Molecular characterization and expression analysis of a novel C-type lectin (CTL) gene in yellow catfish Pelteobagrus fulvidraco
C-type lectin (CTL) proteins contain special carbohydrate-recognition domains (CRDs) and play vital roles in activating the innate immune system. In this study, a CTL gene from freshwater yellow catfish (Pelteobagrus fulvidraco) was identified and named as PfCTL. Analysis revealed that the complete...
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| Language: | English |
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Elsevier
2021-07-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513421000569 |
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| author | Xin-Hui Tang Xiao-Ting Ye Qi-Cheng Wu Ying-Yu Tang Dai-Zhen Zhang Qiu-Ning Liu Bo-Ping Tang Hao-Ru Zhu |
| author_facet | Xin-Hui Tang Xiao-Ting Ye Qi-Cheng Wu Ying-Yu Tang Dai-Zhen Zhang Qiu-Ning Liu Bo-Ping Tang Hao-Ru Zhu |
| author_sort | Xin-Hui Tang |
| collection | DOAJ |
| description | C-type lectin (CTL) proteins contain special carbohydrate-recognition domains (CRDs) and play vital roles in activating the innate immune system. In this study, a CTL gene from freshwater yellow catfish (Pelteobagrus fulvidraco) was identified and named as PfCTL. Analysis revealed that the complete PfCTL cDNA was 718 bp in size, including a 42-bp 5′-untranslated region (UTR), 61-bp 3′-UTR, and 615-bp open reading frame (ORF) encoding a 204 amino acid polypeptide, which contained a signal peptide and CRD. The PfCTL protein contained two casein kinase II phosphorylation sites, four protein kinase C phosphorylation sites, and two N-myristoylation sites. The deduced PfCTL protein sequence has 37–83 % in comparison to CTL proteins from other fish. Further phylogenetic analysis revealed that PfCTL was closely related to the CTL of Ictalurus punctatus. Based on quantitative real-time polymerase chain reaction, the PfCTL gene was expressed in all tested tissues at different levels, with highest distribution in the spleen, followed by the head kidney, and lowest distribution in blood. After challenge with lipopolysaccharide and polyriboinosinic polyribocytidylic acid, the expression of PfCTL was up-regulated in the liver, spleen, head kidney, and blood at different time points. These results imply that this CTL gene may be an important pathogen pattern recognition molecule in the innate immune system of yellow catfish under pathogen stimulation. |
| first_indexed | 2024-12-17T03:07:23Z |
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| id | doaj.art-27dd5a42c878416a817c705966499681 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-12-17T03:07:23Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-27dd5a42c878416a817c7059664996812022-12-21T22:05:55ZengElsevierAquaculture Reports2352-51342021-07-0120100640Molecular characterization and expression analysis of a novel C-type lectin (CTL) gene in yellow catfish Pelteobagrus fulvidracoXin-Hui Tang0Xiao-Ting Ye1Qi-Cheng Wu2Ying-Yu Tang3Dai-Zhen Zhang4Qiu-Ning Liu5Bo-Ping Tang6Hao-Ru Zhu7Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng, 224007, People's Republic of China; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, People's Republic of China; College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 210009, People's Republic of ChinaDepartment of Infectious Disease, Ruian People's Hospital, Wenzhou, 325200, People's Republic of ChinaJiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng, 224007, People's Republic of ChinaJiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng, 224007, People's Republic of China; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, People's Republic of China; College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 210009, People's Republic of ChinaJiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng, 224007, People's Republic of ChinaJiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng, 224007, People's Republic of China; Corresponding authors.Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng, 224007, People's Republic of China; Corresponding authors.School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, People's Republic of China; Corresponding authors.C-type lectin (CTL) proteins contain special carbohydrate-recognition domains (CRDs) and play vital roles in activating the innate immune system. In this study, a CTL gene from freshwater yellow catfish (Pelteobagrus fulvidraco) was identified and named as PfCTL. Analysis revealed that the complete PfCTL cDNA was 718 bp in size, including a 42-bp 5′-untranslated region (UTR), 61-bp 3′-UTR, and 615-bp open reading frame (ORF) encoding a 204 amino acid polypeptide, which contained a signal peptide and CRD. The PfCTL protein contained two casein kinase II phosphorylation sites, four protein kinase C phosphorylation sites, and two N-myristoylation sites. The deduced PfCTL protein sequence has 37–83 % in comparison to CTL proteins from other fish. Further phylogenetic analysis revealed that PfCTL was closely related to the CTL of Ictalurus punctatus. Based on quantitative real-time polymerase chain reaction, the PfCTL gene was expressed in all tested tissues at different levels, with highest distribution in the spleen, followed by the head kidney, and lowest distribution in blood. After challenge with lipopolysaccharide and polyriboinosinic polyribocytidylic acid, the expression of PfCTL was up-regulated in the liver, spleen, head kidney, and blood at different time points. These results imply that this CTL gene may be an important pathogen pattern recognition molecule in the innate immune system of yellow catfish under pathogen stimulation.http://www.sciencedirect.com/science/article/pii/S2352513421000569C-type lectinPelteobagrus fulvidracoExpression analysisImmune response |
| spellingShingle | Xin-Hui Tang Xiao-Ting Ye Qi-Cheng Wu Ying-Yu Tang Dai-Zhen Zhang Qiu-Ning Liu Bo-Ping Tang Hao-Ru Zhu Molecular characterization and expression analysis of a novel C-type lectin (CTL) gene in yellow catfish Pelteobagrus fulvidraco Aquaculture Reports C-type lectin Pelteobagrus fulvidraco Expression analysis Immune response |
| title | Molecular characterization and expression analysis of a novel C-type lectin (CTL) gene in yellow catfish Pelteobagrus fulvidraco |
| title_full | Molecular characterization and expression analysis of a novel C-type lectin (CTL) gene in yellow catfish Pelteobagrus fulvidraco |
| title_fullStr | Molecular characterization and expression analysis of a novel C-type lectin (CTL) gene in yellow catfish Pelteobagrus fulvidraco |
| title_full_unstemmed | Molecular characterization and expression analysis of a novel C-type lectin (CTL) gene in yellow catfish Pelteobagrus fulvidraco |
| title_short | Molecular characterization and expression analysis of a novel C-type lectin (CTL) gene in yellow catfish Pelteobagrus fulvidraco |
| title_sort | molecular characterization and expression analysis of a novel c type lectin ctl gene in yellow catfish pelteobagrus fulvidraco |
| topic | C-type lectin Pelteobagrus fulvidraco Expression analysis Immune response |
| url | http://www.sciencedirect.com/science/article/pii/S2352513421000569 |
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