The effects of ethylene on the HCl-extractability of trace elements during soybean seed germination
Background: Ethylene is capable of promoting seed germination in some plant species. Mobilization of metals such as Fe, Cu, Mn, and Zn in mature seeds takes place when seeds are germinating. However, whether ethylene is involved in the regulation of soybean seed germination and metal element mobiliz...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2015-09-01
|
| Series: | Electronic Journal of Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345815000858 |
| _version_ | 1817977224207269888 |
|---|---|
| author | Chunming Liu Yixin Zhao Jianfeng Liu Wanting Gen Yunqing Cheng |
| author_facet | Chunming Liu Yixin Zhao Jianfeng Liu Wanting Gen Yunqing Cheng |
| author_sort | Chunming Liu |
| collection | DOAJ |
| description | Background: Ethylene is capable of promoting seed germination in some plant species. Mobilization of metals such as Fe, Cu, Mn, and Zn in mature seeds takes place when seeds are germinating. However, whether ethylene is involved in the regulation of soybean seed germination and metal element mobilization during early seed germination stage remains unknown. In the present study, seeds were treated with ethylene synthesis inhibitor aminoethoxyvinylglycine (AVG) and ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), and double distilled H2O (ddH20) treatment was used as control. Ethylene emission, ACC synthase (ACS) expression, ACS enzyme activity and Ca, Zn, Mn, Cu and Fe content in hypocotyls were qualified to analyze the relationship between ethylene and mobilization of these elements.
Results: The results showed that ACS expression, ACS enzyme activity and ethylene emission peaked at 1 and 7 d after sowing. AVG inhibited ethylene production, promoted the hypocotyls length, ACS expression and its activity, concentrations of total and HCl-extractable Zn, and HCl-extractable Fe in hypocotyls, while ACC caused opposite effects. AVG and ACC treatment had no significantly effects on total and HCl-extractable Ca, Cu and HCl-extractable Mn. Total Mn concentration was promoted by AVG at 1, 3, and 5 d significantly, while ACC treatment tended to have no significantly effects on Mn concentration.
Conclusion: These findings suggested that ethylene is at least partly involved in the regulation of soybean seed germination. Remobilization of Zn and Fe may be negatively regulated by ethylene. |
| first_indexed | 2024-04-13T22:14:00Z |
| format | Article |
| id | doaj.art-27d0787901c844439c34fdedb0a54e9b |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-04-13T22:14:00Z |
| publishDate | 2015-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-27d0787901c844439c34fdedb0a54e9b2022-12-22T02:27:37ZengElsevierElectronic Journal of Biotechnology0717-34582015-09-0118533333710.1016/j.ejbt.2015.06.003The effects of ethylene on the HCl-extractability of trace elements during soybean seed germinationChunming LiuYixin ZhaoJianfeng LiuWanting GenYunqing ChengBackground: Ethylene is capable of promoting seed germination in some plant species. Mobilization of metals such as Fe, Cu, Mn, and Zn in mature seeds takes place when seeds are germinating. However, whether ethylene is involved in the regulation of soybean seed germination and metal element mobilization during early seed germination stage remains unknown. In the present study, seeds were treated with ethylene synthesis inhibitor aminoethoxyvinylglycine (AVG) and ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), and double distilled H2O (ddH20) treatment was used as control. Ethylene emission, ACC synthase (ACS) expression, ACS enzyme activity and Ca, Zn, Mn, Cu and Fe content in hypocotyls were qualified to analyze the relationship between ethylene and mobilization of these elements. Results: The results showed that ACS expression, ACS enzyme activity and ethylene emission peaked at 1 and 7 d after sowing. AVG inhibited ethylene production, promoted the hypocotyls length, ACS expression and its activity, concentrations of total and HCl-extractable Zn, and HCl-extractable Fe in hypocotyls, while ACC caused opposite effects. AVG and ACC treatment had no significantly effects on total and HCl-extractable Ca, Cu and HCl-extractable Mn. Total Mn concentration was promoted by AVG at 1, 3, and 5 d significantly, while ACC treatment tended to have no significantly effects on Mn concentration. Conclusion: These findings suggested that ethylene is at least partly involved in the regulation of soybean seed germination. Remobilization of Zn and Fe may be negatively regulated by ethylene.http://www.sciencedirect.com/science/article/pii/S0717345815000858ACC synthaseBioavailabilityEthyleneMetallic elementsSeed germinationSoybean |
| spellingShingle | Chunming Liu Yixin Zhao Jianfeng Liu Wanting Gen Yunqing Cheng The effects of ethylene on the HCl-extractability of trace elements during soybean seed germination Electronic Journal of Biotechnology ACC synthase Bioavailability Ethylene Metallic elements Seed germination Soybean |
| title | The effects of ethylene on the HCl-extractability of trace elements during soybean seed germination |
| title_full | The effects of ethylene on the HCl-extractability of trace elements during soybean seed germination |
| title_fullStr | The effects of ethylene on the HCl-extractability of trace elements during soybean seed germination |
| title_full_unstemmed | The effects of ethylene on the HCl-extractability of trace elements during soybean seed germination |
| title_short | The effects of ethylene on the HCl-extractability of trace elements during soybean seed germination |
| title_sort | effects of ethylene on the hcl extractability of trace elements during soybean seed germination |
| topic | ACC synthase Bioavailability Ethylene Metallic elements Seed germination Soybean |
| url | http://www.sciencedirect.com/science/article/pii/S0717345815000858 |
| work_keys_str_mv | AT chunmingliu theeffectsofethyleneonthehclextractabilityoftraceelementsduringsoybeanseedgermination AT yixinzhao theeffectsofethyleneonthehclextractabilityoftraceelementsduringsoybeanseedgermination AT jianfengliu theeffectsofethyleneonthehclextractabilityoftraceelementsduringsoybeanseedgermination AT wantinggen theeffectsofethyleneonthehclextractabilityoftraceelementsduringsoybeanseedgermination AT yunqingcheng theeffectsofethyleneonthehclextractabilityoftraceelementsduringsoybeanseedgermination AT chunmingliu effectsofethyleneonthehclextractabilityoftraceelementsduringsoybeanseedgermination AT yixinzhao effectsofethyleneonthehclextractabilityoftraceelementsduringsoybeanseedgermination AT jianfengliu effectsofethyleneonthehclextractabilityoftraceelementsduringsoybeanseedgermination AT wantinggen effectsofethyleneonthehclextractabilityoftraceelementsduringsoybeanseedgermination AT yunqingcheng effectsofethyleneonthehclextractabilityoftraceelementsduringsoybeanseedgermination |