Characterization of the pathogenicity of a Bacillus cereus isolate from the Mariana Trench
Bacillus cereus is an important opportunistic pathogen widely distributed in the environment. In this study, we reported the isolation and characterization of a B. cereus isolate, MB1, from the Challenger Deep of the Mariana Trench. MB1 is aerobic, motile, and able to form endospores. It possesses 5...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Virulence |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2022.2088641 |
| _version_ | 1818251904021430272 |
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| author | Yujian Wang Jian Zhang Zihao Yuan Li Sun |
| author_facet | Yujian Wang Jian Zhang Zihao Yuan Li Sun |
| author_sort | Yujian Wang |
| collection | DOAJ |
| description | Bacillus cereus is an important opportunistic pathogen widely distributed in the environment. In this study, we reported the isolation and characterization of a B. cereus isolate, MB1, from the Challenger Deep of the Mariana Trench. MB1 is aerobic, motile, and able to form endospores. It possesses 5966 genes distributed on a circular chromosome and two plasmids. The MB1 genome contains 14 sets of 23S, 5S, and 16S ribosomal RNA operons, 106 tRNA genes, 4 sRNA genes, 12 genomic islands, 13 prophages, and 302 putative virulence genes, including enterotoxins and cytolysins. Infection studies showed that MB1 was able to cause acute and lethal infection in fish and mice, and was highly toxic to mammalian cells. MB1 induced, in a dose-dependent manner, pyroptotic cell death, characterized by activation of caspase-1, cleavage of gasdermin D, and release of IL-1β and IL-18. MB1 spores exhibited swimming and haemolytic capacity, but were severely attenuated in pathogenicity, which, however, was regained to the full extent when the spores germinated under suitable conditions. Taken together, these results provide new insights into the biological and pathogenic mechanism of deep sea B. cereus. |
| first_indexed | 2024-12-12T16:15:41Z |
| format | Article |
| id | doaj.art-27bfc7f796a44153b1f830c0f90d303d |
| institution | Directory Open Access Journal |
| issn | 2150-5594 2150-5608 |
| language | English |
| last_indexed | 2024-12-12T16:15:41Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj.art-27bfc7f796a44153b1f830c0f90d303d2022-12-22T00:19:06ZengTaylor & Francis GroupVirulence2150-55942150-56082022-12-011311062107510.1080/21505594.2022.2088641Characterization of the pathogenicity of a Bacillus cereus isolate from the Mariana TrenchYujian Wang0Jian Zhang1Zihao Yuan2Li Sun3CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaSchool of Ocean, Yan tai University, Yantai, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, ChinaBacillus cereus is an important opportunistic pathogen widely distributed in the environment. In this study, we reported the isolation and characterization of a B. cereus isolate, MB1, from the Challenger Deep of the Mariana Trench. MB1 is aerobic, motile, and able to form endospores. It possesses 5966 genes distributed on a circular chromosome and two plasmids. The MB1 genome contains 14 sets of 23S, 5S, and 16S ribosomal RNA operons, 106 tRNA genes, 4 sRNA genes, 12 genomic islands, 13 prophages, and 302 putative virulence genes, including enterotoxins and cytolysins. Infection studies showed that MB1 was able to cause acute and lethal infection in fish and mice, and was highly toxic to mammalian cells. MB1 induced, in a dose-dependent manner, pyroptotic cell death, characterized by activation of caspase-1, cleavage of gasdermin D, and release of IL-1β and IL-18. MB1 spores exhibited swimming and haemolytic capacity, but were severely attenuated in pathogenicity, which, however, was regained to the full extent when the spores germinated under suitable conditions. Taken together, these results provide new insights into the biological and pathogenic mechanism of deep sea B. cereus.https://www.tandfonline.com/doi/10.1080/21505594.2022.2088641Bacillus cereusdeep seapathogenicitycytotoxicityspore |
| spellingShingle | Yujian Wang Jian Zhang Zihao Yuan Li Sun Characterization of the pathogenicity of a Bacillus cereus isolate from the Mariana Trench Virulence Bacillus cereus deep sea pathogenicity cytotoxicity spore |
| title | Characterization of the pathogenicity of a Bacillus cereus isolate from the Mariana Trench |
| title_full | Characterization of the pathogenicity of a Bacillus cereus isolate from the Mariana Trench |
| title_fullStr | Characterization of the pathogenicity of a Bacillus cereus isolate from the Mariana Trench |
| title_full_unstemmed | Characterization of the pathogenicity of a Bacillus cereus isolate from the Mariana Trench |
| title_short | Characterization of the pathogenicity of a Bacillus cereus isolate from the Mariana Trench |
| title_sort | characterization of the pathogenicity of a bacillus cereus isolate from the mariana trench |
| topic | Bacillus cereus deep sea pathogenicity cytotoxicity spore |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2022.2088641 |
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