Protective effects of hydroalcoholic extract of Stachys pilifera on paracetamol-induced nephrotoxicity in female rats
Background and purpose: Stachys pilifera is used in traditional medicine due to its antioxidant, anti-inflammatory, and antimicrobial effects. The goal of this study was to examine the renoprotective activity of the hydroalcoholic extract of aerial parts of S. pilifera on paracetamol (PCM)-induced n...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2021-01-01
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| Series: | Research in Pharmaceutical Sciences |
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| Online Access: | http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2021;volume=16;issue=6;spage=643;epage=650;aulast=Rabani |
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| author | Mohammad Reza Rabani Nahid Azarmehr Zahra Moslemi Heibatollah Sadeghi Hossein Amini-Khoei Amir Hossein Doustimotlagh |
| author_facet | Mohammad Reza Rabani Nahid Azarmehr Zahra Moslemi Heibatollah Sadeghi Hossein Amini-Khoei Amir Hossein Doustimotlagh |
| author_sort | Mohammad Reza Rabani |
| collection | DOAJ |
| description | Background and purpose: Stachys pilifera is used in traditional medicine due to its antioxidant, anti-inflammatory, and antimicrobial effects. The goal of this study was to examine the renoprotective activity of the hydroalcoholic extract of aerial parts of S. pilifera on paracetamol (PCM)-induced nephrotoxicity.
Experimental approach: The Wistar female rats were randomly divided into four groups including control, PCM, S. pilifera hydroalcoholic extract (SPE), and PCM + SPE. The animals received SPE (500 mg/kg) for one week and PCM (3 g/kg) on the 6th day orally. Kidney function tests and oxidant/antioxidant markers were determined in serum and tissue homogenate, respectively. Protein and mRNA levels of TNF-α, as well as hematoxylin and eosin staining, were assessed in the kidney tissue.
Findings/Results: Treatment with SPE in the PCM group significantly decreased blood urea nitrogen and creatinine against the merely PCM rats (P < 0.05). The amount of nitric oxide metabolite and superoxide dismutase activity in the group receiving SPE showed a significant increase compared to PCM rats (P < 0.05). A significant difference in TNF-α levels between the groups was not observed. Histological changes were improved in the rats treated with SPE.
Conclusion and implications: Totally, our findings showed that SPE can inhibit PCM nephrotoxicity by enhancing kidney function markers, antioxidant status, and histological changes. Though, more researches are required to estimate the possible mechanism of SPE. |
| first_indexed | 2024-12-17T12:45:34Z |
| format | Article |
| id | doaj.art-27d29dff96f94c2cbd04d3b95221b977 |
| institution | Directory Open Access Journal |
| issn | 1735-5362 1735-9414 |
| language | English |
| last_indexed | 2024-12-17T12:45:34Z |
| publishDate | 2021-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Research in Pharmaceutical Sciences |
| spelling | doaj.art-27d29dff96f94c2cbd04d3b95221b9772022-12-21T21:47:45ZengWolters Kluwer Medknow PublicationsResearch in Pharmaceutical Sciences1735-53621735-94142021-01-0116664365010.4103/1735-5362.327510Protective effects of hydroalcoholic extract of Stachys pilifera on paracetamol-induced nephrotoxicity in female ratsMohammad Reza RabaniNahid AzarmehrZahra MoslemiHeibatollah SadeghiHossein Amini-KhoeiAmir Hossein DoustimotlaghBackground and purpose: Stachys pilifera is used in traditional medicine due to its antioxidant, anti-inflammatory, and antimicrobial effects. The goal of this study was to examine the renoprotective activity of the hydroalcoholic extract of aerial parts of S. pilifera on paracetamol (PCM)-induced nephrotoxicity. Experimental approach: The Wistar female rats were randomly divided into four groups including control, PCM, S. pilifera hydroalcoholic extract (SPE), and PCM + SPE. The animals received SPE (500 mg/kg) for one week and PCM (3 g/kg) on the 6th day orally. Kidney function tests and oxidant/antioxidant markers were determined in serum and tissue homogenate, respectively. Protein and mRNA levels of TNF-α, as well as hematoxylin and eosin staining, were assessed in the kidney tissue. Findings/Results: Treatment with SPE in the PCM group significantly decreased blood urea nitrogen and creatinine against the merely PCM rats (P < 0.05). The amount of nitric oxide metabolite and superoxide dismutase activity in the group receiving SPE showed a significant increase compared to PCM rats (P < 0.05). A significant difference in TNF-α levels between the groups was not observed. Histological changes were improved in the rats treated with SPE. Conclusion and implications: Totally, our findings showed that SPE can inhibit PCM nephrotoxicity by enhancing kidney function markers, antioxidant status, and histological changes. Though, more researches are required to estimate the possible mechanism of SPE.http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2021;volume=16;issue=6;spage=643;epage=650;aulast=Rabaniantioxidant; nephrotoxicity; paracetamol; stachys pilifera. |
| spellingShingle | Mohammad Reza Rabani Nahid Azarmehr Zahra Moslemi Heibatollah Sadeghi Hossein Amini-Khoei Amir Hossein Doustimotlagh Protective effects of hydroalcoholic extract of Stachys pilifera on paracetamol-induced nephrotoxicity in female rats Research in Pharmaceutical Sciences antioxidant; nephrotoxicity; paracetamol; stachys pilifera. |
| title | Protective effects of hydroalcoholic extract of Stachys pilifera on paracetamol-induced nephrotoxicity in female rats |
| title_full | Protective effects of hydroalcoholic extract of Stachys pilifera on paracetamol-induced nephrotoxicity in female rats |
| title_fullStr | Protective effects of hydroalcoholic extract of Stachys pilifera on paracetamol-induced nephrotoxicity in female rats |
| title_full_unstemmed | Protective effects of hydroalcoholic extract of Stachys pilifera on paracetamol-induced nephrotoxicity in female rats |
| title_short | Protective effects of hydroalcoholic extract of Stachys pilifera on paracetamol-induced nephrotoxicity in female rats |
| title_sort | protective effects of hydroalcoholic extract of stachys pilifera on paracetamol induced nephrotoxicity in female rats |
| topic | antioxidant; nephrotoxicity; paracetamol; stachys pilifera. |
| url | http://www.rpsjournal.net/article.asp?issn=1735-5362;year=2021;volume=16;issue=6;spage=643;epage=650;aulast=Rabani |
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