Evaluation and Screening of Hypoglycemic Activity of Total Ginsenosides GBE-5 Fraction From Panax Ginseng Berry Based on UHPLC–MS Metabolomics
ObjectiveGinseng berry (GB) was the mature fruit of medicinal and edible herb, Panax ginseng C.A. Meyer, with significant hypoglycemic effect. Ginsenoside was the main hypoglycemic active component of GB. Evaluating and screening the effective components of GB was of great significance to further de...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.865077/full |
| _version_ | 1818141476377329664 |
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| author | Heyu Wang Heyu Wang Yu Tong Anqi Wang Ying Li Bofan Lu Hui Li Lili Jiao Wei Wu |
| author_facet | Heyu Wang Heyu Wang Yu Tong Anqi Wang Ying Li Bofan Lu Hui Li Lili Jiao Wei Wu |
| author_sort | Heyu Wang |
| collection | DOAJ |
| description | ObjectiveGinseng berry (GB) was the mature fruit of medicinal and edible herb, Panax ginseng C.A. Meyer, with significant hypoglycemic effect. Ginsenoside was the main hypoglycemic active component of GB. Evaluating and screening the effective components of GB was of great significance to further develop its hypoglycemic effect.MethodsThe polar fractions of ginseng berry extract (GBE) were separated by a solvent extraction, and identified by ultra-high performance liquid chromatography–high-resolution mass spectrometry (UHPLC–MS). The insulin resistance model of HepG2 cells was established, and the hypoglycemic active fraction in GBE polar fractions were screened in vitro. Rat model of type 2 diabetes mellitus (T2DM) was established to verify the hypoglycemic effect of the GBE active fraction. The metabolomic study based on UHPLC–MS was used to analyze the differential metabolites in the serum of T2DM rats after 30 days of intervention with hypoglycemic active GBE fraction. The kyoto encyclopedia of genes and genomes (KEGG) metabolic pathway enrichment analysis was used to study the main metabolic pathways involved in the regulation of hypoglycemic active parts of GBE.ResultsIt was found that GBE-5 fraction had better hypoglycemic activity than other GBE polar fractions in vitro cell hypoglycemic activity screening experiment. After 30 days of treatment, the fasting blood glucose value of T2DM rats decreased significantly by 34.75%, indicating that it had significant hypoglycemic effect. Eighteen differential metabolites enriched in KEGG metabolic pathway were screened and identified in the rat serum from T2DM vs. GBE-5 group, and the metabolic pathways mainly involved in regulation include arachidonic acid (AA) metabolism, linoleic acid (LA) metabolism, unsaturated fatty acid biosynthesis, and ferroptosis.ConclusionsThe hypoglycemic effect of GBE-5 fraction was better than that of total ginsenoside of GB. The AA metabolism, LA metabolism, unsaturated fatty acid biosynthesis, and ferroptosis were the potential metabolic pathways for GBE-5 fraction to exert hypoglycemic regulation. |
| first_indexed | 2024-12-11T11:00:29Z |
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| id | doaj.art-27e39bf0062747f8bb2a3c5fcd1d288d |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-11T11:00:29Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-27e39bf0062747f8bb2a3c5fcd1d288d2022-12-22T01:09:53ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-04-01910.3389/fnut.2022.865077865077Evaluation and Screening of Hypoglycemic Activity of Total Ginsenosides GBE-5 Fraction From Panax Ginseng Berry Based on UHPLC–MS MetabolomicsHeyu Wang0Heyu Wang1Yu Tong2Anqi Wang3Ying Li4Bofan Lu5Hui Li6Lili Jiao7Wei Wu8Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, ChinaSchool of Pharmacy, Jilin Medical University, Jilin, ChinaJilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, ChinaJilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, ChinaJilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, ChinaJilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, ChinaJilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, ChinaJilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, ChinaJilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, ChinaObjectiveGinseng