Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil
Objective: The present study was undertaken to characterize CTX-M ESBL-producing Klebsiella pneumoniae collected from hospitals in different cities of Brazil. Material and Methods: Eighty-five K. pneumoniae strains isolated from hospitalized patients in six different hospitals of three cities of Bra...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2011-09-01
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| Series: | Brazilian Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1413867011702227 |
| _version_ | 1818293561851904000 |
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| author | Thiago Pavoni Gomes Chagas Ronaldo Mendes Alves Deyse Christina Vallim, PhD Liliane Miyuki Seki, MSc Leila Carvalho Campos, PhD Marise Dutra Asensi, PhD |
| author_facet | Thiago Pavoni Gomes Chagas Ronaldo Mendes Alves Deyse Christina Vallim, PhD Liliane Miyuki Seki, MSc Leila Carvalho Campos, PhD Marise Dutra Asensi, PhD |
| author_sort | Thiago Pavoni Gomes Chagas |
| collection | DOAJ |
| description | Objective: The present study was undertaken to characterize CTX-M ESBL-producing Klebsiella pneumoniae collected from hospitals in different cities of Brazil. Material and Methods: Eighty-five K. pneumoniae strains isolated from hospitalized patients in six different hospitals of three cities of Brazil were analyzed. ESBL production was confirmed by the standard double-disk synergy test and the Etest®. The MIC50 and MIC90 for ESBL-producing isolates were determined by the Etest® method. The antimicrobial susceptibilities of bacterial isolates were determined using the agar diffusion method according to the CLSI. Screening for blaTEM, blaSHV, blaCTX-M genes and class 1 integron was performed by PCR amplification. To determine the genomic diversity of CTX-M-producers, isolates were analyzed by macrorestriction profile analysis following PFGE. Results and Discussion: Seventy-one K. pneumoniae isolates were ESBL-producing. PCR and sequencing experiments detected 38 CTX-M-producing K. pneumoniae belonged to groups CTX-M 1, CTX-M 2, CTX-M 8 and CTX-M 9. The association of different types ESBL (CTX-M, SHV and TEM) was frequent. All K. pneumoniae isolates carried class 1 integron. PFGE analysis revealed thirty-one clonal types among CTX-M-producing isolates. The data presented herein illustrate the diversity of genotypes of CTX-M producing K. pneumoniae among Brazilians hospitals. Keywords: Klebsiella pneumoniae, β-lactamases, genotype |
| first_indexed | 2024-12-13T03:17:49Z |
| format | Article |
| id | doaj.art-27d211c29f33472d92846bfccd8bfb3d |
| institution | Directory Open Access Journal |
| issn | 1413-8670 |
| language | English |
| last_indexed | 2024-12-13T03:17:49Z |
| publishDate | 2011-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-27d211c29f33472d92846bfccd8bfb3d2022-12-22T00:01:25ZengElsevierBrazilian Journal of Infectious Diseases1413-86702011-09-01155420425Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in BrazilThiago Pavoni Gomes Chagas0Ronaldo Mendes Alves1Deyse Christina Vallim, PhD2Liliane Miyuki Seki, MSc3Leila Carvalho Campos, PhD4Marise Dutra Asensi, PhD5Graduated in Biological Sciences, Universidade do Estado do Rio de Janeiro (UERJ); MSc Student in Tropical Medicine, Instituto Oswaldo Cruz (IOC)/Fiocruz, Rio de Janeiro, RJ, BrazilGraduated in Biological Sciences; Technologist, IOC/FIOCRUZ, Rio de Janeiro, RJ, BrazilMicrobiology, Universidade Federal do Rio de Janeiro (UFRJ); Technologist, IOC/Fiocruz, Rio de Janeiro, RJ, BrazilMicrobiology, Universidade Federal Rural do Rio de Janeiro (UFRRJ); Technician, IOC/Fiocruz, Rio de Janeiro, RJ, BrazilMicrobiology, Researcher, CPqGM/Fiocruz, Rio de Janeiro, RJ, BrazilMicrobiology; Chief, Hospital Infection Research Laboratory, IOC/Fiocruz, Rio de Janeiro, RJ, Brazil; Correspondence to: Av. Brasil, 4365, Manguinhos Rio de Janeiro - RJ - Brazil CEP: 21040-360Objective: The present study was undertaken to characterize CTX-M ESBL-producing Klebsiella pneumoniae collected from hospitals in different cities of Brazil. Material and Methods: Eighty-five K. pneumoniae strains isolated from hospitalized patients in six different hospitals of three cities of Brazil were analyzed. ESBL production was confirmed by the standard double-disk synergy test and the Etest®. The MIC50 and MIC90 for ESBL-producing isolates were determined by the Etest® method. The antimicrobial susceptibilities of bacterial isolates were determined using the agar diffusion method according to the CLSI. Screening for blaTEM, blaSHV, blaCTX-M genes and class 1 integron was performed by PCR amplification. To determine the genomic diversity of CTX-M-producers, isolates were analyzed by macrorestriction profile analysis following PFGE. Results and Discussion: Seventy-one K. pneumoniae isolates were ESBL-producing. PCR and sequencing experiments detected 38 CTX-M-producing K. pneumoniae belonged to groups CTX-M 1, CTX-M 2, CTX-M 8 and CTX-M 9. The association of different types ESBL (CTX-M, SHV and TEM) was frequent. All K. pneumoniae isolates carried class 1 integron. PFGE analysis revealed thirty-one clonal types among CTX-M-producing isolates. The data presented herein illustrate the diversity of genotypes of CTX-M producing K. pneumoniae among Brazilians hospitals. Keywords: Klebsiella pneumoniae, β-lactamases, genotypehttp://www.sciencedirect.com/science/article/pii/S1413867011702227 |
| spellingShingle | Thiago Pavoni Gomes Chagas Ronaldo Mendes Alves Deyse Christina Vallim, PhD Liliane Miyuki Seki, MSc Leila Carvalho Campos, PhD Marise Dutra Asensi, PhD Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil Brazilian Journal of Infectious Diseases |
| title | Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil |
| title_full | Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil |
| title_fullStr | Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil |
| title_full_unstemmed | Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil |
| title_short | Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil |
| title_sort | diversity of genotypes in ctx m producing klebsiella pneumoniae isolated in different hospitals in brazil |
| url | http://www.sciencedirect.com/science/article/pii/S1413867011702227 |
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