Prophage-Derived Regions in <i>Curtobacterium</i> Genomes: Good Things, Small Packages
<i>Curtobacterium</i> is a genus of Gram-positive bacteria within the order <i>Actinomycetales</i>. Some <i>Curtobacterium</i> species (<i>C. flaccumfaciens</i>, <i>C. plantarum</i>) are harmful pathogens of agricultural crops such as soybe...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/2/1586 |
| Summary: | <i>Curtobacterium</i> is a genus of Gram-positive bacteria within the order <i>Actinomycetales</i>. Some <i>Curtobacterium</i> species (<i>C. flaccumfaciens</i>, <i>C. plantarum</i>) are harmful pathogens of agricultural crops such as soybean, dry beans, peas, sugar beet and beetroot, which occur throughout the world. Bacteriophages (bacterial viruses) are considered to be potential curative agents to control the spread of harmful bacteria. Temperate bacteriophages integrate their genomes into bacterial chromosomes (prophages), sometimes substantially influencing bacterial lifestyle and pathogenicity. About 200 publicly available genomes of <i>Curtobacterium</i> species, including environmental metagenomic sequences, were inspected for the presence of sequences of possible prophage origin using bioinformatic methods. The comparison of the search results with several ubiquitous bacterial groups showed the relatively low level of the presence of prophage traces in <i>Curtobacterium</i> genomes. Genomic and phylogenetic analyses were undertaken for the evaluation of the evolutionary and taxonomic positioning of predicted prophages. The analyses indicated the relatedness of <i>Curtobacterium</i> prophage-derived sequences with temperate actinophages of siphoviral morphology. In most cases, the predicted prophages can represent novel phage taxa not described previously. One of the predicted temperate phages was induced from the <i>Curtobacterium</i> genome. Bioinformatic analysis of the modelled proteins encoded in prophage-derived regions led to the discovery of some 100 putative glycopolymer-degrading enzymes that contained enzymatic domains with predicted cell-wall- and cell-envelope-degrading activity; these included glycosidases and peptidases. These proteins can be considered for the experimental design of new antibacterials against <i>Curtobacterium</i> phytopathogens. |
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| ISSN: | 1661-6596 1422-0067 |