Identification by molecular techniques of halophilic bacteria producing important enzymes from pristine area in Campeche, Mexico
Abstract Isla Arena is located in the coordinate 20° 70´ N - 90° 45´ W, from Campeche, Mexico. In these estuaries, the ocean mixes with fresh water, and ecosystems are concentrated where petenes and pink flamingos proliferate. Crustaceans and mollusks abound in the sea. Despite its enormous marine w...
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
2021-09-01
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| Series: | Brazilian Journal of Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100213&tlng=en |
| _version_ | 1818951345487478784 |
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| author | L. A. Can-Herrera C. D. Gutierrez-Canul M. A. A. Dzul-Cervantes O. F. Pacheco-Salazar J. D. Chi-Cortez L. Saenz Carbonell |
| author_facet | L. A. Can-Herrera C. D. Gutierrez-Canul M. A. A. Dzul-Cervantes O. F. Pacheco-Salazar J. D. Chi-Cortez L. Saenz Carbonell |
| author_sort | L. A. Can-Herrera |
| collection | DOAJ |
| description | Abstract Isla Arena is located in the coordinate 20° 70´ N - 90° 45´ W, from Campeche, Mexico. In these estuaries, the ocean mixes with fresh water, and ecosystems are concentrated where petenes and pink flamingos proliferate. Crustaceans and mollusks abound in the sea. Despite its enormous marine wealth, there are no studies carried out on which halophilic microorganisms are present in these waters. In this work, the diversity and structure of the microbial community was investigated through a metagenomics approach and corroborated for sequencing of 16S rRNA genes. It was found that the phylum Fimicutes predominates with more than 50%, in almost the same proportion of the class Bacilli and with almost 41% of relative abundance of the order Bacillales. The sequencing results showed that one of the samples presented a high percentage of similarity (99.75%) using the Nucleotide BLAST program with a peculiar microorganism: Bacillus subtilis. This microorganism is one of the best characterized bacteria among the gram-positive ones. Our results demonstrate that B. subtilis can be an efficient source of proteases, lipases and cellulases, from halophilic microbial communities located in poorly explored areas. |
| first_indexed | 2024-12-20T09:33:00Z |
| format | Article |
| id | doaj.art-27c2e05e2cf147bca0ad3096cdc91017 |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-12-20T09:33:00Z |
| publishDate | 2021-09-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-27c2e05e2cf147bca0ad3096cdc910172022-12-21T19:45:03ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752021-09-018310.1590/1519-6984.246038Identification by molecular techniques of halophilic bacteria producing important enzymes from pristine area in Campeche, MexicoL. A. Can-Herrerahttps://orcid.org/0000-0001-7926-0320C. D. Gutierrez-Canulhttps://orcid.org/0000-0003-3067-8202M. A. A. Dzul-Cervanteshttps://orcid.org/0000-0002-9095-7281O. F. Pacheco-Salazarhttps://orcid.org/0000-0001-5677-0037J. D. Chi-Cortezhttps://orcid.org/0000-0002-0849-3490L. Saenz Carbonellhttps://orcid.org/0000-0003-0739-2206Abstract Isla Arena is located in the coordinate 20° 70´ N - 90° 45´ W, from Campeche, Mexico. In these estuaries, the ocean mixes with fresh water, and ecosystems are concentrated where petenes and pink flamingos proliferate. Crustaceans and mollusks abound in the sea. Despite its enormous marine wealth, there are no studies carried out on which halophilic microorganisms are present in these waters. In this work, the diversity and structure of the microbial community was investigated through a metagenomics approach and corroborated for sequencing of 16S rRNA genes. It was found that the phylum Fimicutes predominates with more than 50%, in almost the same proportion of the class Bacilli and with almost 41% of relative abundance of the order Bacillales. The sequencing results showed that one of the samples presented a high percentage of similarity (99.75%) using the Nucleotide BLAST program with a peculiar microorganism: Bacillus subtilis. This microorganism is one of the best characterized bacteria among the gram-positive ones. Our results demonstrate that B. subtilis can be an efficient source of proteases, lipases and cellulases, from halophilic microbial communities located in poorly explored areas.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100213&tlng=enhalophilic bacteriaBacillus subtilisproteaseslipasescellulases |
| spellingShingle | L. A. Can-Herrera C. D. Gutierrez-Canul M. A. A. Dzul-Cervantes O. F. Pacheco-Salazar J. D. Chi-Cortez L. Saenz Carbonell Identification by molecular techniques of halophilic bacteria producing important enzymes from pristine area in Campeche, Mexico Brazilian Journal of Biology halophilic bacteria Bacillus subtilis proteases lipases cellulases |
| title | Identification by molecular techniques of halophilic bacteria producing important enzymes from pristine area in Campeche, Mexico |
| title_full | Identification by molecular techniques of halophilic bacteria producing important enzymes from pristine area in Campeche, Mexico |
| title_fullStr | Identification by molecular techniques of halophilic bacteria producing important enzymes from pristine area in Campeche, Mexico |
| title_full_unstemmed | Identification by molecular techniques of halophilic bacteria producing important enzymes from pristine area in Campeche, Mexico |
| title_short | Identification by molecular techniques of halophilic bacteria producing important enzymes from pristine area in Campeche, Mexico |
| title_sort | identification by molecular techniques of halophilic bacteria producing important enzymes from pristine area in campeche mexico |
| topic | halophilic bacteria Bacillus subtilis proteases lipases cellulases |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100213&tlng=en |
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