Non-invasive genetic sampling for molecular sexing and microsatellite genotyping of hyacinth macaw (Anodorhynchus hyacinthinus)
Molted feather sampling is a useful tool for genetic analyses of endangered species, but it is often very laborious due to the low quality and quantity of the DNA obtained. In the present study we show the parts of feathers that resulted in better yield of DNA. In descending order these were: blood...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2013-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100019 |
| _version_ | 1819267100224520192 |
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| author | Flavia T. Presti Janaína Meyer Paulo T.Z. Antas Neiva M.R. Guedes Cristina Y. Miyaki |
| author_facet | Flavia T. Presti Janaína Meyer Paulo T.Z. Antas Neiva M.R. Guedes Cristina Y. Miyaki |
| author_sort | Flavia T. Presti |
| collection | DOAJ |
| description | Molted feather sampling is a useful tool for genetic analyses of endangered species, but it is often very laborious due to the low quality and quantity of the DNA obtained. In the present study we show the parts of feathers that resulted in better yield of DNA. In descending order these were: blood clot outside the umbilicus, umbilicus (without blood clot), tip, inner membrane, and small calamus. Compared to DNA extracted from blood samples, DNA extracted from feathers produced microsatellite alleles of poorer quality and had to be processed immediately after extraction. As expected due to the level of DNA degradation, molecular sexing protocols that result in shorter PCR products were more efficient. |
| first_indexed | 2024-12-23T21:11:48Z |
| format | Article |
| id | doaj.art-27dffedef03b444582a3eb9c1b399d6f |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-23T21:11:48Z |
| publishDate | 2013-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-27dffedef03b444582a3eb9c1b399d6f2022-12-21T17:31:03ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852013-01-01361129133Non-invasive genetic sampling for molecular sexing and microsatellite genotyping of hyacinth macaw (Anodorhynchus hyacinthinus)Flavia T. PrestiJanaína MeyerPaulo T.Z. AntasNeiva M.R. GuedesCristina Y. MiyakiMolted feather sampling is a useful tool for genetic analyses of endangered species, but it is often very laborious due to the low quality and quantity of the DNA obtained. In the present study we show the parts of feathers that resulted in better yield of DNA. In descending order these were: blood clot outside the umbilicus, umbilicus (without blood clot), tip, inner membrane, and small calamus. Compared to DNA extracted from blood samples, DNA extracted from feathers produced microsatellite alleles of poorer quality and had to be processed immediately after extraction. As expected due to the level of DNA degradation, molecular sexing protocols that result in shorter PCR products were more efficient.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100019molted feathersblood sampleDNAPCR productparrot |
| spellingShingle | Flavia T. Presti Janaína Meyer Paulo T.Z. Antas Neiva M.R. Guedes Cristina Y. Miyaki Non-invasive genetic sampling for molecular sexing and microsatellite genotyping of hyacinth macaw (Anodorhynchus hyacinthinus) Genetics and Molecular Biology molted feathers blood sample DNA PCR product parrot |
| title | Non-invasive genetic sampling for molecular sexing and microsatellite genotyping of hyacinth macaw (Anodorhynchus hyacinthinus) |
| title_full | Non-invasive genetic sampling for molecular sexing and microsatellite genotyping of hyacinth macaw (Anodorhynchus hyacinthinus) |
| title_fullStr | Non-invasive genetic sampling for molecular sexing and microsatellite genotyping of hyacinth macaw (Anodorhynchus hyacinthinus) |
| title_full_unstemmed | Non-invasive genetic sampling for molecular sexing and microsatellite genotyping of hyacinth macaw (Anodorhynchus hyacinthinus) |
| title_short | Non-invasive genetic sampling for molecular sexing and microsatellite genotyping of hyacinth macaw (Anodorhynchus hyacinthinus) |
| title_sort | non invasive genetic sampling for molecular sexing and microsatellite genotyping of hyacinth macaw anodorhynchus hyacinthinus |
| topic | molted feathers blood sample DNA PCR product parrot |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100019 |
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