localization and Patterning of Lymphatics and Blood Vessels in Oral Squamous Cell Carcinoma: A Comparative Study Using D2-40 and CD-34 IHC Marker |
| title_full | Characterization, Localization and Patterning of Lymphatics and Blood Vessels in Oral Squamous Cell Carcinoma: A Comparative Study Using D2-40 and CD-34 IHC Marker |
| title_fullStr | Characterization, Localization and Patterning of Lymphatics and Blood Vessels in Oral Squamous Cell Carcinoma: A Comparative Study Using D2-40 and CD-34 IHC Marker |
| title_full_unstemmed | Characterization, Localization and Patterning of Lymphatics and Blood Vessels in Oral Squamous Cell Carcinoma: A Comparative Study Using D2-40 and CD-34 IHC Marker |
| title_short | Characterization, Localization and Patterning of Lymphatics and Blood Vessels in Oral Squamous Cell Carcinoma: A Comparative Study Using D2-40 and CD-34 IHC Marker |
| title_sort | characterization localization and patterning of lymphatics and blood vessels in oral squamous cell carcinoma a comparative study using d2 40 and cd 34 ihc marker |
| topic | endothelial cells intratumoural area metastasis peritumoural area prognosis |
| url | https://jcdr.net/articles/PDF/5072/10311_CE(Ra)_F(Sh)_PF1(AJAK)_PFA(AK)_PF2(PAG).pdf |
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