Porphyrin-Based Covalent Organic Frameworks with Donor-Acceptor Structure for Enhanced Peroxidase-like Activity as a Colorimetric Biosensing Platform
Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and glucose play a key role in many cellular signaling pathways. The efficient and accurate in situ detection of H<sub>2</sub>O<sub>2</sub> released from living cells has attracted extensive research inter...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/2/188 |
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| author | Qian Wang Liang Lv Wenhao Chi Yujiao Bai Wenqing Gao Peihua Zhu Jinghua Yu |
| author_facet | Qian Wang Liang Lv Wenhao Chi Yujiao Bai Wenqing Gao Peihua Zhu Jinghua Yu |
| author_sort | Qian Wang |
| collection | DOAJ |
| description | Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and glucose play a key role in many cellular signaling pathways. The efficient and accurate in situ detection of H<sub>2</sub>O<sub>2</sub> released from living cells has attracted extensive research interests. Herein, a new porphyrin-based porous covalent organic framework (TAP-COF) was fabricated via one-step condensation of 1,6,7,12-tetrachloroperylene tetracarboxylic acid dianhydride and 5,10,15,20-tetrakis (4-aminophenyl)porphyrin iron(III). The obtained TAP-COF has high surface areas, abundant surface catalytic active sites, and highly effective electron transport due to its precisely controllable donor–acceptor arrangement and 3D porous structure. Then, the new TAP-COF exhibited excellent peroxidase-like catalytic activity, which could effectively catalyze oxidation of the substrate 3,3′,5,5′-tetramethylbenzidine by H<sub>2</sub>O<sub>2</sub> to produce a typical blue-colored reaction. On this basis, simple, rapid and selective colorimetric methods for in situ H<sub>2</sub>O<sub>2</sub> detection were developed with the detection limit of 2.6 nM in the wide range of 0.01 to 200 μM. The colorimetric approach also could be used for in situ detection of H<sub>2</sub>O<sub>2</sub> released from living MCF-7 cells. This portable sensor based on a COF nanozyme not only opens a new path for point-of-care testing, but also has potential applications in the field of cell biology and clinical diagnosis. |
| first_indexed | 2024-03-11T09:04:45Z |
| format | Article |
| id | doaj.art-27ca4ab57d4a429bb9f4596bfd6d7877 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T09:04:45Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
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| series | Biosensors |
| spelling | doaj.art-27ca4ab57d4a429bb9f4596bfd6d78772023-11-16T19:25:17ZengMDPI AGBiosensors2079-63742023-01-0113218810.3390/bios13020188Porphyrin-Based Covalent Organic Frameworks with Donor-Acceptor Structure for Enhanced Peroxidase-like Activity as a Colorimetric Biosensing PlatformQian Wang0Liang Lv1Wenhao Chi2Yujiao Bai3Wenqing Gao4Peihua Zhu5Jinghua Yu6School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, ChinaJinan Agricultural Product Quality and Safety Center, Jinan 250316, ChinaSchool of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, ChinaSchool of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, ChinaSchool of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, ChinaSchool of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, ChinaSchool of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, ChinaHydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and glucose play a key role in many cellular signaling pathways. The efficient and accurate in situ detection of H<sub>2</sub>O<sub>2</sub> released from living cells has attracted extensive research interests. Herein, a new porphyrin-based porous covalent organic framework (TAP-COF) was fabricated via one-step condensation of 1,6,7,12-tetrachloroperylene tetracarboxylic acid dianhydride and 5,10,15,20-tetrakis (4-aminophenyl)porphyrin iron(III). The obtained TAP-COF has high surface areas, abundant surface catalytic active sites, and highly effective electron transport due to its precisely controllable donor–acceptor arrangement and 3D porous structure. Then, the new TAP-COF exhibited excellent peroxidase-like catalytic activity, which could effectively catalyze oxidation of the substrate 3,3′,5,5′-tetramethylbenzidine by H<sub>2</sub>O<sub>2</sub> to produce a typical blue-colored reaction. On this basis, simple, rapid and selective colorimetric methods for in situ H<sub>2</sub>O<sub>2</sub> detection were developed with the detection limit of 2.6 nM in the wide range of 0.01 to 200 μM. The colorimetric approach also could be used for in situ detection of H<sub>2</sub>O<sub>2</sub> released from living MCF-7 cells. This portable sensor based on a COF nanozyme not only opens a new path for point-of-care testing, but also has potential applications in the field of cell biology and clinical diagnosis.https://www.mdpi.com/2079-6374/13/2/188porphyrincovalent organic frameworkperoxidase-like activitycellsH<sub>2</sub>O<sub>2</sub> |
| spellingShingle | Qian Wang Liang Lv Wenhao Chi Yujiao Bai Wenqing Gao Peihua Zhu Jinghua Yu Porphyrin-Based Covalent Organic Frameworks with Donor-Acceptor Structure for Enhanced Peroxidase-like Activity as a Colorimetric Biosensing Platform Biosensors porphyrin covalent organic framework peroxidase-like activity cells H<sub>2</sub>O<sub>2</sub> |
| title | Porphyrin-Based Covalent Organic Frameworks with Donor-Acceptor Structure for Enhanced Peroxidase-like Activity as a Colorimetric Biosensing Platform |
| title_full | Porphyrin-Based Covalent Organic Frameworks with Donor-Acceptor Structure for Enhanced Peroxidase-like Activity as a Colorimetric Biosensing Platform |
| title_fullStr | Porphyrin-Based Covalent Organic Frameworks with Donor-Acceptor Structure for Enhanced Peroxidase-like Activity as a Colorimetric Biosensing Platform |
| title_full_unstemmed | Porphyrin-Based Covalent Organic Frameworks with Donor-Acceptor Structure for Enhanced Peroxidase-like Activity as a Colorimetric Biosensing Platform |
| title_short | Porphyrin-Based Covalent Organic Frameworks with Donor-Acceptor Structure for Enhanced Peroxidase-like Activity as a Colorimetric Biosensing Platform |
| title_sort | porphyrin based covalent organic frameworks with donor acceptor structure for enhanced peroxidase like activity as a colorimetric biosensing platform |
| topic | porphyrin covalent organic framework peroxidase-like activity cells H<sub>2</sub>O<sub>2</sub> |
| url | https://www.mdpi.com/2079-6374/13/2/188 |
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