Optimization of nitrogen source for Bifidobacterium bifidum using response surface methodology
In order to improve the viable counts of Bifidobacterium bifidum BB01 in the liquid medium, the Central Composite Design (CCD) was used to optimize the nitrogen source in the medium of B. bifidum BB01. The results showed that the nitrogen source composition of B. bifidum BB01 was: peptone 0.9%, yeas...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2016-06-01
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| Series: | Acta Universitatis Cibiniensis. Series E: Food Technology |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/aucft-2016-0004 |
| _version_ | 1818908023653924864 |
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| author | Chen He Kou Jianbo Hu Man Shu Guowei |
| author_facet | Chen He Kou Jianbo Hu Man Shu Guowei |
| author_sort | Chen He |
| collection | DOAJ |
| description | In order to improve the viable counts of Bifidobacterium bifidum BB01 in the liquid medium, the Central Composite Design (CCD) was used to optimize the nitrogen source in the medium of B. bifidum BB01. The results showed that the nitrogen source composition of B. bifidum BB01 was: peptone 0.9%, yeast extracts 0.3%, beef paste 0.7%. Under the optimal conditions, the viable counts of B. bifidum BB01 reached (2.49±0.06)×109CFU/mL after cultured at 18h, which was 42.97% higher than MRS (lactose), and 12.85% higher than the optimized MRS medium (carbon source and prebiotics were optimized). Therefore, the CCD used in this study is workable for promoting the growth of B. bifidum BB01. |
| first_indexed | 2024-12-19T22:04:26Z |
| format | Article |
| id | doaj.art-27d50a8a05d54b37bc4a3de48178fbda |
| institution | Directory Open Access Journal |
| issn | 2344-150X |
| language | English |
| last_indexed | 2024-12-19T22:04:26Z |
| publishDate | 2016-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Universitatis Cibiniensis. Series E: Food Technology |
| spelling | doaj.art-27d50a8a05d54b37bc4a3de48178fbda2022-12-21T20:04:03ZengSciendoActa Universitatis Cibiniensis. Series E: Food Technology2344-150X2016-06-01201536410.1515/aucft-2016-0004aucft-2016-0004Optimization of nitrogen source for Bifidobacterium bifidum using response surface methodologyChen He0Kou Jianbo1Hu Man2Shu Guowei3School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi’ an, ChinaSchool of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi’ an, ChinaSchool of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi’ an, ChinaSchool of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi’ an, ChinaIn order to improve the viable counts of Bifidobacterium bifidum BB01 in the liquid medium, the Central Composite Design (CCD) was used to optimize the nitrogen source in the medium of B. bifidum BB01. The results showed that the nitrogen source composition of B. bifidum BB01 was: peptone 0.9%, yeast extracts 0.3%, beef paste 0.7%. Under the optimal conditions, the viable counts of B. bifidum BB01 reached (2.49±0.06)×109CFU/mL after cultured at 18h, which was 42.97% higher than MRS (lactose), and 12.85% higher than the optimized MRS medium (carbon source and prebiotics were optimized). Therefore, the CCD used in this study is workable for promoting the growth of B. bifidum BB01.https://doi.org/10.1515/aucft-2016-0004bifidobacterium bifidumresponse surface methodologyoptimizationnitrogen source |
| spellingShingle | Chen He Kou Jianbo Hu Man Shu Guowei Optimization of nitrogen source for Bifidobacterium bifidum using response surface methodology Acta Universitatis Cibiniensis. Series E: Food Technology bifidobacterium bifidum response surface methodology optimization nitrogen source |
| title | Optimization of nitrogen source for Bifidobacterium bifidum using response surface methodology |
| title_full | Optimization of nitrogen source for Bifidobacterium bifidum using response surface methodology |
| title_fullStr | Optimization of nitrogen source for Bifidobacterium bifidum using response surface methodology |
| title_full_unstemmed | Optimization of nitrogen source for Bifidobacterium bifidum using response surface methodology |
| title_short | Optimization of nitrogen source for Bifidobacterium bifidum using response surface methodology |
| title_sort | optimization of nitrogen source for bifidobacterium bifidum using response surface methodology |
| topic | bifidobacterium bifidum response surface methodology optimization nitrogen source |
| url | https://doi.org/10.1515/aucft-2016-0004 |
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