Multi-Omics Profiling of <i>Candida albicans</i> Grown on Solid Versus Liquid Media
<i>Candida albicans</i> is a common pathogenic fungus that presents a challenge to healthcare facilities. It can switch between a yeast cell form that diffuses through the bloodstream to colonize internal organs and a filamentous form that penetrates host mucosa. Understanding the pathog...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/11/12/2831 |
| _version_ | 1797379968774176768 |
|---|---|
| author | Rouba Abdulsalam Alhameed Mohammad H. Semreen Mohamad Hamad Alexander D. Giddey Ashna Sulaiman Mohammad T. Al Bataineh Hamza M. Al-Hroub Yasser Bustanji Karem H. Alzoubi Nelson C. Soares |
| author_facet | Rouba Abdulsalam Alhameed Mohammad H. Semreen Mohamad Hamad Alexander D. Giddey Ashna Sulaiman Mohammad T. Al Bataineh Hamza M. Al-Hroub Yasser Bustanji Karem H. Alzoubi Nelson C. Soares |
| author_sort | Rouba Abdulsalam Alhameed |
| collection | DOAJ |
| description | <i>Candida albicans</i> is a common pathogenic fungus that presents a challenge to healthcare facilities. It can switch between a yeast cell form that diffuses through the bloodstream to colonize internal organs and a filamentous form that penetrates host mucosa. Understanding the pathogen’s strategies for environmental adaptation and, ultimately, survival, is crucial. As a complementary study, herein, a multi-omics analysis was performed using high-resolution timsTOF MS to compare the proteomes and metabolomes of Wild Type (WT) <i>Candida albicans</i> (strain DK318) grown on agar plates versus liquid media. Proteomic analysis revealed a total of 1793 proteins and 15,013 peptides. Out of the 1403 identified proteins, 313 proteins were significantly differentially abundant with a <i>p</i>-value < 0.05. Of these, 156 and 157 proteins were significantly increased in liquid and solid media, respectively. Metabolomics analysis identified 192 metabolites in total. The majority (42/48) of the significantly altered metabolites (<i>p</i>-value 0.05 FDR, FC 1.5), mainly amino acids, were significantly higher in solid media, while only 2 metabolites were significantly higher in liquid media. The combined multi-omics analysis provides insight into adaptative morphological changes supporting <i>Candida albicans</i>’ life cycle and identifies crucial virulence factors during biofilm formation and bloodstream infection. |
| first_indexed | 2024-03-08T20:31:41Z |
| format | Article |
| id | doaj.art-27dc284127904f609ccaa289bf6bd0b2 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-08T20:31:41Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-27dc284127904f609ccaa289bf6bd0b22023-12-22T14:25:37ZengMDPI AGMicroorganisms2076-26072023-11-011112283110.3390/microorganisms11122831Multi-Omics Profiling of <i>Candida albicans</i> Grown on Solid Versus Liquid MediaRouba Abdulsalam Alhameed0Mohammad H. Semreen1Mohamad Hamad2Alexander D. Giddey3Ashna Sulaiman4Mohammad T. Al Bataineh5Hamza M. Al-Hroub6Yasser Bustanji7Karem H. Alzoubi8Nelson C. Soares9Research Institute of Medical and Health Sciences, University of Sharjah, Sharjah P.O. Box 27227, United Arab EmiratesResearch Institute of Medical and Health Sciences, University of Sharjah, Sharjah P.O. Box 27227, United Arab EmiratesResearch Institute of Medical and Health Sciences, University of Sharjah, Sharjah P.O. Box 27227, United Arab EmiratesCenter for Applied and Translational Genomics, Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai P.O. Box 505055, United Arab EmiratesResearch Institute of Medical and Health Sciences, University of Sharjah, Sharjah P.O. Box 27227, United Arab EmiratesCenter for Biotechnology, Department of Molecular Biology and Genetics, College of Medicine and Health Sciences, Khalifa University, Abu Dhabi P.O. Box 127788, United Arab EmiratesResearch Institute of Medical and Health Sciences, University of Sharjah, Sharjah P.O. Box 27227, United Arab