Regular exercise and branched‐chain amino acids prevent ischemic acute kidney injury‐related muscle wasting in mice
Abstract Acute kidney injury (AKI) causes glucose and protein metabolism abnormalities that result in muscle wasting, thereby affecting the long‐term prognosis of critical illness survivors. Here, we examined whether early intervention with treadmill exercise and branched‐chain amino acids (BCAA) ca...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-08-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.14557 |
| _version_ | 1818504054961078272 |
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| author | Soichiro Nagata Akihiko Kato Shinsuke Isobe Tomoyuki Fujikura Naro Ohashi Hiroaki Miyajima Hideo Yasuda |
| author_facet | Soichiro Nagata Akihiko Kato Shinsuke Isobe Tomoyuki Fujikura Naro Ohashi Hiroaki Miyajima Hideo Yasuda |
| author_sort | Soichiro Nagata |
| collection | DOAJ |
| description | Abstract Acute kidney injury (AKI) causes glucose and protein metabolism abnormalities that result in muscle wasting, thereby affecting the long‐term prognosis of critical illness survivors. Here, we examined whether early intervention with treadmill exercise and branched‐chain amino acids (BCAA) can prevent AKI‐related muscle wasting and reduced physical performance in mice. Unilateral 15 min ischemia‐reperfusion injury was induced in contralateral nephrectomized mice, and muscle histological and physiological changes were assessed and compared with those of pair‐fed control mice, since AKI causes severe anorexia. Mice exercised for 30 min each day and received oral BCAA for 7 days after AKI insult. By day 7, ischemic AKI significantly decreased wet weight, myofiber cross‐sectional area, and central mitochondrial volume density of the anterior tibialis muscle, and significantly reduced maximal exercise time. Regular exercise and BCAA prevented AKI‐related muscle wasting and low physical performance by suppressing myostatin and atrogin‐1 mRNA upregulation, and restoring reduced phosphorylated Akt and PGC‐1α mRNA expression in the muscle. Ischemic AKI induces muscle wasting by accelerating muscle protein degradation and reducing protein synthesis; however, we found that regular exercise and BCAA prevented AKI‐related muscle wasting without worsening kidney damage, suggesting that early rehabilitation with nutritional support could prevent AKI‐related muscle wasting. |
| first_indexed | 2024-12-10T21:31:58Z |
| format | Article |
| id | doaj.art-27d72965b508464e8c62152187340fea |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-12-10T21:31:58Z |
| publishDate | 2020-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-27d72965b508464e8c62152187340fea2022-12-22T01:32:46ZengWileyPhysiological Reports2051-817X2020-08-01816n/an/a10.14814/phy2.14557Regular exercise and branched‐chain amino acids prevent ischemic acute kidney injury‐related muscle wasting in miceSoichiro Nagata0Akihiko Kato1Shinsuke Isobe2Tomoyuki Fujikura3Naro Ohashi4Hiroaki Miyajima5Hideo Yasuda6Internal Medicine 1 Hamamatsu University School of Medicine Hamamatsu JapanBlood Purification Unit Hamamatsu University Hospital Hamamatsu JapanInternal Medicine 1 Hamamatsu University School of Medicine Hamamatsu JapanInternal Medicine 1 Hamamatsu University School of Medicine Hamamatsu JapanInternal Medicine 1 Hamamatsu University School of Medicine Hamamatsu JapanInternal Medicine 1 Hamamatsu University School of Medicine Hamamatsu JapanInternal Medicine 1 Hamamatsu University School of Medicine Hamamatsu JapanAbstract Acute kidney injury (AKI) causes glucose and protein metabolism abnormalities that result in muscle wasting, thereby affecting the long‐term prognosis of critical illness survivors. Here, we examined whether early intervention with treadmill exercise and branched‐chain amino acids (BCAA) can prevent AKI‐related muscle wasting and reduced physical performance in mice. Unilateral 15 min ischemia‐reperfusion injury was induced in contralateral nephrectomized mice, and muscle histological and physiological changes were assessed and compared with those of pair‐fed control mice, since AKI causes severe anorexia. Mice exercised for 30 min each day and received oral BCAA for 7 days after AKI insult. By day 7, ischemic AKI significantly decreased wet weight, myofiber cross‐sectional area, and central mitochondrial volume density of the anterior tibialis muscle, and significantly reduced maximal exercise time. Regular exercise and BCAA prevented AKI‐related muscle wasting and low physical performance by suppressing myostatin and atrogin‐1 mRNA upregulation, and restoring reduced phosphorylated Akt and PGC‐1α mRNA expression in the muscle. Ischemic AKI induces muscle wasting by accelerating muscle protein degradation and reducing protein synthesis; however, we found that regular exercise and BCAA prevented AKI‐related muscle wasting without worsening kidney damage, suggesting that early rehabilitation with nutritional support could prevent AKI‐related muscle wasting.https://doi.org/10.14814/phy2.14557acute kidney injurybranched‐chain amino acidexercisemuscle wasting |
| spellingShingle | Soichiro Nagata Akihiko Kato Shinsuke Isobe Tomoyuki Fujikura Naro Ohashi Hiroaki Miyajima Hideo Yasuda Regular exercise and branched‐chain amino acids prevent ischemic acute kidney injury‐related muscle wasting in mice Physiological Reports acute kidney injury branched‐chain amino acid exercise muscle wasting |
| title | Regular exercise and branched‐chain amino acids prevent ischemic acute kidney injury‐related muscle wasting in mice |
| title_full | Regular exercise and branched‐chain amino acids prevent ischemic acute kidney injury‐related muscle wasting in mice |
| title_fullStr | Regular exercise and branched‐chain amino acids prevent ischemic acute kidney injury‐related muscle wasting in mice |
| title_full_unstemmed | Regular exercise and branched‐chain amino acids prevent ischemic acute kidney injury‐related muscle wasting in mice |
| title_short | Regular exercise and branched‐chain amino acids prevent ischemic acute kidney injury‐related muscle wasting in mice |
| title_sort | regular exercise and branched chain amino acids prevent ischemic acute kidney injury related muscle wasting in mice |
| topic | acute kidney injury branched‐chain amino acid exercise muscle wasting |
| url | https://doi.org/10.14814/phy2.14557 |
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