Antibacterial, Antiparasitic, and Cytotoxic Activities of Chemical Characterized Essential Oil of <i>Chrysopogon zizanioides</i> Roots
This study aimed to investigate the chemical composition as well as the antibacterial, antiparasitic, and cytotoxic potentialities of the Brazilian <i>Chrysopogon zizanioides</i> root essential oil (CZ-EO) In addition, CZ-EO cytotoxicity to LLCMK<sub>2</sub> adherent epitheli...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2022-08-01
|
Series: | Pharmaceuticals |
Subjects: | |
Online Access: | https://www.mdpi.com/1424-8247/15/8/967 |
_version_ | 1797431809367080960 |
---|---|
author | Thaís A. S. Oliveira Tatiana M. Vieira Viviane R. Esperandim Carlos H. G. Martins Lizandra G. Magalhães Mayker L. D. Miranda Antônio E. M. Crotti |
author_facet | Thaís A. S. Oliveira Tatiana M. Vieira Viviane R. Esperandim Carlos H. G. Martins Lizandra G. Magalhães Mayker L. D. Miranda Antônio E. M. Crotti |
author_sort | Thaís A. S. Oliveira |
collection | DOAJ |
description | This study aimed to investigate the chemical composition as well as the antibacterial, antiparasitic, and cytotoxic potentialities of the Brazilian <i>Chrysopogon zizanioides</i> root essential oil (CZ-EO) In addition, CZ-EO cytotoxicity to LLCMK<sub>2</sub> adherent epithelial cells was assessed. The major compounds identified in CZ-EO were khusimol (30.0 ± 0.3%), β-eudesmol (10.8 ± 0.3%), α-muurolene (6.0 ± 0.1%), and patchouli alcohol (5.6 ± 0.2%). CZ-EO displayed optimal antibacterial activity against <i>Prevotella nigrescens</i>, <i>Fusobacterium nucleatum</i>, <i>Prevotella melaninogenica</i>, and <i>Aggregatibacter actinomycetemcomitans</i>, with Minimum Inhibitory Concentration (MIC) values between 22 and 62.5 µg/mL and Minimum Bactericidal Concentration (MBC) values between 22 and 400 µg/mL. CZ-EO was highly active against the <i>L. amazonensis</i> promastigote and amastigote forms (IC<sub>50</sub> = 7.20 and 16.21 µg/mL, respectively) and the <i>T. cruzi</i> trypomastigote form (IC<sub>50</sub> = 11.2 µg/mL). Moreover, CZ-EO showed moderate cytotoxicity to LLCMK<sub>2</sub> cells, with CC<sub>50</sub> = 565.4 µg/mL. These results revealed an interesting in vitro selectivity of CZ-EO toward the <i>L. amazonensis</i> promastigote and amastigote forms (Selectivity Index, SI = 78.5 and 34.8, respectively) and the <i>T. cruzi</i> trypomastigote form (SI = 50.5) compared to LLCMK<sub>2</sub> cells. These results showed the promising potential of CZ-EO for developing new antimicrobial, antileishmanial, and antitrypanosomal drugs. |
first_indexed | 2024-03-09T09:50:40Z |
format | Article |
id | doaj.art-27dc20d2a0c44149af34a0a0749fedff |
institution | Directory Open Access Journal |
issn | 1424-8247 |
language | English |
last_indexed | 2024-03-09T09:50:40Z |
publishDate | 2022-08-01 |
publisher | MDPI AG |
record_format | Article |
series | Pharmaceuticals |
spelling | doaj.art-27dc20d2a0c44149af34a0a0749fedff2023-12-02T00:08:56ZengMDPI AGPharmaceuticals1424-82472022-08-0115896710.3390/ph15080967Antibacterial, Antiparasitic, and Cytotoxic Activities of Chemical Characterized Essential Oil of <i>Chrysopogon zizanioides</i> RootsThaís A. S. Oliveira0Tatiana M. Vieira1Viviane R. Esperandim2Carlos H. G. Martins3Lizandra G. Magalhães4Mayker L. D. Miranda5Antônio E. M. Crotti6Department of Chemistry, Faculty of Phylosophy, Sciences, and Letters of Ribeirão Preto, University of São Paulo, Av. Bandeirantes, 3900, Ribeirão Preto 14049-900, SP, BrazilDepartment of Chemistry, Faculty of Phylosophy, Sciences, and Letters of Ribeirão Preto, University of São Paulo, Av. Bandeirantes, 3900, Ribeirão Preto 14049-900, SP, BrazilUniversity of Franca, Av. Dr. Armando Salles Oliveira, 201—Parque Universitário, Franca 14404-600, SP, BrazilInstitute of Biomedical Sciences, Federal University of Uberlândia, Rua Ceará, 1720, Umuarama, Uberlândia 38402-018, MG, BrazilUniversity of Franca, Av. Dr. Armando Salles Oliveira, 201—Parque Universitário, Franca 14404-600, SP, BrazilFederal Institute of Education, Science, and Technology of Triângulo Mineiro, Campus Uberlândia, Centro, Rua Blanche Galassi, 150, Morada da Colina, Uberlândia 38411-104, MG, BrazilDepartment of Chemistry, Faculty of Phylosophy, Sciences, and