EmiratesResearch Institute of Medical and Health Sciences, University of Sharjah, Sharjah P.O. Box 27227, United Arab EmiratesResearch Institute of Medical and Health Sciences, University of Sharjah, Sharjah P.O. Box 27227, United Arab EmiratesResearch Institute of Medical and Health Sciences, University of Sharjah, Sharjah P.O. Box 27227, United Arab Emirates<i>Candida albicans</i> is a common pathogenic fungus that presents a challenge to healthcare facilities. It can switch between a yeast cell form that diffuses through the bloodstream to colonize internal organs and a filamentous form that penetrates host mucosa. Understanding the pathogen’s strategies for environmental adaptation and, ultimately, survival, is crucial. As a complementary study, herein, a multi-omics analysis was performed using high-resolution timsTOF MS to compare the proteomes and metabolomes of Wild Type (WT) <i>Candida albicans</i> (strain DK318) grown on agar plates versus liquid media. Proteomic analysis revealed a total of 1793 proteins and 15,013 peptides. Out of the 1403 identified proteins, 313 proteins were significantly differentially abundant with a <i>p</i>-value < 0.05. Of these, 156 and 157 proteins were significantly increased in liquid and solid media, respectively. Metabolomics analysis identified 192 metabolites in total. The majority (42/48) of the significantly altered metabolites (<i>p</i>-value 0.05 FDR, FC 1.5), mainly amino acids, were significantly higher in solid media, while only 2 metabolites were significantly higher in liquid media. The combined multi-omics analysis provides insight into adaptative morphological changes supporting <i>Candida albicans</i>’ life cycle and identifies crucial virulence factors during biofilm formation and bloodstream infection.https://www.mdpi.com/2076-2607/11/12/2831<i>Candida albicans</i>proteomicsmetabolomicsLC-MS/MSenvironmental adaption |
| spellingShingle | Rouba Abdulsalam Alhameed Mohammad H. Semreen Mohamad Hamad Alexander D. Giddey Ashna Sulaiman Mohammad T. Al Bataineh Hamza M. Al-Hroub Yasser Bustanji Karem H. Alzoubi Nelson C. Soares Multi-Omics Profiling of <i>Candida albicans</i> Grown on Solid Versus Liquid Media Microorganisms <i>Candida albicans</i> proteomics metabolomics LC-MS/MS environmental adaption |
| title | Multi-Omics Profiling of <i>Candida albicans</i> Grown on Solid Versus Liquid Media |
| title_full | Multi-Omics Profiling of <i>Candida albicans</i> Grown on Solid Versus Liquid Media |
| title_fullStr | Multi-Omics Profiling of <i>Candida albicans</i> Grown on Solid Versus Liquid Media |
| title_full_unstemmed | Multi-Omics Profiling of <i>Candida albicans</i> Grown on Solid Versus Liquid Media |
| title_short | Multi-Omics Profiling of <i>Candida albicans</i> Grown on Solid Versus Liquid Media |
| title_sort | multi omics profiling of i candida albicans i grown on solid versus liquid media |
| topic | <i>Candida albicans</i> proteomics metabolomics LC-MS/MS environmental adaption |
| url | https://www.mdpi.com/2076-2607/11/12/2831 |
| work_keys_str_mv | AT roubaabdulsalamalhameed multiomicsprofilingoficandidaalbicansigrownonsolidversusliquidmedia AT mohammadhsemreen multiomicsprofilingoficandidaalbicansigrownonsolidversusliquidmedia AT mohamadhamad multiomicsprofilingoficandidaalbicansigrownonsolidversusliquidmedia AT alexanderdgiddey multiomicsprofilingoficandidaalbicansigrownonsolidversusliquidmedia AT ashnasulaiman multiomicsprofilingoficandidaalbicansigrownonsolidversusliquidmedia AT mohammadtalbataineh multiomicsprofilingoficandidaalbicansigrownonsolidversusliquidmedia AT hamzamalhroub multiomicsprofilingoficandidaalbicansigrownonsolidversusliquidmedia AT yasserbustanji multiomicsprofilingoficandidaalbicansigrownonsolidversusliquidmedia AT karemhalzoubi multiomicsprofilingoficandidaalbicansigrownonsolidversusliquidmedia AT nelsoncsoares multiomicsprofilingoficandidaalbicansigrownonsolidversusliquidmedia |