A Technical System for the Large-Scale Application of Metabolites From Paecilomyces variotii SJ1 in Agriculture
Compared with endophytes, metabolites from endophytes (MEs) have great potential in agriculture. However, a technique for industrializing the production of MEs is still scarce. Moreover, the establishment of effective methods for evaluating the quality of MEs is hampered by the fact that some compou...
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| Format: | Article |
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Frontiers Media S.A.
2021-05-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.671879/full |
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| author | Qingbin Wang Qingbin Wang Chune Peng Liran Shi Zhiguang Liu Dafa Zhou Hui Meng Hongling Zhao Fuchuan Li Min Zhang |
| author_facet | Qingbin Wang Qingbin Wang Chune Peng Liran Shi Zhiguang Liu Dafa Zhou Hui Meng Hongling Zhao Fuchuan Li Min Zhang |
| author_sort | Qingbin Wang |
| collection | DOAJ |
| description | Compared with endophytes, metabolites from endophytes (MEs) have great potential in agriculture. However, a technique for industrializing the production of MEs is still scarce. Moreover, the establishment of effective methods for evaluating the quality of MEs is hampered by the fact that some compounds with beneficial effects on crops have not been clearly identified. Herein, a system was established for the production, quality control and application of MEs by using the extract from Paecilomyces variotii SJ1 (ZNC). First, the extraction conditions of ZNC were optimized through response surface methodology, after which each batch (500 L) met the consumption requirements of crops in 7,467 hectares. Then, chromatographic fingerprinting and enzyme-linked immunosorbent assay were applied to evaluate the similarity and specificity of unknown effective components in ZNC, ensuring a similarity of more than 90% and a quantitative accuracy of greater than 99.9% for the products from different batches. Finally, the bioactivity of industrially produced ZNC was evaluated in the field, and it significantly increased the potato yields by 4.4–10.8%. Overall, we have established a practical technical system for the large-scale application of ZNC in agriculture. |
| first_indexed | 2024-12-17T23:48:43Z |
| format | Article |
| id | doaj.art-27c6e6126ae24f7385537e75a2a3f018 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-17T23:48:43Z |
| publishDate | 2021-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-27c6e6126ae24f7385537e75a2a3f0182022-12-21T21:28:14ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-05-01910.3389/fbioe.2021.671879671879A Technical System for the Large-Scale Application of Metabolites From Paecilomyces variotii SJ1 in AgricultureQingbin Wang0Qingbin Wang1Chune Peng2Liran Shi3Zhiguang Liu4Dafa Zhou5Hui Meng6Hongling Zhao7Fuchuan Li8Min Zhang9National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, National Engineering and Technology Research Center for Slow and Controlled Release Fertilizers, Shandong Agricultural University, Tai’an, ChinaShandong Pengbo Biotechnology Co., Ltd., Tai’an, ChinaState Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an, ChinaShandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, National Glycoengineering Research Center, Shandong University, Qingdao, ChinaNational Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, National Engineering and Technology Research Center for Slow and Controlled Release Fertilizers, Shandong Agricultural University, Tai’an, ChinaState Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an, ChinaShandong Pengbo Biotechnology Co., Ltd., Tai’an, ChinaShandong Pengbo Biotechnology Co., Ltd., Tai’an, ChinaState Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an, ChinaNational Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Resources and Environment, National Engineering and Technology Research Center for Slow and Controlled Release Fertilizers, Shandong Agricultural University, Tai’an, ChinaCompared with endophytes, metabolites from endophytes (MEs) have great potential in agriculture. However, a technique for industrializing the production of MEs is still scarce. Moreover, the establishment of effective methods for evaluating the quality of MEs is hampered by the fact that some compounds with beneficial effects on crops have not been clearly identified. Herein, a system was established for the production, quality control and application of MEs by using the extract from Paecilomyces variotii SJ1 (ZNC). First, the extraction conditions of ZNC were optimized through response surface methodology, after which each batch (500 L) met the consumption requirements of crops in 7,467 hectares. Then, chromatographic fingerprinting and enzyme-linked immunosorbent assay were applied to evaluate the similarity and specificity of unknown effective components in ZNC, ensuring a similarity of more than 90% and a quantitative accuracy of greater than 99.9% for the products from different batches. Finally, the bioactivity of industrially produced ZNC was evaluated in the field, and it significantly increased the potato yields by 4.4–10.8%. Overall, we have established a practical technical system for the large-scale application of ZNC in agriculture.https://www.frontiersin.org/articles/10.3389/fbioe.2021.671879/fullendophytesmetabolitesPaecilomyces variotii SJ1large-scale applicationagriculture |
| spellingShingle | Qingbin Wang Qingbin Wang Chune Peng Liran Shi Zhiguang Liu Dafa Zhou Hui Meng Hongling Zhao Fuchuan Li Min Zhang A Technical System for the Large-Scale Application of Metabolites From Paecilomyces variotii SJ1 in Agriculture Frontiers in Bioengineering and Biotechnology endophytes metabolites Paecilomyces variotii SJ1 large-scale application agriculture |
| title | A Technical System for the Large-Scale Application of Metabolites From Paecilomyces variotii SJ1 in Agriculture |
| title_full | A Technical System for the Large-Scale Application of Metabolites From Paecilomyces variotii SJ1 in Agriculture |
| title_fullStr | A Technical System for the Large-Scale Application of Metabolites From Paecilomyces variotii SJ1 in Agriculture |
| title_full_unstemmed | A Technical System for the Large-Scale Application of Metabolites From Paecilomyces variotii SJ1 in Agriculture |
| title_short | A Technical System for the Large-Scale Application of Metabolites From Paecilomyces variotii SJ1 in Agriculture |
| title_sort | technical system for the large scale application of metabolites from paecilomyces variotii sj1 in agriculture |
| topic | endophytes metabolites Paecilomyces variotii SJ1 large-scale application agriculture |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2021.671879/full |
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