Assessment of TCF7L2 expression after bariatric surgery.
<h4>Objective</h4>To assess the influence of bariatric surgery on transcription factor 7-like 2 (TCF7L2) expression and its association with body mass index (BMI) and Type 2 diabetes mellitus (T2DM).<h4>Methods</h4>Prospective study performed between 2016 and 2018, where 26 o...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0216627 |
| _version_ | 1818398697873997824 |
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| author | Carlos Eduardo S Macedo Guilherme da Conti Andriu S Catena Danyelly Bruneska Malu Rosa Clarissa G Noronha Fernando Santa Cruz Álvaro A B Ferraz |
| author_facet | Carlos Eduardo S Macedo Guilherme da Conti Andriu S Catena Danyelly Bruneska Malu Rosa Clarissa G Noronha Fernando Santa Cruz Álvaro A B Ferraz |
| author_sort | Carlos Eduardo S Macedo |
| collection | DOAJ |
| description | <h4>Objective</h4>To assess the influence of bariatric surgery on transcription factor 7-like 2 (TCF7L2) expression and its association with body mass index (BMI) and Type 2 diabetes mellitus (T2DM).<h4>Methods</h4>Prospective study performed between 2016 and 2018, where 26 obese patients undergoing bariatric surgery were divided into two subgroups: diabetics and non-diabetics. The RNAs were extracted from peripheral blood samples that were obtained from each patient in two different moments: before surgery and after 12 months of follow-up. The relative expression of TCF7L2 was determined according to the delta-Ct method.<h4>Results</h4>The linear regression model of BMI x delta-Ct showed a positive correlation (p = 0.037). In the subgroups, an inversely proportional relationship was found between delta-Ct and BMI in the diabetic group and a directly proportional relationship in the non-diabetic group (p>0.05 in both). In the postoperative period, the regression model was similar to the preoperative, except when analyzing the subgroups, where diabetic patients showed a directly proportional relationship (p>0.05). The relative expression of TCF7L2 showed an average of 1.16 ± 0.91, CI-95% 0.79-1.53. There was an increase in relative expression of 48% in the non-diabetic group (p = 0.021), and a decrease of 27% in the T2DM group (p>0.05) in the postoperative. There was a positive correlation between a greater decrease in BMI and increased relative expression (p = 0.027).<h4>Conclusion</h4>Our results showed that generally, the TCF7L2 expression increase with a decrease in BMI, however, for patients with T2DM, it exhibits an inverse pattern, which is normalized one year after bariatric surgery. |
| first_indexed | 2024-12-14T07:08:55Z |
| format | Article |
| id | doaj.art-27dfee22e47d42c29cd29668fd37d103 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T07:08:55Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-27dfee22e47d42c29cd29668fd37d1032022-12-21T23:12:02ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01145e021662710.1371/journal.pone.0216627Assessment of TCF7L2 expression after bariatric surgery.Carlos Eduardo S MacedoGuilherme da ContiAndriu S CatenaDanyelly BruneskaMalu RosaClarissa G NoronhaFernando Santa CruzÁlvaro A B Ferraz<h4>Objective</h4>To assess the influence of bariatric surgery on transcription factor 7-like 2 (TCF7L2) expression and its association with body mass index (BMI) and Type 2 diabetes mellitus (T2DM).<h4>Methods</h4>Prospective study performed between 2016 and 2018, where 26 obese patients undergoing bariatric surgery were divided into two subgroups: diabetics and non-diabetics. The RNAs were extracted from peripheral blood samples that were obtained from each patient in two different moments: before surgery and after 12 months of follow-up. The relative expression of TCF7L2 was determined according to the delta-Ct method.<h4>Results</h4>The linear regression model of BMI x delta-Ct showed a positive correlation (p = 0.037). In the subgroups, an inversely proportional relationship was found between delta-Ct and BMI in the diabetic group and a directly proportional relationship in the non-diabetic group (p>0.05 in both). In the postoperative period, the regression model was similar to the preoperative, except when analyzing the subgroups, where diabetic patients showed a directly proportional relationship (p>0.05). The relative expression of TCF7L2 showed an average of 1.16 ± 0.91, CI-95% 0.79-1.53. There was an increase in relative expression of 48% in the non-diabetic group (p = 0.021), and a decrease of 27% in the T2DM group (p>0.05) in the postoperative. There was a positive correlation between a greater decrease in BMI and increased relative expression (p = 0.027).<h4>Conclusion</h4>Our results showed that generally, the TCF7L2 expression increase with a decrease in BMI, however, for patients with T2DM, it exhibits an inverse pattern, which is normalized one year after bariatric surgery.https://doi.org/10.1371/journal.pone.0216627 |
| spellingShingle | Carlos Eduardo S Macedo Guilherme da Conti Andriu S Catena Danyelly Bruneska Malu Rosa Clarissa G Noronha Fernando Santa Cruz Álvaro A B Ferraz Assessment of TCF7L2 expression after bariatric surgery. PLoS ONE |
| title | Assessment of TCF7L2 expression after bariatric surgery. |
| title_full | Assessment of TCF7L2 expression after bariatric surgery. |
| title_fullStr | Assessment of TCF7L2 expression after bariatric surgery. |
| title_full_unstemmed | Assessment of TCF7L2 expression after bariatric surgery. |
| title_short | Assessment of TCF7L2 expression after bariatric surgery. |
| title_sort | assessment of tcf7l2 expression after bariatric surgery |
| url | https://doi.org/10.1371/journal.pone.0216627 |
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