Identification of regulatory links between transcription and RNA processing with long-read sequencing

Identification of regulatory links between transcription and RNA processing with long-read sequencing

Summary: We present a detailed protocol for sequencing full-length mRNA isoforms using the Oxford nanopore long-read sequencing technology. We describe steps for poly(A) RNA isolation, library preparation, and cDNA size selection. We then detail procedures for sequencing and processing and a computa...

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Bibliographic Details
Main Authors: Carlos Alfonso-Gonzalez, Laura Arrigoni, Hasan Can Ozbulut, Stefanie Falk, Ulrike Bönisch, Valérie Hilgers
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Bioinformatics
Sequence Analysis
Genomics
Sequencing
RNAseq
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723004720

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http://www.sciencedirect.com/science/article/pii/S2666166723004720

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