Letters of Ribeirão Preto, University of São Paulo, Av. Bandeirantes, 3900, Ribeirão Preto 14049-900, SP, BrazilThis study aimed to investigate the chemical composition as well as the antibacterial, antiparasitic, and cytotoxic potentialities of the Brazilian <i>Chrysopogon zizanioides</i> root essential oil (CZ-EO) In addition, CZ-EO cytotoxicity to LLCMK<sub>2</sub> adherent epithelial cells was assessed. The major compounds identified in CZ-EO were khusimol (30.0 ± 0.3%), β-eudesmol (10.8 ± 0.3%), α-muurolene (6.0 ± 0.1%), and patchouli alcohol (5.6 ± 0.2%). CZ-EO displayed optimal antibacterial activity against <i>Prevotella nigrescens</i>, <i>Fusobacterium nucleatum</i>, <i>Prevotella melaninogenica</i>, and <i>Aggregatibacter actinomycetemcomitans</i>, with Minimum Inhibitory Concentration (MIC) values between 22 and 62.5 µg/mL and Minimum Bactericidal Concentration (MBC) values between 22 and 400 µg/mL. CZ-EO was highly active against the <i>L. amazonensis</i> promastigote and amastigote forms (IC<sub>50</sub> = 7.20 and 16.21 µg/mL, respectively) and the <i>T. cruzi</i> trypomastigote form (IC<sub>50</sub> = 11.2 µg/mL). Moreover, CZ-EO showed moderate cytotoxicity to LLCMK<sub>2</sub> cells, with CC<sub>50</sub> = 565.4 µg/mL. These results revealed an interesting in vitro selectivity of CZ-EO toward the <i>L. amazonensis</i> promastigote and amastigote forms (Selectivity Index, SI = 78.5 and 34.8, respectively) and the <i>T. cruzi</i> trypomastigote form (SI = 50.5) compared to LLCMK<sub>2</sub> cells. These results showed the promising potential of CZ-EO for developing new antimicrobial, antileishmanial, and antitrypanosomal drugs.https://www.mdpi.com/1424-8247/15/8/967Chrysopogon zizanioidesβ-eudesmolkhusimolLeishmania amazonensisperiodontopathogensTrypanosoma cruzi |
spellingShingle | Thaís A. S. Oliveira Tatiana M. Vieira Viviane R. Esperandim Carlos H. G. Martins Lizandra G. Magalhães Mayker L. D. Miranda Antônio E. M. Crotti Antibacterial, Antiparasitic, and Cytotoxic Activities of Chemical Characterized Essential Oil of <i>Chrysopogon zizanioides</i> Roots Pharmaceuticals Chrysopogon zizanioides β-eudesmol khusimol Leishmania amazonensis periodontopathogens Trypanosoma cruzi |
title | Antibacterial, Antiparasitic, and Cytotoxic Activities of Chemical Characterized Essential Oil of <i>Chrysopogon zizanioides</i> Roots |
title_full | Antibacterial, Antiparasitic, and Cytotoxic Activities of Chemical Characterized Essential Oil of <i>Chrysopogon zizanioides</i> Roots |
title_fullStr | Antibacterial, Antiparasitic, and Cytotoxic Activities of Chemical Characterized Essential Oil of <i>Chrysopogon zizanioides</i> Roots |
title_full_unstemmed | Antibacterial, Antiparasitic, and Cytotoxic Activities of Chemical Characterized Essential Oil of <i>Chrysopogon zizanioides</i> Roots |
title_short | Antibacterial, Antiparasitic, and Cytotoxic Activities of Chemical Characterized Essential Oil of <i>Chrysopogon zizanioides</i> Roots |
title_sort | antibacterial antiparasitic and cytotoxic activities of chemical characterized essential oil of i chrysopogon zizanioides i roots |
topic | Chrysopogon zizanioides β-eudesmol khusimol Leishmania amazonensis periodontopathogens Trypanosoma cruzi |
url | https://www.mdpi.com/1424-8247/15/8/967 |
work_keys_str_mv | AT thaisasoliveira antibacterialantiparasiticandcytotoxicactivitiesofchemicalcharacterizedessentialoilofichrysopogonzizanioidesiroots AT tatianamvieira antibacterialantiparasiticandcytotoxicactivitiesofchemicalcharacterizedessentialoilofichrysopogonzizanioidesiroots AT vivianeresperandim antibacterialantiparasiticandcytotoxicactivitiesofchemicalcharacterizedessentialoilofichrysopogonzizanioidesiroots AT carloshgmartins antibacterialantiparasiticandcytotoxicactivitiesofchemicalcharacterizedessentialoilofichrysopogonzizanioidesiroots AT lizandragmagalhaes antibacterialantiparasiticandcytotoxicactivitiesofchemicalcharacterizedessentialoilofichrysopogonzizanioidesiroots AT maykerldmiranda antibacterialantiparasiticandcytotoxicactivitiesofchemicalcharacterizedessentialoilofichrysopogonzizanioidesiroots AT antonioemcrotti antibacterialantiparasiticandcytotoxicactivitiesofchemicalcharacterizedessentialoilofichrysopogonzizanioidesiroots |