Pilot study on cultural and metagenomic analysis of bile and biliary stentslead to unveiling the key players in stent occlusion
Abstract Endoscopic Retrograde Cholangio-Pancreatography (ERCP) with biliary stenting is a minimally invasive medical procedure employed to address both malignant and benign obstructions within the biliary tract. Benign biliary strictures (BBSs), typically arising from surgical interventions such as...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-51480-2 |
| _version_ | 1827327694241529856 |
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| author | Margherita Cacaci Flavio De Maio Maria Valeria Matteo Brunella Posteraro Maura Di Vito Giulia Menchinelli Andrea Tringali Francesca Romana Monzo Riccardo Torelli Guido Costamagna Cristiano Spada Francesca Bugli Maurizio Sanguinetti Ivo Boskoski |
| author_facet | Margherita Cacaci Flavio De Maio Maria Valeria Matteo Brunella Posteraro Maura Di Vito Giulia Menchinelli Andrea Tringali Francesca Romana Monzo Riccardo Torelli Guido Costamagna Cristiano Spada Francesca Bugli Maurizio Sanguinetti Ivo Boskoski |
| author_sort | Margherita Cacaci |
| collection | DOAJ |
| description | Abstract Endoscopic Retrograde Cholangio-Pancreatography (ERCP) with biliary stenting is a minimally invasive medical procedure employed to address both malignant and benign obstructions within the biliary tract. Benign biliary strictures (BBSs), typically arising from surgical interventions such as liver transplants and cholecystectomy, as well as chronic inflammatory conditions, present a common clinical challenge. The current gold standard for treating BBSs involves the periodic insertion of plastic stents at intervals of 3–4 months, spanning a course of approximately one year. Unfortunately, stent occlusion emerges as a prevalent issue within this treatment paradigm, leading to the recurrence of symptoms and necessitating repeated ERCPs. In response to this clinical concern, we initiated a pilot study, delving into the microbial composition present in bile and on the inner surfaces of plastic stents. This investigation encompassed 22 patients afflicted by BBSs who had previously undergone ERCP with plastic stent placement. Our preliminary findings offered promising insights into the microbial culprits behind stent occlusion, with Enterobacter and Lactobacillus spp. standing out as prominent bacterial species known for their biofilm-forming tendencies on stent surfaces. These revelations hold promise for potential interventions, including targeted antimicrobial therapies aimed at curtailing bacterial growth on stents and the development of advanced stent materials boasting anti-biofilm properties. |
| first_indexed | 2024-03-07T15:05:02Z |
| format | Article |
| id | doaj.art-27c8cb72b2b140dba45b9504351d7a98 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:05:02Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-27c8cb72b2b140dba45b9504351d7a982024-03-05T18:55:02ZengNature PortfolioScientific Reports2045-23222024-02-0114111210.1038/s41598-024-51480-2Pilot study on cultural and metagenomic analysis of bile and biliary stentslead to unveiling the key players in stent occlusionMargherita Cacaci0Flavio De Maio1Maria Valeria Matteo2Brunella Posteraro3Maura Di Vito4Giulia Menchinelli5Andrea Tringali6Francesca Romana Monzo7Riccardo Torelli8Guido Costamagna9Cristiano Spada10Francesca Bugli11Maurizio Sanguinetti12Ivo Boskoski13Department of Basic Biotechnological Sciences, Intensive and Perioperative Clinics, Università Cattolica del Sacro CuoreDepartment of Laboratory and Infectious Sciences, Fondazione Policlinico Universitario A. Gemelli IRCCSDigestive Endoscopy Unit, Fondazione Policlinico Universitario Agostino Gemelli IRCCSDepartment of Basic Biotechnological Sciences, Intensive and Perioperative Clinics, Università Cattolica del Sacro CuoreDepartment of Basic Biotechnological Sciences, Intensive and Perioperative Clinics, Università Cattolica del Sacro CuoreDepartment of Laboratory and Infectious Sciences, Fondazione Policlinico Universitario A. Gemelli IRCCSDigestive Endoscopy Unit, Fondazione Policlinico Universitario Agostino Gemelli IRCCSDepartment of Laboratory and Infectious Sciences, Fondazione Policlinico Universitario A. Gemelli IRCCSDepartment of Laboratory and Infectious Sciences, Fondazione Policlinico Universitario A. Gemelli IRCCSDigestive Endoscopy Unit, Fondazione Policlinico Universitario Agostino Gemelli IRCCSDigestive Endoscopy Unit, Fondazione Policlinico Universitario Agostino Gemelli IRCCSDepartment of Basic Biotechnological Sciences, Intensive and Perioperative Clinics, Università Cattolica del Sacro CuoreDepartment of Basic Biotechnological Sciences, Intensive and Perioperative Clinics, Università Cattolica del Sacro CuoreDigestive Endoscopy Unit, Fondazione Policlinico Universitario Agostino Gemelli IRCCSAbstract Endoscopic Retrograde Cholangio-Pancreatography (ERCP) with biliary stenting is a minimally invasive medical procedure employed to address both malignant and benign obstructions within the biliary tract. Benign biliary strictures (BBSs), typically arising from surgical interventions such as liver transplants and cholecystectomy, as well as chronic inflammatory conditions, present a common clinical challenge. The current gold standard for treating BBSs involves the periodic insertion of plastic stents at intervals of 3–4 months, spanning a course of approximately one year. Unfortunately, stent occlusion emerges as a prevalent issue within this treatment paradigm, leading to the recurrence of symptoms and necessitating repeated ERCPs. In response to this clinical concern, we initiated a pilot study, delving into the microbial composition present in bile and on the inner surfaces of plastic stents. This investigation encompassed 22 patients afflicted by BBSs who had previously undergone ERCP with plastic stent placement. Our preliminary findings offered promising insights into the microbial culprits behind stent occlusion, with Enterobacter and Lactobacillus spp. standing out as prominent bacterial species known for their biofilm-forming tendencies on stent surfaces. These revelations hold promise for potential interventions, including targeted antimicrobial therapies aimed at curtailing bacterial growth on stents and the development of advanced stent materials boasting anti-biofilm properties.https://doi.org/10.1038/s41598-024-51480-2 |
| spellingShingle | Margherita Cacaci Flavio De Maio Maria Valeria Matteo Brunella Posteraro Maura Di Vito Giulia Menchinelli Andrea Tringali Francesca Romana Monzo Riccardo Torelli Guido Costamagna Cristiano Spada Francesca Bugli Maurizio Sanguinetti Ivo Boskoski Pilot study on cultural and metagenomic analysis of bile and biliary stentslead to unveiling the key players in stent occlusion Scientific Reports |
| title | Pilot study on cultural and metagenomic analysis of bile and biliary stentslead to unveiling the key players in stent occlusion |
| title_full | Pilot study on cultural and metagenomic analysis of bile and biliary stentslead to unveiling the key players in stent occlusion |
| title_fullStr | Pilot study on cultural and metagenomic analysis of bile and biliary stentslead to unveiling the key players in stent occlusion |
| title_full_unstemmed | Pilot study on cultural and metagenomic analysis of bile and biliary stentslead to unveiling the key players in stent occlusion |
| title_short | Pilot study on cultural and metagenomic analysis of bile and biliary stentslead to unveiling the key players in stent occlusion |
| title_sort | pilot study on cultural and metagenomic analysis of bile and biliary stentslead to unveiling the key players in stent occlusion |
| url | https://doi.org/10.1038/s41598-024-51480-2 |
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