Intrinsic anti-Stokes emission in living HeLa cells.
Intrinsic fluorescence of biological material, also called auto-fluorescence, is a well-known phenomenon and has in recent years been used for imaging, diagnostics and cell viability studies. Here we show that in addition to commonly observed auto-fluorescence, intrinsic anti-Stokes emission can als...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0230441 |
| _version_ | 1818405166200651776 |
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| author | Laura Kacenauskaite Dovydas Gabrielaitis Nicolai Bærentsen Karen L Martinez Tom Vosch Bo W Laursen |
| author_facet | Laura Kacenauskaite Dovydas Gabrielaitis Nicolai Bærentsen Karen L Martinez Tom Vosch Bo W Laursen |
| author_sort | Laura Kacenauskaite |
| collection | DOAJ |
| description | Intrinsic fluorescence of biological material, also called auto-fluorescence, is a well-known phenomenon and has in recent years been used for imaging, diagnostics and cell viability studies. Here we show that in addition to commonly observed auto-fluorescence, intrinsic anti-Stokes emission can also be observed under 560 nm or 633 nm excitation. The anti-Stokes emission is shown to be spatially located on/in the mitochondria. The findings presented here show that sensitive imaging experiments e.g. single molecule experiments or two-photon excitation imaging can be compromised if intracellular anti-Stokes emission is not accounted for. On the other hand, we suggest that this anti-Stokes emission could be exploited as an additional modality for mitochondria visualization and cell viability investigation even in systems that are already labeled with commonly used fluorophores that rely on normal Stokes-based detection. |
| first_indexed | 2024-12-14T08:51:43Z |
| format | Article |
| id | doaj.art-27c80000440146418bdd0c93aee9e64c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T08:51:43Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-27c80000440146418bdd0c93aee9e64c2022-12-21T23:09:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01153e023044110.1371/journal.pone.0230441Intrinsic anti-Stokes emission in living HeLa cells.Laura KacenauskaiteDovydas GabrielaitisNicolai BærentsenKaren L MartinezTom VoschBo W LaursenIntrinsic fluorescence of biological material, also called auto-fluorescence, is a well-known phenomenon and has in recent years been used for imaging, diagnostics and cell viability studies. Here we show that in addition to commonly observed auto-fluorescence, intrinsic anti-Stokes emission can also be observed under 560 nm or 633 nm excitation. The anti-Stokes emission is shown to be spatially located on/in the mitochondria. The findings presented here show that sensitive imaging experiments e.g. single molecule experiments or two-photon excitation imaging can be compromised if intracellular anti-Stokes emission is not accounted for. On the other hand, we suggest that this anti-Stokes emission could be exploited as an additional modality for mitochondria visualization and cell viability investigation even in systems that are already labeled with commonly used fluorophores that rely on normal Stokes-based detection.https://doi.org/10.1371/journal.pone.0230441 |
| spellingShingle | Laura Kacenauskaite Dovydas Gabrielaitis Nicolai Bærentsen Karen L Martinez Tom Vosch Bo W Laursen Intrinsic anti-Stokes emission in living HeLa cells. PLoS ONE |
| title | Intrinsic anti-Stokes emission in living HeLa cells. |
| title_full | Intrinsic anti-Stokes emission in living HeLa cells. |
| title_fullStr | Intrinsic anti-Stokes emission in living HeLa cells. |
| title_full_unstemmed | Intrinsic anti-Stokes emission in living HeLa cells. |
| title_short | Intrinsic anti-Stokes emission in living HeLa cells. |
| title_sort | intrinsic anti stokes emission in living hela cells |
| url | https://doi.org/10.1371/journal.pone.0230441 |
| work_keys_str_mv | AT laurakacenauskaite intrinsicantistokesemissioninlivinghelacells AT dovydasgabrielaitis intrinsicantistokesemissioninlivinghelacells AT nicolaibærentsen intrinsicantistokesemissioninlivinghelacells AT karenlmartinez intrinsicantistokesemissioninlivinghelacells AT tomvosch intrinsicantistokesemissioninlivinghelacells AT bowlaursen intrinsicantistokesemissioninlivinghelacells |