berry (GB) was the mature fruit of medicinal and edible herb, Panax ginseng C.A. Meyer, with significant hypoglycemic effect. Ginsenoside was the main hypoglycemic active component of GB. Evaluating and screening the effective components of GB was of great significance to further develop its hypoglycemic effect.MethodsThe polar fractions of ginseng berry extract (GBE) were separated by a solvent extraction, and identified by ultra-high performance liquid chromatography–high-resolution mass spectrometry (UHPLC–MS). The insulin resistance model of HepG2 cells was established, and the hypoglycemic active fraction in GBE polar fractions were screened in vitro. Rat model of type 2 diabetes mellitus (T2DM) was established to verify the hypoglycemic effect of the GBE active fraction. The metabolomic study based on UHPLC–MS was used to analyze the differential metabolites in the serum of T2DM rats after 30 days of intervention with hypoglycemic active GBE fraction. The kyoto encyclopedia of genes and genomes (KEGG) metabolic pathway enrichment analysis was used to study the main metabolic pathways involved in the regulation of hypoglycemic active parts of GBE.ResultsIt was found that GBE-5 fraction had better hypoglycemic activity than other GBE polar fractions in vitro cell hypoglycemic activity screening experiment. After 30 days of treatment, the fasting blood glucose value of T2DM rats decreased significantly by 34.75%, indicating that it had significant hypoglycemic effect. Eighteen differential metabolites enriched in KEGG metabolic pathway were screened and identified in the rat serum from T2DM vs. GBE-5 group, and the metabolic pathways mainly involved in regulation include arachidonic acid (AA) metabolism, linoleic acid (LA) metabolism, unsaturated fatty acid biosynthesis, and ferroptosis.ConclusionsThe hypoglycemic effect of GBE-5 fraction was better than that of total ginsenoside of GB. The AA metabolism, LA metabolism, unsaturated fatty acid biosynthesis, and ferroptosis were the potential metabolic pathways for GBE-5 fraction to exert hypoglycemic regulation.https://www.frontiersin.org/articles/10.3389/fnut.2022.865077/fullginseng berryginsenosideUHPLC-MSmetabolomicstype 2 diabetes mellitus |
| spellingShingle | Heyu Wang Heyu Wang Yu Tong Anqi Wang Ying Li Bofan Lu Hui Li Lili Jiao Wei Wu Evaluation and Screening of Hypoglycemic Activity of Total Ginsenosides GBE-5 Fraction From Panax Ginseng Berry Based on UHPLC–MS Metabolomics Frontiers in Nutrition ginseng berry ginsenoside UHPLC-MS metabolomics type 2 diabetes mellitus |
| title | Evaluation and Screening of Hypoglycemic Activity of Total Ginsenosides GBE-5 Fraction From Panax Ginseng Berry Based on UHPLC–MS Metabolomics |
| title_full | Evaluation and Screening of Hypoglycemic Activity of Total Ginsenosides GBE-5 Fraction From Panax Ginseng Berry Based on UHPLC–MS Metabolomics |
| title_fullStr | Evaluation and Screening of Hypoglycemic Activity of Total Ginsenosides GBE-5 Fraction From Panax Ginseng Berry Based on UHPLC–MS Metabolomics |
| title_full_unstemmed | Evaluation and Screening of Hypoglycemic Activity of Total Ginsenosides GBE-5 Fraction From Panax Ginseng Berry Based on UHPLC–MS Metabolomics |
| title_short | Evaluation and Screening of Hypoglycemic Activity of Total Ginsenosides GBE-5 Fraction From Panax Ginseng Berry Based on UHPLC–MS Metabolomics |
| title_sort | evaluation and screening of hypoglycemic activity of total ginsenosides gbe 5 fraction from panax ginseng berry based on uhplc ms metabolomics |
| topic | ginseng berry ginsenoside UHPLC-MS metabolomics type 2 diabetes mellitus |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.865077/full |
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