Gene expression changes of single skeletal muscle fibers in response to modulation of the mitochondrial calcium uniporter (MCU)
The mitochondrial calcium uniporter (MCU) gene codifies for the inner mitochondrial membrane (IMM) channel responsible for mitochondrial Ca2+ uptake. Cytosolic Ca2+ transients are involved in sarcomere contraction through cycles of release and storage in the sarcoplasmic reticulum. In addition cytos...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-09-01
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| Series: | Genomics Data |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213596015000860 |
| _version_ | 1818311444469383168 |
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| author | Francesco Chemello Cristina Mammucari Gaia Gherardi Rosario Rizzuto Gerolamo Lanfranchi Stefano Cagnin |
| author_facet | Francesco Chemello Cristina Mammucari Gaia Gherardi Rosario Rizzuto Gerolamo Lanfranchi Stefano Cagnin |
| author_sort | Francesco Chemello |
| collection | DOAJ |
| description | The mitochondrial calcium uniporter (MCU) gene codifies for the inner mitochondrial membrane (IMM) channel responsible for mitochondrial Ca2+ uptake. Cytosolic Ca2+ transients are involved in sarcomere contraction through cycles of release and storage in the sarcoplasmic reticulum. In addition cytosolic Ca2+ regulates various signaling cascades that eventually lead to gene expression reprogramming. Mitochondria are strategically placed in close contact with the ER/SR, thus cytosolic Ca2+ transients elicit large increases in the [Ca2+] of the mitochondrial matrix ([Ca2+]mt). Mitochondrial Ca2+ uptake regulates energy production and cell survival. In addition, we recently showed that MCU-dependent mitochondrial Ca2+ uptake controls skeletal muscle trophism. In the same report, we dissected the effects of MCU-dependent mitochondrial Ca2+ uptake on gene expression through microarray gene expression analysis upon modulation of MCU expression by in vivo AAV infection. Analyses were performed on single skeletal muscle fibers at two time points (7 and 14 days post-AAV injection). Raw and normalized data are available on the GEO database (http://www.ncbi.nlm.nih.gov/geo/) (GSE60931). |
| first_indexed | 2024-12-13T08:02:03Z |
| format | Article |
| id | doaj.art-27c38020a3bb46d49bec68f3a7d84974 |
| institution | Directory Open Access Journal |
| issn | 2213-5960 |
| language | English |
| last_indexed | 2024-12-13T08:02:03Z |
| publishDate | 2015-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Genomics Data |
| spelling | doaj.art-27c38020a3bb46d49bec68f3a7d849742022-12-21T23:54:23ZengElsevierGenomics Data2213-59602015-09-015C646710.1016/j.gdata.2015.05.023Gene expression changes of single skeletal muscle fibers in response to modulation of the mitochondrial calcium uniporter (MCU)Francesco Chemello0Cristina Mammucari1Gaia Gherardi2Rosario Rizzuto3Gerolamo Lanfranchi4Stefano Cagnin5Department of Biology, University of Padua, Padua 35131, ItalyDepartment of Biomedical Sciences, University of Padua, Padua 35131, ItalyDepartment of Biomedical Sciences, University of Padua, Padua 35131, ItalyDepartment of Biomedical Sciences, University of Padua, Padua 35131, ItalyDepartment of Biology, University of Padua, Padua 35131, ItalyDepartment of Biology, University of Padua, Padua 35131, ItalyThe mitochondrial calcium uniporter (MCU) gene codifies for the inner mitochondrial membrane (IMM) channel responsible for mitochondrial Ca2+ uptake. Cytosolic Ca2+ transients are involved in sarcomere contraction through cycles of release and storage in the sarcoplasmic reticulum. In addition cytosolic Ca2+ regulates various signaling cascades that eventually lead to gene expression reprogramming. Mitochondria are strategically placed in close contact with the ER/SR, thus cytosolic Ca2+ transients elicit large increases in the [Ca2+] of the mitochondrial matrix ([Ca2+]mt). Mitochondrial Ca2+ uptake regulates energy production and cell survival. In addition, we recently showed that MCU-dependent mitochondrial Ca2+ uptake controls skeletal muscle trophism. In the same report, we dissected the effects of MCU-dependent mitochondrial Ca2+ uptake on gene expression through microarray gene expression analysis upon modulation of MCU expression by in vivo AAV infection. Analyses were performed on single skeletal muscle fibers at two time points (7 and 14 days post-AAV injection). Raw and normalized data are available on the GEO database (http://www.ncbi.nlm.nih.gov/geo/) (GSE60931).http://www.sciencedirect.com/science/article/pii/S2213596015000860 |
| spellingShingle | Francesco Chemello Cristina Mammucari Gaia Gherardi Rosario Rizzuto Gerolamo Lanfranchi Stefano Cagnin Gene expression changes of single skeletal muscle fibers in response to modulation of the mitochondrial calcium uniporter (MCU) Genomics Data |
| title | Gene expression changes of single skeletal muscle fibers in response to modulation of the mitochondrial calcium uniporter (MCU) |
| title_full | Gene expression changes of single skeletal muscle fibers in response to modulation of the mitochondrial calcium uniporter (MCU) |
| title_fullStr | Gene expression changes of single skeletal muscle fibers in response to modulation of the mitochondrial calcium uniporter (MCU) |
| title_full_unstemmed | Gene expression changes of single skeletal muscle fibers in response to modulation of the mitochondrial calcium uniporter (MCU) |
| title_short | Gene expression changes of single skeletal muscle fibers in response to modulation of the mitochondrial calcium uniporter (MCU) |
| title_sort | gene expression changes of single skeletal muscle fibers in response to modulation of the mitochondrial calcium uniporter mcu |
| url | http://www.sciencedirect.com/science/article/pii/S2213596015000860 |
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