Transcriptome and physiological analyses for revealing genes involved in wheat response to endoplasmic reticulum stress
Abstract Background Wheat production is largely restricted by adverse environmental stresses. Under many undesirable conditions, endoplasmic reticulum (ER) stress can be induced. However, the physiological and molecular responses of wheat to ER stress remain poorly understood. We used dithiothreitol...
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| Format: | Article |
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BMC
2019-05-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-019-1798-7 |
| _version_ | 1811206636689162240 |
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| author | Xing Yu Tanchun Wang Meichen Zhu Liting Zhang Fengzhi Zhang Enen Jing Yongzhe Ren Zhiqiang Wang Zeyu Xin Tongbao Lin |
| author_facet | Xing Yu Tanchun Wang Meichen Zhu Liting Zhang Fengzhi Zhang Enen Jing Yongzhe Ren Zhiqiang Wang Zeyu Xin Tongbao Lin |
| author_sort | Xing Yu |
| collection | DOAJ |
| description | Abstract Background Wheat production is largely restricted by adverse environmental stresses. Under many undesirable conditions, endoplasmic reticulum (ER) stress can be induced. However, the physiological and molecular responses of wheat to ER stress remain poorly understood. We used dithiothreitol (DTT) and tauroursodeoxycholic acid (TUDCA) to induce or suppress ER stress in wheat cells, respectively, with the aim to reveal the molecular background of ER stress responses using a combined approach of transcriptional profiling and morpho-physiological characterization. Methods To understand the mechanism of wheat response to ER stress, three wheat cultivars were used in our pre-experiments. Among them, the cultivar with a moderate stress tolerance, Yunong211 was used in the following experiments. We used DTT (7.5 mM) to induce ER stress and TUDCA (25 μg·mL− 1) to suppress the stress. Under three treatment groups (Control, DTT and DTT + TUDCA), we firstly monitored the morphological, physiological and cytological changes of wheat seedlings. Then we collected leaf samples from each group for RNA extraction, library construction and RNA sequencing on an Illumina Hiseq platform. The sequencing data was then validated by qRT-PCR. Results Morpho-physiological results showed DTT significantly reduced plant height and biomass, decreased contents of chlorophyll and water, increased electrolyte leakage rate and antioxidant enzymes activity, and accelerated the cell death ratio, whereas these changes were all remarkably alleviated after TUDCA co-treatment. Therefore, RNA sequencing was performed to determine the genes involved in regulating wheat response to stress. Transcriptomic analysis revealed that 8204 genes were differentially expressed in three treatment groups. Among these genes, 158 photosynthesis-related genes, 42 antioxidant enzyme genes, 318 plant hormone-related genes and 457 transcription factors (TFs) may play vital roles in regulating wheat response to ER stress. Based on the comprehensive analysis, we propose a hypothetical model to elucidate possible mechanisms of how plants adapt to environmental stresses. Conclusions We identified several important genes that may play vital roles in wheat responding to ER stress. This work should lay the foundations of future studies in plant response to environmental stresses. |
| first_indexed | 2024-04-12T03:50:30Z |
| format | Article |
| id | doaj.art-27c61ecb6c9a47498e2a5a8eca6b1414 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-12T03:50:30Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
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| series | BMC Plant Biology |
| spelling | doaj.art-27c61ecb6c9a47498e2a5a8eca6b14142022-12-22T03:48:59ZengBMCBMC Plant Biology1471-22292019-05-0119112210.1186/s12870-019-1798-7Transcriptome and physiological analyses for revealing genes involved in wheat response to endoplasmic reticulum stressXing Yu0Tanchun Wang1Meichen Zhu2Liting Zhang3Fengzhi Zhang4Enen Jing5Yongzhe Ren6Zhiqiang Wang7Zeyu Xin8Tongbao Lin9College of Agronomy, Henan Agricultural UniversityDepartment of Basic Biomedical Sciences, Touro College of Osteopathic Medicine – MiddletownCollege of Agronomy, Henan Agricultural UniversityCollege of Agronomy, Henan Agricultural UniversityCollege of Agronomy, Henan Agricultural UniversityCollege of Agronomy, Henan Agricultural UniversityCollege of Agronomy, Henan Agricultural UniversityCollege of Agronomy, Henan Agricultural UniversityCollege of Agronomy, Henan Agricultural UniversityCollege of Agronomy, Henan Agricultural UniversityAbstract Background Wheat production is largely restricted by adverse environmental stresses. Under many undesirable conditions, endoplasmic reticulum (ER) stress can be induced. However, the physiological and molecular responses of wheat to ER stress remain poorly understood. We used dithiothreitol (DTT) and tauroursodeoxycholic acid (TUDCA) to induce or suppress ER stress in wheat cells, respectively, with the aim to reveal the molecular background of ER stress responses using a combined approach of transcriptional profiling and morpho-physiological characterization. Methods To understand the mechanism of wheat response to ER stress, three wheat cultivars were used in our pre-experiments. Among them, the cultivar with a moderate stress tolerance, Yunong211 was used in the following experiments. We used DTT (7.5 mM) to induce ER stress and TUDCA (25 μg·mL− 1) to suppress the stress. Under three treatment groups (Control, DTT and DTT + TUDCA), we firstly monitored the morphological, physiological and cytological changes of wheat seedlings. Then we collected leaf samples from each group for RNA extraction, library construction and RNA sequencing on an Illumina Hiseq platform. The sequencing data was then validated by qRT-PCR. Results Morpho-physiological results showed DTT significantly reduced plant height and biomass, decreased contents of chlorophyll and water, increased electrolyte leakage rate and antioxidant enzymes activity, and accelerated the cell death ratio, whereas these changes were all remarkably alleviated after TUDCA co-treatment. Therefore, RNA sequencing was performed to determine the genes involved in regulating wheat response to stress. Transcriptomic analysis revealed that 8204 genes were differentially expressed in three treatment groups. Among these genes, 158 photosynthesis-related genes, 42 antioxidant enzyme genes, 318 plant hormone-related genes and 457 transcription factors (TFs) may play vital roles in regulating wheat response to ER stress. Based on the comprehensive analysis, we propose a hypothetical model to elucidate possible mechanisms of how plants adapt to environmental stresses. Conclusions We identified several important genes that may play vital roles in wheat responding to ER stress. This work should lay the foundations of future studies in plant response to environmental stresses.http://link.springer.com/article/10.1186/s12870-019-1798-7Antioxidant enzymesDTTTUDCARNA-seqTranscription factorsChlorophyll |
| spellingShingle | Xing Yu Tanchun Wang Meichen Zhu Liting Zhang Fengzhi Zhang Enen Jing Yongzhe Ren Zhiqiang Wang Zeyu Xin Tongbao Lin Transcriptome and physiological analyses for revealing genes involved in wheat response to endoplasmic reticulum stress BMC Plant Biology Antioxidant enzymes DTT TUDCA RNA-seq Transcription factors Chlorophyll |
| title | Transcriptome and physiological analyses for revealing genes involved in wheat response to endoplasmic reticulum stress |
| title_full | Transcriptome and physiological analyses for revealing genes involved in wheat response to endoplasmic reticulum stress |
| title_fullStr | Transcriptome and physiological analyses for revealing genes involved in wheat response to endoplasmic reticulum stress |
| title_full_unstemmed | Transcriptome and physiological analyses for revealing genes involved in wheat response to endoplasmic reticulum stress |
| title_short | Transcriptome and physiological analyses for revealing genes involved in wheat response to endoplasmic reticulum stress |
| title_sort | transcriptome and physiological analyses for revealing genes involved in wheat response to endoplasmic reticulum stress |
| topic | Antioxidant enzymes DTT TUDCA RNA-seq Transcription factors Chlorophyll |
| url | http://link.springer.com/article/10.1186/s12870-019-1798-7 |
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